The role of pH fronts in reversible electroporation

We present experimental measurements and theoretical predictions of ion transport in agar gels during reversible electroporation (ECT) for conditions typical to many clinical studies found in the literature, revealing the presence of pH fronts emerging from both electrodes. These results suggest tha...

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Autores principales:	Turjanski, Pablo Guillermo, Suárez, Cecilia Ana, Molina, Fernando Victor
Publicado:	2011
Materias:	acidification article comparative study controlled study electrode electrogenetherapy electroporation electrostimulation therapy gene therapy ion transport pH reversible electroporation tissue necrosis Animals Electrochemistry Electrodes Electroporation Genetic Techniques Genetic Therapy Humans Hydrogen-Ion Concentration Models, Theoretical Necrosis Plasmids Time Factors
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n4_p_Turjanski http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Turjanski
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_19326203_v6_n4_p_Turjanski
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spelling	paper:paper_19326203_v6_n4_p_Turjanski2023-06-08T16:30:56Z The role of pH fronts in reversible electroporation Turjanski, Pablo Guillermo Suárez, Cecilia Ana Molina, Fernando Victor acidification article comparative study controlled study electrode electrogenetherapy electroporation electrostimulation therapy gene therapy ion transport pH reversible electroporation tissue necrosis Animals Electrochemistry Electrodes Electroporation Genetic Techniques Genetic Therapy Humans Hydrogen-Ion Concentration Models, Theoretical Necrosis Plasmids Time Factors We present experimental measurements and theoretical predictions of ion transport in agar gels during reversible electroporation (ECT) for conditions typical to many clinical studies found in the literature, revealing the presence of pH fronts emerging from both electrodes. These results suggest that pH fronts are immediate and substantial. Since they might give rise to tissue necrosis, an unwanted condition in clinical applications of ECT as well as in irreversible electroporation (IRE) and in electrogenetherapy (EGT), it is important to quantify their extent and evolution. Here, a tracking technique is used to follow the space-time evolution of these pH fronts. It is found that they scale in time as t1/2 characteristic of a predominantly diffusive process. Comparing ECT pH fronts with those arising in electrotherapy (EChT), another treatment applying constant electric fields whose main goal is tissue necrosis, a striking result is observed: anodic acidification is larger in ECT than in EChT, suggesting that tissue necrosis could also be greater. Ways to minimize these adverse effects in ECT are suggested. © 2011 Turjanski et al. Fil:Turjanski, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Suárez, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molina, F.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n4_p_Turjanski http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Turjanski
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acidification article comparative study controlled study electrode electrogenetherapy electroporation electrostimulation therapy gene therapy ion transport pH reversible electroporation tissue necrosis Animals Electrochemistry Electrodes Electroporation Genetic Techniques Genetic Therapy Humans Hydrogen-Ion Concentration Models, Theoretical Necrosis Plasmids Time Factors
spellingShingle	acidification article comparative study controlled study electrode electrogenetherapy electroporation electrostimulation therapy gene therapy ion transport pH reversible electroporation tissue necrosis Animals Electrochemistry Electrodes Electroporation Genetic Techniques Genetic Therapy Humans Hydrogen-Ion Concentration Models, Theoretical Necrosis Plasmids Time Factors Turjanski, Pablo Guillermo Suárez, Cecilia Ana Molina, Fernando Victor The role of pH fronts in reversible electroporation
topic_facet	acidification article comparative study controlled study electrode electrogenetherapy electroporation electrostimulation therapy gene therapy ion transport pH reversible electroporation tissue necrosis Animals Electrochemistry Electrodes Electroporation Genetic Techniques Genetic Therapy Humans Hydrogen-Ion Concentration Models, Theoretical Necrosis Plasmids Time Factors
description	We present experimental measurements and theoretical predictions of ion transport in agar gels during reversible electroporation (ECT) for conditions typical to many clinical studies found in the literature, revealing the presence of pH fronts emerging from both electrodes. These results suggest that pH fronts are immediate and substantial. Since they might give rise to tissue necrosis, an unwanted condition in clinical applications of ECT as well as in irreversible electroporation (IRE) and in electrogenetherapy (EGT), it is important to quantify their extent and evolution. Here, a tracking technique is used to follow the space-time evolution of these pH fronts. It is found that they scale in time as t1/2 characteristic of a predominantly diffusive process. Comparing ECT pH fronts with those arising in electrotherapy (EChT), another treatment applying constant electric fields whose main goal is tissue necrosis, a striking result is observed: anodic acidification is larger in ECT than in EChT, suggesting that tissue necrosis could also be greater. Ways to minimize these adverse effects in ECT are suggested. © 2011 Turjanski et al.
author	Turjanski, Pablo Guillermo Suárez, Cecilia Ana Molina, Fernando Victor
author_facet	Turjanski, Pablo Guillermo Suárez, Cecilia Ana Molina, Fernando Victor
author_sort	Turjanski, Pablo Guillermo
title	The role of pH fronts in reversible electroporation
title_short	The role of pH fronts in reversible electroporation
title_full	The role of pH fronts in reversible electroporation
title_fullStr	The role of pH fronts in reversible electroporation
title_full_unstemmed	The role of pH fronts in reversible electroporation
title_sort	role of ph fronts in reversible electroporation
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n4_p_Turjanski http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Turjanski
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The role of pH fronts in reversible electroporation

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