Fluctuations, correlations and the estimation of concentrations inside cells
Information transmission in cells occurs quite accurately even when concentration changes are "read" by individual binding sites. In this paper we study ligand number and site occupancy fluctuations when ligands diffuse and react going beyond the analyses that focus on their asymptotic dec...
Guardado en:
| Publicado: |
2016
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Ipina http://hdl.handle.net/20.500.12110/paper_19326203_v11_n3_p_Ipina |
| Aporte de: |
| id |
paper:paper_19326203_v11_n3_p_Ipina |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_19326203_v11_n3_p_Ipina2023-06-08T16:30:34Z Fluctuations, correlations and the estimation of concentrations inside cells Article binding site cell level concentration (parameters) controlled study enzyme activity ligand binding mathematical computing molecular dynamics process model stochastic model animal biological model biosynthesis Drosophila melanogaster genetic transcription physiology messenger RNA Animals Drosophila melanogaster Models, Biological RNA, Messenger Transcription, Genetic Information transmission in cells occurs quite accurately even when concentration changes are "read" by individual binding sites. In this paper we study ligand number and site occupancy fluctuations when ligands diffuse and react going beyond the analyses that focus on their asymptotic decay. In this way we show that, for immobile binding sites, fluctuations in the number of bound molecules decay on a relatively fast scale before the asymptotic behavior kicks in. This result can explain the observed co-existence of highly fluctuating instantaneous transcriptional activities with accumulated mRNA concentrations that have relatively small noise levels. We also show that the initial stages of the decay in the bound molecule number fluctuations have one or two characteristic timescales depending on the concentration of free molecules. This transition can explain the changes in enzyme activity observed at the single molecule level. © 2016 Pérez Ipiña, Ponce Dawson. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Ipina http://hdl.handle.net/20.500.12110/paper_19326203_v11_n3_p_Ipina |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Article binding site cell level concentration (parameters) controlled study enzyme activity ligand binding mathematical computing molecular dynamics process model stochastic model animal biological model biosynthesis Drosophila melanogaster genetic transcription physiology messenger RNA Animals Drosophila melanogaster Models, Biological RNA, Messenger Transcription, Genetic |
| spellingShingle |
Article binding site cell level concentration (parameters) controlled study enzyme activity ligand binding mathematical computing molecular dynamics process model stochastic model animal biological model biosynthesis Drosophila melanogaster genetic transcription physiology messenger RNA Animals Drosophila melanogaster Models, Biological RNA, Messenger Transcription, Genetic Fluctuations, correlations and the estimation of concentrations inside cells |
| topic_facet |
Article binding site cell level concentration (parameters) controlled study enzyme activity ligand binding mathematical computing molecular dynamics process model stochastic model animal biological model biosynthesis Drosophila melanogaster genetic transcription physiology messenger RNA Animals Drosophila melanogaster Models, Biological RNA, Messenger Transcription, Genetic |
| description |
Information transmission in cells occurs quite accurately even when concentration changes are "read" by individual binding sites. In this paper we study ligand number and site occupancy fluctuations when ligands diffuse and react going beyond the analyses that focus on their asymptotic decay. In this way we show that, for immobile binding sites, fluctuations in the number of bound molecules decay on a relatively fast scale before the asymptotic behavior kicks in. This result can explain the observed co-existence of highly fluctuating instantaneous transcriptional activities with accumulated mRNA concentrations that have relatively small noise levels. We also show that the initial stages of the decay in the bound molecule number fluctuations have one or two characteristic timescales depending on the concentration of free molecules. This transition can explain the changes in enzyme activity observed at the single molecule level. © 2016 Pérez Ipiña, Ponce Dawson. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| title |
Fluctuations, correlations and the estimation of concentrations inside cells |
| title_short |
Fluctuations, correlations and the estimation of concentrations inside cells |
| title_full |
Fluctuations, correlations and the estimation of concentrations inside cells |
| title_fullStr |
Fluctuations, correlations and the estimation of concentrations inside cells |
| title_full_unstemmed |
Fluctuations, correlations and the estimation of concentrations inside cells |
| title_sort |
fluctuations, correlations and the estimation of concentrations inside cells |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Ipina http://hdl.handle.net/20.500.12110/paper_19326203_v11_n3_p_Ipina |
| _version_ |
1768542572326682624 |