Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae)
We investigated the effect of long-term starvation and posterior feeding on energetic reserves, oxidative stress, digestive enzymes, and histology of C. quadricarinatus midgut gland. The crayfish (6.27 g) were randomly assigned to one of three feeding protocols: continuous feeding throughout 80 day,...
Guardado en:
| Publicado: |
2016
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Sacristan http://hdl.handle.net/20.500.12110/paper_19326203_v11_n3_p_Sacristan |
| Aporte de: |
| id |
paper:paper_19326203_v11_n3_p_Sacristan |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_19326203_v11_n3_p_Sacristan2023-06-08T16:30:34Z Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae) catalase glutathione glycogen lipid protein proteinase thiobarbituric acid reactive substance triacylglycerol lipase catalase glutathione glycogen lipid peptide hydrolase protein triacylglycerol lipase animal structures Article biochemistry body weight Cherax quadricarinatus controlled study crayfish energy metabolism enzyme activity feeding female growth rate hepatosomatic index histology juvenile animal lipid peroxidation metabolic parameters midgut gland molting nonhuman oxidation oxidative stress physiological process starvation animal animal food chemistry enzymology growth, development and aging intestine mucosa metabolism pathology physiology Animal Feed Animals Astacoidea Catalase Glutathione Glycogen Intestinal Mucosa Lipase Lipid Peroxidation Lipids Oxidative Stress Peptide Hydrolases Proteins Starvation We investigated the effect of long-term starvation and posterior feeding on energetic reserves, oxidative stress, digestive enzymes, and histology of C. quadricarinatus midgut gland. The crayfish (6.27 g) were randomly assigned to one of three feeding protocols: continuous feeding throughout 80 day, continuous starvation until 80 day, and continuous starvation throughout 50 day and then feeding for the following 30 days. Juveniles from each protocol were weighed, and sacrificed at day 15, 30, 50 or 80. The lipids, glycogen, reduced glutathione (GSH), soluble protein, lipid peroxidation (TBARS), protein oxidation (PO), catalase (CAT), lipase and proteinase activities, and histology were measured on midgut gland. Starved crayfish had a lower hepatosomatic index, number ofmolts, specific growth rate, lipids, glycogen, and GSH levels than fed animals at all assay times. The starvation did not affect the soluble protein, TBARS, PO levels and CAT. In starved juveniles the lipase activity decreased as starvation time increased, whereas proteinase activity decreased only at day 80. The histological analysis of the starved animals showed several signs of structural alterations. After 30 days of feeding, the starved-feeding animals exhibited a striking recovery of hepatosomatic index, number of molts, lipids and glycogen, GSH, lipase activity and midgut gland structure. © 2016 Sacristán et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Sacristan http://hdl.handle.net/20.500.12110/paper_19326203_v11_n3_p_Sacristan |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
catalase glutathione glycogen lipid protein proteinase thiobarbituric acid reactive substance triacylglycerol lipase catalase glutathione glycogen lipid peptide hydrolase protein triacylglycerol lipase animal structures Article biochemistry body weight Cherax quadricarinatus controlled study crayfish energy metabolism enzyme activity feeding female growth rate hepatosomatic index histology juvenile animal lipid peroxidation metabolic parameters midgut gland molting nonhuman oxidation oxidative stress physiological process starvation animal animal food chemistry enzymology growth, development and aging intestine mucosa metabolism pathology physiology Animal Feed Animals Astacoidea Catalase Glutathione Glycogen Intestinal Mucosa Lipase Lipid Peroxidation Lipids Oxidative Stress Peptide Hydrolases Proteins Starvation |
| spellingShingle |
catalase glutathione glycogen lipid protein proteinase thiobarbituric acid reactive substance triacylglycerol lipase catalase glutathione glycogen lipid peptide hydrolase protein triacylglycerol lipase animal structures Article biochemistry body weight Cherax quadricarinatus controlled study crayfish energy metabolism enzyme activity feeding female growth rate hepatosomatic index histology juvenile animal lipid peroxidation metabolic parameters midgut gland molting nonhuman oxidation oxidative stress physiological process starvation animal animal food chemistry enzymology growth, development and aging intestine mucosa metabolism pathology physiology Animal Feed Animals Astacoidea Catalase Glutathione Glycogen Intestinal Mucosa Lipase Lipid Peroxidation Lipids Oxidative Stress Peptide Hydrolases Proteins Starvation Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae) |
| topic_facet |
catalase glutathione glycogen lipid protein proteinase thiobarbituric acid reactive substance triacylglycerol lipase catalase glutathione glycogen lipid peptide hydrolase protein triacylglycerol lipase animal structures Article biochemistry body weight Cherax quadricarinatus controlled study crayfish energy metabolism enzyme activity feeding female growth rate hepatosomatic index histology juvenile animal lipid peroxidation metabolic parameters midgut gland molting nonhuman oxidation oxidative stress physiological process starvation animal animal food chemistry enzymology growth, development and aging intestine mucosa metabolism pathology physiology Animal Feed Animals Astacoidea Catalase Glutathione Glycogen Intestinal Mucosa Lipase Lipid Peroxidation Lipids Oxidative Stress Peptide Hydrolases Proteins Starvation |
| description |
We investigated the effect of long-term starvation and posterior feeding on energetic reserves, oxidative stress, digestive enzymes, and histology of C. quadricarinatus midgut gland. The crayfish (6.27 g) were randomly assigned to one of three feeding protocols: continuous feeding throughout 80 day, continuous starvation until 80 day, and continuous starvation throughout 50 day and then feeding for the following 30 days. Juveniles from each protocol were weighed, and sacrificed at day 15, 30, 50 or 80. The lipids, glycogen, reduced glutathione (GSH), soluble protein, lipid peroxidation (TBARS), protein oxidation (PO), catalase (CAT), lipase and proteinase activities, and histology were measured on midgut gland. Starved crayfish had a lower hepatosomatic index, number ofmolts, specific growth rate, lipids, glycogen, and GSH levels than fed animals at all assay times. The starvation did not affect the soluble protein, TBARS, PO levels and CAT. In starved juveniles the lipase activity decreased as starvation time increased, whereas proteinase activity decreased only at day 80. The histological analysis of the starved animals showed several signs of structural alterations. After 30 days of feeding, the starved-feeding animals exhibited a striking recovery of hepatosomatic index, number of molts, lipids and glycogen, GSH, lipase activity and midgut gland structure. © 2016 Sacristán et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| title |
Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae) |
| title_short |
Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae) |
| title_full |
Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae) |
| title_fullStr |
Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae) |
| title_full_unstemmed |
Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae) |
| title_sort |
long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of cherax quadricarinatus juveniles (parastacidae) |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Sacristan http://hdl.handle.net/20.500.12110/paper_19326203_v11_n3_p_Sacristan |
| _version_ |
1768544659368312832 |