More Cercospora species infect soybeans across the Americas than meets the eye
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Ce...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n8_p_Soares http://hdl.handle.net/20.500.12110/paper_19326203_v10_n8_p_Soares |
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paper:paper_19326203_v10_n8_p_Soares2023-06-08T16:30:31Z More Cercospora species infect soybeans across the Americas than meets the eye Guillin, Eduardo Alejandro amino acid substitution antifungal resistance Argentina Article Brazil Cercospora Cercospora kikuchii Cercospora sojina cfp gene fungal gene fungal plant disease fungal strain fungus culture gene sequence genetic analysis genetic recombination genetic variability haplotype mitochondrial gene monophyly nonhuman phylogeny phylogeography soybean tub gene Ascomycetes Bayes theorem gene regulatory network genetic variation genetics isolation and purification Japan microbiology physiology plant disease plant leaf plant seed soybean Western Hemisphere mitochondrial DNA Americas Ascomycota Bayes Theorem DNA, Mitochondrial Gene Regulatory Networks Genes, Fungal Genetic Variation Haplotypes Japan Phylogeny Plant Diseases Plant Leaves Recombination, Genetic Seeds Soybeans Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora. Copyright: © 2015 Soares et al. Fil:Guillin, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n8_p_Soares http://hdl.handle.net/20.500.12110/paper_19326203_v10_n8_p_Soares |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
amino acid substitution antifungal resistance Argentina Article Brazil Cercospora Cercospora kikuchii Cercospora sojina cfp gene fungal gene fungal plant disease fungal strain fungus culture gene sequence genetic analysis genetic recombination genetic variability haplotype mitochondrial gene monophyly nonhuman phylogeny phylogeography soybean tub gene Ascomycetes Bayes theorem gene regulatory network genetic variation genetics isolation and purification Japan microbiology physiology plant disease plant leaf plant seed soybean Western Hemisphere mitochondrial DNA Americas Ascomycota Bayes Theorem DNA, Mitochondrial Gene Regulatory Networks Genes, Fungal Genetic Variation Haplotypes Japan Phylogeny Plant Diseases Plant Leaves Recombination, Genetic Seeds Soybeans |
| spellingShingle |
amino acid substitution antifungal resistance Argentina Article Brazil Cercospora Cercospora kikuchii Cercospora sojina cfp gene fungal gene fungal plant disease fungal strain fungus culture gene sequence genetic analysis genetic recombination genetic variability haplotype mitochondrial gene monophyly nonhuman phylogeny phylogeography soybean tub gene Ascomycetes Bayes theorem gene regulatory network genetic variation genetics isolation and purification Japan microbiology physiology plant disease plant leaf plant seed soybean Western Hemisphere mitochondrial DNA Americas Ascomycota Bayes Theorem DNA, Mitochondrial Gene Regulatory Networks Genes, Fungal Genetic Variation Haplotypes Japan Phylogeny Plant Diseases Plant Leaves Recombination, Genetic Seeds Soybeans Guillin, Eduardo Alejandro More Cercospora species infect soybeans across the Americas than meets the eye |
| topic_facet |
amino acid substitution antifungal resistance Argentina Article Brazil Cercospora Cercospora kikuchii Cercospora sojina cfp gene fungal gene fungal plant disease fungal strain fungus culture gene sequence genetic analysis genetic recombination genetic variability haplotype mitochondrial gene monophyly nonhuman phylogeny phylogeography soybean tub gene Ascomycetes Bayes theorem gene regulatory network genetic variation genetics isolation and purification Japan microbiology physiology plant disease plant leaf plant seed soybean Western Hemisphere mitochondrial DNA Americas Ascomycota Bayes Theorem DNA, Mitochondrial Gene Regulatory Networks Genes, Fungal Genetic Variation Haplotypes Japan Phylogeny Plant Diseases Plant Leaves Recombination, Genetic Seeds Soybeans |
| description |
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora. Copyright: © 2015 Soares et al. |
| author |
Guillin, Eduardo Alejandro |
| author_facet |
Guillin, Eduardo Alejandro |
| author_sort |
Guillin, Eduardo Alejandro |
| title |
More Cercospora species infect soybeans across the Americas than meets the eye |
| title_short |
More Cercospora species infect soybeans across the Americas than meets the eye |
| title_full |
More Cercospora species infect soybeans across the Americas than meets the eye |
| title_fullStr |
More Cercospora species infect soybeans across the Americas than meets the eye |
| title_full_unstemmed |
More Cercospora species infect soybeans across the Americas than meets the eye |
| title_sort |
more cercospora species infect soybeans across the americas than meets the eye |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n8_p_Soares http://hdl.handle.net/20.500.12110/paper_19326203_v10_n8_p_Soares |
| work_keys_str_mv |
AT guillineduardoalejandro morecercosporaspeciesinfectsoybeansacrosstheamericasthanmeetstheeye |
| _version_ |
1768542572144230400 |