Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression
Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n10_p_Gonzalez http://hdl.handle.net/20.500.12110/paper_19326203_v7_n10_p_Gonzalez |
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paper:paper_19326203_v7_n10_p_Gonzalez2023-06-08T16:30:59Z Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. © 2012 González et al. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n10_p_Gonzalez http://hdl.handle.net/20.500.12110/paper_19326203_v7_n10_p_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus |
| spellingShingle |
angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression |
| topic_facet |
angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus |
| description |
Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. © 2012 González et al. |
| title |
Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression |
| title_short |
Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression |
| title_full |
Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression |
| title_fullStr |
Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression |
| title_full_unstemmed |
Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression |
| title_sort |
uterine nk cells are critical in shaping dc immunogenic functions compatible with pregnancy progression |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n10_p_Gonzalez http://hdl.handle.net/20.500.12110/paper_19326203_v7_n10_p_Gonzalez |
| _version_ |
1768546089202352128 |