Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells
The organization of the cytoplasm is regulated by molecular motors which transport organelles and other cargoes along cytoskeleton tracks. Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n4_p_Bruno http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Bruno |
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paper:paper_19326203_v6_n4_p_Bruno2023-06-08T16:30:56Z Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells Bruno, Luciana Salierno, Marcelo Javier Wetzler, Diana E. Despósito, Marcelo Arnaldo Levi, Valeria dynactin dynein adenosine triphosphatase kinesin molecular motor dynactin microtubule associated protein molecular motor animal cell article cell organelle cell tracking controlled study gene dosage in vitro study melanosome microtubule molecular dynamics nonhuman protein function protein interaction protein stiffness protein transport quantitative analysis viscoelasticity Xenopus laevis animal biological model biomechanics cell survival elasticity mechanics melanosome metabolism microtubule viscosity Animals Biomechanics Cell Survival Elasticity Mechanical Processes Melanosomes Microtubule-Associated Proteins Microtubules Models, Biological Molecular Motor Proteins Protein Transport Viscosity Xenopus laevis The organization of the cytoplasm is regulated by molecular motors which transport organelles and other cargoes along cytoskeleton tracks. Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-2, respectively. In this work, we used single particle tracking to characterize the mechanical properties of motor-driven organelles during transport along microtubules. We tracked organelles with high temporal and spatial resolutions and characterized their dynamics perpendicular to the cytoskeleton track. The quantitative analysis of these data showed that the dynamics is due to a spring-like interaction between melanosomes and microtubules in a viscoelastic microenvironment. A model based on a generalized Langevin equation explained these observations and predicted that the stiffness measured for the motor complex acting as a linker between organelles and microtubules is ~ one order smaller than that determined for motor proteins in vitro. This result suggests that other biomolecules involved in the interaction between motors and organelles contribute to the mechanical properties of the motor complex. We hypothesise that the high flexibility observed for the motor linker may be required to improve the efficiency of the transport driven by multiple copies of motor molecules. © 2011 Bruno et al. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salierno, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wetzler, D.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Despósito, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n4_p_Bruno http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Bruno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
dynactin dynein adenosine triphosphatase kinesin molecular motor dynactin microtubule associated protein molecular motor animal cell article cell organelle cell tracking controlled study gene dosage in vitro study melanosome microtubule molecular dynamics nonhuman protein function protein interaction protein stiffness protein transport quantitative analysis viscoelasticity Xenopus laevis animal biological model biomechanics cell survival elasticity mechanics melanosome metabolism microtubule viscosity Animals Biomechanics Cell Survival Elasticity Mechanical Processes Melanosomes Microtubule-Associated Proteins Microtubules Models, Biological Molecular Motor Proteins Protein Transport Viscosity Xenopus laevis |
| spellingShingle |
dynactin dynein adenosine triphosphatase kinesin molecular motor dynactin microtubule associated protein molecular motor animal cell article cell organelle cell tracking controlled study gene dosage in vitro study melanosome microtubule molecular dynamics nonhuman protein function protein interaction protein stiffness protein transport quantitative analysis viscoelasticity Xenopus laevis animal biological model biomechanics cell survival elasticity mechanics melanosome metabolism microtubule viscosity Animals Biomechanics Cell Survival Elasticity Mechanical Processes Melanosomes Microtubule-Associated Proteins Microtubules Models, Biological Molecular Motor Proteins Protein Transport Viscosity Xenopus laevis Bruno, Luciana Salierno, Marcelo Javier Wetzler, Diana E. Despósito, Marcelo Arnaldo Levi, Valeria Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells |
| topic_facet |
dynactin dynein adenosine triphosphatase kinesin molecular motor dynactin microtubule associated protein molecular motor animal cell article cell organelle cell tracking controlled study gene dosage in vitro study melanosome microtubule molecular dynamics nonhuman protein function protein interaction protein stiffness protein transport quantitative analysis viscoelasticity Xenopus laevis animal biological model biomechanics cell survival elasticity mechanics melanosome metabolism microtubule viscosity Animals Biomechanics Cell Survival Elasticity Mechanical Processes Melanosomes Microtubule-Associated Proteins Microtubules Models, Biological Molecular Motor Proteins Protein Transport Viscosity Xenopus laevis |
| description |
The organization of the cytoplasm is regulated by molecular motors which transport organelles and other cargoes along cytoskeleton tracks. Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-2, respectively. In this work, we used single particle tracking to characterize the mechanical properties of motor-driven organelles during transport along microtubules. We tracked organelles with high temporal and spatial resolutions and characterized their dynamics perpendicular to the cytoskeleton track. The quantitative analysis of these data showed that the dynamics is due to a spring-like interaction between melanosomes and microtubules in a viscoelastic microenvironment. A model based on a generalized Langevin equation explained these observations and predicted that the stiffness measured for the motor complex acting as a linker between organelles and microtubules is ~ one order smaller than that determined for motor proteins in vitro. This result suggests that other biomolecules involved in the interaction between motors and organelles contribute to the mechanical properties of the motor complex. We hypothesise that the high flexibility observed for the motor linker may be required to improve the efficiency of the transport driven by multiple copies of motor molecules. © 2011 Bruno et al. |
| author |
Bruno, Luciana Salierno, Marcelo Javier Wetzler, Diana E. Despósito, Marcelo Arnaldo Levi, Valeria |
| author_facet |
Bruno, Luciana Salierno, Marcelo Javier Wetzler, Diana E. Despósito, Marcelo Arnaldo Levi, Valeria |
| author_sort |
Bruno, Luciana |
| title |
Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells |
| title_short |
Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells |
| title_full |
Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells |
| title_fullStr |
Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells |
| title_full_unstemmed |
Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells |
| title_sort |
mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n4_p_Bruno http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Bruno |
| work_keys_str_mv |
AT brunoluciana mechanicalpropertiesoforganellesdrivenbymicrotubuledependentmolecularmotorsinlivingcells AT saliernomarcelojavier mechanicalpropertiesoforganellesdrivenbymicrotubuledependentmolecularmotorsinlivingcells AT wetzlerdianae mechanicalpropertiesoforganellesdrivenbymicrotubuledependentmolecularmotorsinlivingcells AT despositomarceloarnaldo mechanicalpropertiesoforganellesdrivenbymicrotubuledependentmolecularmotorsinlivingcells AT levivaleria mechanicalpropertiesoforganellesdrivenbymicrotubuledependentmolecularmotorsinlivingcells |
| _version_ |
1768543821125124096 |