Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis
Psychrotroph microorganisms have developed cellular mechanisms to cope with cold stress. Cell envelopes are key components for bacterial survival. Outer membrane is a constituent of Gram negative bacterial envelopes, consisting of several components, such as lipopolysaccharides (LPS). In this work w...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v13_n2_p_Benforte http://hdl.handle.net/20.500.12110/paper_19326203_v13_n2_p_Benforte |
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paper:paper_19326203_v13_n2_p_Benforte2023-06-08T16:30:46Z Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis glycosyltransferase lipopolysaccharide glycosyltransferase lipopolysaccharide acclimatization Article bacterial growth bacterial strain cell flexibility cell function cell interaction cell membrane permeability cold acclimatization cold shock response controlled study limit of quantitation low temperature nonhuman phenotype Pseudomonas Pseudomonas extremaustralis turgor pressure adaptation Antarctica bacterial gene cold enzymology genetics metabolism physiology Pseudomonas real time polymerase chain reaction Adaptation, Physiological Antarctic Regions Cold Temperature Genes, Bacterial Glycosyltransferases Lipopolysaccharides Pseudomonas Real-Time Polymerase Chain Reaction Psychrotroph microorganisms have developed cellular mechanisms to cope with cold stress. Cell envelopes are key components for bacterial survival. Outer membrane is a constituent of Gram negative bacterial envelopes, consisting of several components, such as lipopolysaccharides (LPS). In this work we investigated the relevance of envelope characteristics for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis by analyzing a mini Tn5 wapH mutant strain, encoding a core LPS glycosyltransferase. Our results showed that wapH strain is impaired to grow under low temperature but not for cold survival. The mutation in wapH, provoked a strong aggregative phenotype and modifications of envelope nanomechanical properties such as lower flexibility and higher turgor pressure, cell permeability and surface area to volume ratio (S/V). Changes in these characteristics were also observed in the wild type strain grown at different temperatures, showing higher cell flexibility but lower turgor pressure under cold conditions. Cold shock experiments indicated that an acclimation period in the wild type is necessary for cell flexibility and S/V ratio adjustments. Alteration in cell-cell interaction capabilities was observed in wapH strain. Mixed cells of wild type and wapH strains, as well as those of the wild type strain grown at different temperatures, showed a mosaic pattern of aggregation. These results indicate that wapH mutation provoked marked envelope alterations showing that LPS core conservation appears as a novel essential feature for active growth under cold conditions. © 2018 Benforte et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v13_n2_p_Benforte http://hdl.handle.net/20.500.12110/paper_19326203_v13_n2_p_Benforte |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
glycosyltransferase lipopolysaccharide glycosyltransferase lipopolysaccharide acclimatization Article bacterial growth bacterial strain cell flexibility cell function cell interaction cell membrane permeability cold acclimatization cold shock response controlled study limit of quantitation low temperature nonhuman phenotype Pseudomonas Pseudomonas extremaustralis turgor pressure adaptation Antarctica bacterial gene cold enzymology genetics metabolism physiology Pseudomonas real time polymerase chain reaction Adaptation, Physiological Antarctic Regions Cold Temperature Genes, Bacterial Glycosyltransferases Lipopolysaccharides Pseudomonas Real-Time Polymerase Chain Reaction |
| spellingShingle |
glycosyltransferase lipopolysaccharide glycosyltransferase lipopolysaccharide acclimatization Article bacterial growth bacterial strain cell flexibility cell function cell interaction cell membrane permeability cold acclimatization cold shock response controlled study limit of quantitation low temperature nonhuman phenotype Pseudomonas Pseudomonas extremaustralis turgor pressure adaptation Antarctica bacterial gene cold enzymology genetics metabolism physiology Pseudomonas real time polymerase chain reaction Adaptation, Physiological Antarctic Regions Cold Temperature Genes, Bacterial Glycosyltransferases Lipopolysaccharides Pseudomonas Real-Time Polymerase Chain Reaction Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis |
| topic_facet |
glycosyltransferase lipopolysaccharide glycosyltransferase lipopolysaccharide acclimatization Article bacterial growth bacterial strain cell flexibility cell function cell interaction cell membrane permeability cold acclimatization cold shock response controlled study limit of quantitation low temperature nonhuman phenotype Pseudomonas Pseudomonas extremaustralis turgor pressure adaptation Antarctica bacterial gene cold enzymology genetics metabolism physiology Pseudomonas real time polymerase chain reaction Adaptation, Physiological Antarctic Regions Cold Temperature Genes, Bacterial Glycosyltransferases Lipopolysaccharides Pseudomonas Real-Time Polymerase Chain Reaction |
| description |
Psychrotroph microorganisms have developed cellular mechanisms to cope with cold stress. Cell envelopes are key components for bacterial survival. Outer membrane is a constituent of Gram negative bacterial envelopes, consisting of several components, such as lipopolysaccharides (LPS). In this work we investigated the relevance of envelope characteristics for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis by analyzing a mini Tn5 wapH mutant strain, encoding a core LPS glycosyltransferase. Our results showed that wapH strain is impaired to grow under low temperature but not for cold survival. The mutation in wapH, provoked a strong aggregative phenotype and modifications of envelope nanomechanical properties such as lower flexibility and higher turgor pressure, cell permeability and surface area to volume ratio (S/V). Changes in these characteristics were also observed in the wild type strain grown at different temperatures, showing higher cell flexibility but lower turgor pressure under cold conditions. Cold shock experiments indicated that an acclimation period in the wild type is necessary for cell flexibility and S/V ratio adjustments. Alteration in cell-cell interaction capabilities was observed in wapH strain. Mixed cells of wild type and wapH strains, as well as those of the wild type strain grown at different temperatures, showed a mosaic pattern of aggregation. These results indicate that wapH mutation provoked marked envelope alterations showing that LPS core conservation appears as a novel essential feature for active growth under cold conditions. © 2018 Benforte et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| title |
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis |
| title_short |
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis |
| title_full |
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis |
| title_fullStr |
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis |
| title_full_unstemmed |
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis |
| title_sort |
novel role of the lps core glycosyltransferase waph for cold adaptation in the antarctic bacterium pseudomonas extremaustralis |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v13_n2_p_Benforte http://hdl.handle.net/20.500.12110/paper_19326203_v13_n2_p_Benforte |
| _version_ |
1768543058002968576 |