Culex pipiens development is greatly influenced by native bacteria and exogenous yeast
Culex pipiens is the most cosmopolitanmosquito of the Pipiens Assemblage. By studying the nature of interactions between this species and microorganisms common to its breeding environment we can unravel important pitfalls encountered during development.We tested the survival rate of larval stages, p...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n4_p_DiazNieto http://hdl.handle.net/20.500.12110/paper_19326203_v11_n4_p_DiazNieto |
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paper:paper_19326203_v11_n4_p_DiazNieto2023-06-08T16:30:35Z Culex pipiens development is greatly influenced by native bacteria and exogenous yeast D'Alessio, Cecilia adult adulthood Aeromonas bacterial strain breeding case report Culex pipiens diet egg laying exposure female habitat selection human Klebsiella larva larval stage mate choice microorganism molting nutritional value progeny pupa Saccharomyces cerevisiae species survival rate vertical transmission animal bacterium Culex growth, development and aging isolation and purification male microbiology microflora physiology Animals Bacteria Culex Female Larva Male Microbiota Molting Oviposition Saccharomyces cerevisiae Culex pipiens is the most cosmopolitanmosquito of the Pipiens Assemblage. By studying the nature of interactions between this species and microorganisms common to its breeding environment we can unravel important pitfalls encountered during development.We tested the survival rate of larval stages, pupae and adults of a Cx. pipiens colony exposed to a variety of microorganisms in laboratory conditions and assessed the transmission to offspring (F1) by those organisms that secured development up to adulthood. Three complementary experiments were designed to: 1) explore the nutritional value of yeasts and other microorganisms during Cx. pipiens development; 2) elucidate the transstadial transmission of yeast to the host offspring; and 3) to examine the relevance of all these microorganisms in female choice for oviposition-substratum. The yeast Saccharomyces cerevisiae proved to be the most nutritional diet, but despite showing the highest survival rates, vertical transmission to F1 was never confirmed. In addition, during the oviposition trials, none of the gravid females was attracted to the yeast substratum. Notably, the two native bacterial strains, Klebsiella sp. and Aeromonas sp., were the preferred oviposition media, the same two bacteria thatmanaged to feed neonates until molting into 2nd instar larvae. Our results not only suggest that Klebsiella sp. or Aeromonas sp. serve as attractants for oviposition habitat selection, but also nurture the most fragile instar, L1, to assure molting into a more resilient stage, L2, while yeast proves to be the most supportive diet for completing development. These experiments unearthed survival traits that might be considered in the future development of strategies of Cx. pipiens control. These studies can be extended to other members of the Pipiens Assemblage. © 2016 Díaz-Nieto et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:D'Alessio, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n4_p_DiazNieto http://hdl.handle.net/20.500.12110/paper_19326203_v11_n4_p_DiazNieto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adult adulthood Aeromonas bacterial strain breeding case report Culex pipiens diet egg laying exposure female habitat selection human Klebsiella larva larval stage mate choice microorganism molting nutritional value progeny pupa Saccharomyces cerevisiae species survival rate vertical transmission animal bacterium Culex growth, development and aging isolation and purification male microbiology microflora physiology Animals Bacteria Culex Female Larva Male Microbiota Molting Oviposition Saccharomyces cerevisiae |
| spellingShingle |
adult adulthood Aeromonas bacterial strain breeding case report Culex pipiens diet egg laying exposure female habitat selection human Klebsiella larva larval stage mate choice microorganism molting nutritional value progeny pupa Saccharomyces cerevisiae species survival rate vertical transmission animal bacterium Culex growth, development and aging isolation and purification male microbiology microflora physiology Animals Bacteria Culex Female Larva Male Microbiota Molting Oviposition Saccharomyces cerevisiae D'Alessio, Cecilia Culex pipiens development is greatly influenced by native bacteria and exogenous yeast |
| topic_facet |
adult adulthood Aeromonas bacterial strain breeding case report Culex pipiens diet egg laying exposure female habitat selection human Klebsiella larva larval stage mate choice microorganism molting nutritional value progeny pupa Saccharomyces cerevisiae species survival rate vertical transmission animal bacterium Culex growth, development and aging isolation and purification male microbiology microflora physiology Animals Bacteria Culex Female Larva Male Microbiota Molting Oviposition Saccharomyces cerevisiae |
| description |
Culex pipiens is the most cosmopolitanmosquito of the Pipiens Assemblage. By studying the nature of interactions between this species and microorganisms common to its breeding environment we can unravel important pitfalls encountered during development.We tested the survival rate of larval stages, pupae and adults of a Cx. pipiens colony exposed to a variety of microorganisms in laboratory conditions and assessed the transmission to offspring (F1) by those organisms that secured development up to adulthood. Three complementary experiments were designed to: 1) explore the nutritional value of yeasts and other microorganisms during Cx. pipiens development; 2) elucidate the transstadial transmission of yeast to the host offspring; and 3) to examine the relevance of all these microorganisms in female choice for oviposition-substratum. The yeast Saccharomyces cerevisiae proved to be the most nutritional diet, but despite showing the highest survival rates, vertical transmission to F1 was never confirmed. In addition, during the oviposition trials, none of the gravid females was attracted to the yeast substratum. Notably, the two native bacterial strains, Klebsiella sp. and Aeromonas sp., were the preferred oviposition media, the same two bacteria thatmanaged to feed neonates until molting into 2nd instar larvae. Our results not only suggest that Klebsiella sp. or Aeromonas sp. serve as attractants for oviposition habitat selection, but also nurture the most fragile instar, L1, to assure molting into a more resilient stage, L2, while yeast proves to be the most supportive diet for completing development. These experiments unearthed survival traits that might be considered in the future development of strategies of Cx. pipiens control. These studies can be extended to other members of the Pipiens Assemblage. © 2016 Díaz-Nieto et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| author |
D'Alessio, Cecilia |
| author_facet |
D'Alessio, Cecilia |
| author_sort |
D'Alessio, Cecilia |
| title |
Culex pipiens development is greatly influenced by native bacteria and exogenous yeast |
| title_short |
Culex pipiens development is greatly influenced by native bacteria and exogenous yeast |
| title_full |
Culex pipiens development is greatly influenced by native bacteria and exogenous yeast |
| title_fullStr |
Culex pipiens development is greatly influenced by native bacteria and exogenous yeast |
| title_full_unstemmed |
Culex pipiens development is greatly influenced by native bacteria and exogenous yeast |
| title_sort |
culex pipiens development is greatly influenced by native bacteria and exogenous yeast |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n4_p_DiazNieto http://hdl.handle.net/20.500.12110/paper_19326203_v11_n4_p_DiazNieto |
| work_keys_str_mv |
AT dalessiocecilia culexpipiensdevelopmentisgreatlyinfluencedbynativebacteriaandexogenousyeast |
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