A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells
Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct p...
Guardado en:
Autores principales: | , |
---|---|
Publicado: |
2009
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v4_n10_p_Galli http://hdl.handle.net/20.500.12110/paper_19326203_v4_n10_p_Galli |
Aporte de: |
id |
paper:paper_19326203_v4_n10_p_Galli |
---|---|
record_format |
dspace |
spelling |
paper:paper_19326203_v4_n10_p_Galli2023-06-08T16:30:51Z A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells Galli, Soledad Jares, Elizabeth Andrea histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct phosphorylation of downstream effectors located throughout the cell. In this study we determined that hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus. In the mitochondrial environment, hERK1 physically associates with (i) at least 5 mitochondrial proteins with functions related to transport (i.e. VDAC1), signalling, and metabolism; (ii) histones H2A and H4; and (iii) other cytosolic proteins. This work indicates for the first time the presence of diverse ERK-complexes in mitochondria and thus provides a new perspective for assessing the functions of ERK1 in the regulation of cellular signalling and trafficking in HeLa cells. © 2009 Galli et al. Fil:Galli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v4_n10_p_Galli http://hdl.handle.net/20.500.12110/paper_19326203_v4_n10_p_Galli |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
spellingShingle |
histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Galli, Soledad Jares, Elizabeth Andrea A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells |
topic_facet |
histone H2A histone H4 mitogen activated protein kinase 1 mitogen activated protein kinase 3 voltage dependent anion channel glutathione transferase mitogen activated protein kinase 3 article complex formation controlled study enzyme activity gene control gene expression gene location HeLa cell human human cell mitochondrion nucleotide sequence protein protein interaction protein transport signal transduction amino acid sequence cell proliferation gene expression profiling gene expression regulation mass spectrometry metabolism methodology molecular genetics proteomics sequence homology Amino Acid Sequence Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Glutathione Transferase Hela Cells Humans MAP Kinase Signaling System Mitochondria Mitogen-Activated Protein Kinase 3 Molecular Sequence Data Proteomics Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
description |
Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct phosphorylation of downstream effectors located throughout the cell. In this study we determined that hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus. In the mitochondrial environment, hERK1 physically associates with (i) at least 5 mitochondrial proteins with functions related to transport (i.e. VDAC1), signalling, and metabolism; (ii) histones H2A and H4; and (iii) other cytosolic proteins. This work indicates for the first time the presence of diverse ERK-complexes in mitochondria and thus provides a new perspective for assessing the functions of ERK1 in the regulation of cellular signalling and trafficking in HeLa cells. © 2009 Galli et al. |
author |
Galli, Soledad Jares, Elizabeth Andrea |
author_facet |
Galli, Soledad Jares, Elizabeth Andrea |
author_sort |
Galli, Soledad |
title |
A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells |
title_short |
A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells |
title_full |
A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells |
title_fullStr |
A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells |
title_full_unstemmed |
A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells |
title_sort |
new paradigm for mapk: structural interactions of herk1 with mitochondria in hela cells |
publishDate |
2009 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v4_n10_p_Galli http://hdl.handle.net/20.500.12110/paper_19326203_v4_n10_p_Galli |
work_keys_str_mv |
AT gallisoledad anewparadigmformapkstructuralinteractionsofherk1withmitochondriainhelacells AT jareselizabethandrea anewparadigmformapkstructuralinteractionsofherk1withmitochondriainhelacells AT gallisoledad newparadigmformapkstructuralinteractionsofherk1withmitochondriainhelacells AT jareselizabethandrea newparadigmformapkstructuralinteractionsofherk1withmitochondriainhelacells |
_version_ |
1768542760104624128 |