Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models

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ERBB2 is a member of the ERBB family of tyrosine kinase receptors that plays a major role in breast cancer progression. Located at the plasma membrane, ERBB2 forms large clusters in spite of the presence of growth factors. Beta1 integrin, membrane receptor of extracellular matrix proteins, regulates...

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Autor principal:	Simian, Marina
Publicado:	2017
Materias:	beta1 integrin epidermal growth factor receptor 2 CD18 antigen ERBB2 protein, human Article breast cancer cell line cell adhesion cell compartmentalization cell membrane confocal microscopy enzyme localization extracellular matrix HeLa cell line human human cell immunofluorescence MCF-7 cell line molecular imaging protein analysis protein expression protein function protein interaction protein localization protein structure sequence analysis SKBR3 cell line T47D cell line Western blotting breast tumor metabolism pathology tumor cell line Breast Neoplasms CD18 Antigens Cell Line, Tumor Humans Receptor, ErbB-2
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v12_n3_p_Toscani http://hdl.handle.net/20.500.12110/paper_19326203_v12_n3_p_Toscani
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_19326203_v12_n3_p_Toscani
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spelling	paper:paper_19326203_v12_n3_p_Toscani2023-06-08T16:30:39Z Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models Simian, Marina beta1 integrin epidermal growth factor receptor 2 CD18 antigen epidermal growth factor receptor 2 ERBB2 protein, human Article breast cancer cell line cell adhesion cell compartmentalization cell membrane confocal microscopy enzyme localization extracellular matrix HeLa cell line human human cell immunofluorescence MCF-7 cell line molecular imaging protein analysis protein expression protein function protein interaction protein localization protein structure sequence analysis SKBR3 cell line T47D cell line Western blotting breast tumor metabolism pathology tumor cell line Breast Neoplasms CD18 Antigens Cell Line, Tumor Humans Receptor, ErbB-2 ERBB2 is a member of the ERBB family of tyrosine kinase receptors that plays a major role in breast cancer progression. Located at the plasma membrane, ERBB2 forms large clusters in spite of the presence of growth factors. Beta1 integrin, membrane receptor of extracellular matrix proteins, regulates adhesion, migration and invasiveness of breast cancer cells. Physical interaction between beta1 integrin and ERBB2 has been suggested although published data are contradictory. The aim of the present work was to study the interaction between ERBB2 and beta1 integrin in different scenarios of expression and activation. We determined that beta1 integrin and ERBB2 colocalization is dependent on the expression level of both receptors exclusively in adherent cells. In suspension cells, lack of focal adhesions leave integrins free to diffuse on the plasma membrane and interact with ERBB2 even at low expression levels of both receptors. In adherent cells, high expression of beta1 integrin leaves unbound receptors outside focal complexes that diffuse within the plasma membrane and interact with ERBB2 membrane domains. Superresolution imaging showed the existence of two distinct populations of ERBB2: a major population located in large clusters and a minor population outside these structures. Upon ERBB2 overexpression, receptors outside large clusters can freely diffuse at the membrane and interact with integrins. These results reveal how expression levels of beta1 integrin and ERBB2 determine their frequency of colocalization and show that extracellular matrix proteins shape membrane clusters distribution, regulating ERBB2 and beta1 integrin activity in breast cancer cells. © 2017 Toscani et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Simian, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v12_n3_p_Toscani http://hdl.handle.net/20.500.12110/paper_19326203_v12_n3_p_Toscani
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	beta1 integrin epidermal growth factor receptor 2 CD18 antigen epidermal growth factor receptor 2 ERBB2 protein, human Article breast cancer cell line cell adhesion cell compartmentalization cell membrane confocal microscopy enzyme localization extracellular matrix HeLa cell line human human cell immunofluorescence MCF-7 cell line molecular imaging protein analysis protein expression protein function protein interaction protein localization protein structure sequence analysis SKBR3 cell line T47D cell line Western blotting breast tumor metabolism pathology tumor cell line Breast Neoplasms CD18 Antigens Cell Line, Tumor Humans Receptor, ErbB-2
spellingShingle	beta1 integrin epidermal growth factor receptor 2 CD18 antigen epidermal growth factor receptor 2 ERBB2 protein, human Article breast cancer cell line cell adhesion cell compartmentalization cell membrane confocal microscopy enzyme localization extracellular matrix HeLa cell line human human cell immunofluorescence MCF-7 cell line molecular imaging protein analysis protein expression protein function protein interaction protein localization protein structure sequence analysis SKBR3 cell line T47D cell line Western blotting breast tumor metabolism pathology tumor cell line Breast Neoplasms CD18 Antigens Cell Line, Tumor Humans Receptor, ErbB-2 Simian, Marina Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
topic_facet	beta1 integrin epidermal growth factor receptor 2 CD18 antigen epidermal growth factor receptor 2 ERBB2 protein, human Article breast cancer cell line cell adhesion cell compartmentalization cell membrane confocal microscopy enzyme localization extracellular matrix HeLa cell line human human cell immunofluorescence MCF-7 cell line molecular imaging protein analysis protein expression protein function protein interaction protein localization protein structure sequence analysis SKBR3 cell line T47D cell line Western blotting breast tumor metabolism pathology tumor cell line Breast Neoplasms CD18 Antigens Cell Line, Tumor Humans Receptor, ErbB-2
description	ERBB2 is a member of the ERBB family of tyrosine kinase receptors that plays a major role in breast cancer progression. Located at the plasma membrane, ERBB2 forms large clusters in spite of the presence of growth factors. Beta1 integrin, membrane receptor of extracellular matrix proteins, regulates adhesion, migration and invasiveness of breast cancer cells. Physical interaction between beta1 integrin and ERBB2 has been suggested although published data are contradictory. The aim of the present work was to study the interaction between ERBB2 and beta1 integrin in different scenarios of expression and activation. We determined that beta1 integrin and ERBB2 colocalization is dependent on the expression level of both receptors exclusively in adherent cells. In suspension cells, lack of focal adhesions leave integrins free to diffuse on the plasma membrane and interact with ERBB2 even at low expression levels of both receptors. In adherent cells, high expression of beta1 integrin leaves unbound receptors outside focal complexes that diffuse within the plasma membrane and interact with ERBB2 membrane domains. Superresolution imaging showed the existence of two distinct populations of ERBB2: a major population located in large clusters and a minor population outside these structures. Upon ERBB2 overexpression, receptors outside large clusters can freely diffuse at the membrane and interact with integrins. These results reveal how expression levels of beta1 integrin and ERBB2 determine their frequency of colocalization and show that extracellular matrix proteins shape membrane clusters distribution, regulating ERBB2 and beta1 integrin activity in breast cancer cells. © 2017 Toscani et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
author	Simian, Marina
author_facet	Simian, Marina
author_sort	Simian, Marina
title	Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
title_short	Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
title_full	Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
title_fullStr	Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
title_full_unstemmed	Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
title_sort	distinct erbb2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v12_n3_p_Toscani http://hdl.handle.net/20.500.12110/paper_19326203_v12_n3_p_Toscani
work_keys_str_mv	AT simianmarina distincterbb2receptorpopulationsdifferentiallyinteractwithbeta1integrininbreastcancercellmodels
_version_	1768544659552862208

Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models

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