Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata
We studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or fe...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n3_p_Paladino http://hdl.handle.net/20.500.12110/paper_19326203_v10_n3_p_Paladino |
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paper:paper_19326203_v10_n3_p_Paladino2023-06-08T16:30:28Z Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata Lanzavecchia, Silvia Beatriz Viscarret, Mariana Mabel Papeschi, Alba Graciela Cladera, Jorge Luis Bressa, Maria Jose adult Article cell structure controlled study Diachasmimorpha longicaudata diploidy female gene structure haploidy hemizygote heterozygote male Mediterranean fruit fly nonhuman phylogeny progeny sex determination process sex ratio spermatid spermatocyte spermatozoon wasp allele animal evolution genetics physiology sex determination process wasp Diachasmimorpha longicaudata Hexapoda Hymenoptera Tephritidae Vespoidea Alleles Animals Biological Evolution Diploidy Female Haploidy Male Sex Determination Processes Sex Ratio Wasps We studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or female development depends on the allelic composition of one sex locus (single-locus CSD) or multiple sex loci (multiple-locus CSD). Hemizygote individuals are normal haploid males, and heterozygotes for at least one sex locus are normal diploid females, but homozygotes for all the sex loci are diploid males. In order to force the occurrence of diploid males in D. longicaudata, we established highly inbred lines and examined their offspring using chromosome counting, flow cytometry, and sex ratio analysis. We found that when mother-son crosses were studied, this wasp produced about 20% of diploid males out of the total male progeny. Our results suggest that this parasitoid may represent the second genus with multiple-locus CSD in Hymenoptera. Knowledge about the sex determination system in D. longicaudata is relevant for the improvement of mass rearing protocols of this species. This information also provides the necessary background for further investigations on the underlying molecular mechanisms of sex determination in this species, and a better insight into the evolution of this pathway in Hymenoptera in particular and insects in general. © 2015 Carabajal Paladino et al. Fil:Lanzavecchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Viscarret, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Papeschi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cladera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bressa, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n3_p_Paladino http://hdl.handle.net/20.500.12110/paper_19326203_v10_n3_p_Paladino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adult Article cell structure controlled study Diachasmimorpha longicaudata diploidy female gene structure haploidy hemizygote heterozygote male Mediterranean fruit fly nonhuman phylogeny progeny sex determination process sex ratio spermatid spermatocyte spermatozoon wasp allele animal evolution genetics physiology sex determination process wasp Diachasmimorpha longicaudata Hexapoda Hymenoptera Tephritidae Vespoidea Alleles Animals Biological Evolution Diploidy Female Haploidy Male Sex Determination Processes Sex Ratio Wasps |
| spellingShingle |
adult Article cell structure controlled study Diachasmimorpha longicaudata diploidy female gene structure haploidy hemizygote heterozygote male Mediterranean fruit fly nonhuman phylogeny progeny sex determination process sex ratio spermatid spermatocyte spermatozoon wasp allele animal evolution genetics physiology sex determination process wasp Diachasmimorpha longicaudata Hexapoda Hymenoptera Tephritidae Vespoidea Alleles Animals Biological Evolution Diploidy Female Haploidy Male Sex Determination Processes Sex Ratio Wasps Lanzavecchia, Silvia Beatriz Viscarret, Mariana Mabel Papeschi, Alba Graciela Cladera, Jorge Luis Bressa, Maria Jose Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata |
| topic_facet |
adult Article cell structure controlled study Diachasmimorpha longicaudata diploidy female gene structure haploidy hemizygote heterozygote male Mediterranean fruit fly nonhuman phylogeny progeny sex determination process sex ratio spermatid spermatocyte spermatozoon wasp allele animal evolution genetics physiology sex determination process wasp Diachasmimorpha longicaudata Hexapoda Hymenoptera Tephritidae Vespoidea Alleles Animals Biological Evolution Diploidy Female Haploidy Male Sex Determination Processes Sex Ratio Wasps |
| description |
We studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or female development depends on the allelic composition of one sex locus (single-locus CSD) or multiple sex loci (multiple-locus CSD). Hemizygote individuals are normal haploid males, and heterozygotes for at least one sex locus are normal diploid females, but homozygotes for all the sex loci are diploid males. In order to force the occurrence of diploid males in D. longicaudata, we established highly inbred lines and examined their offspring using chromosome counting, flow cytometry, and sex ratio analysis. We found that when mother-son crosses were studied, this wasp produced about 20% of diploid males out of the total male progeny. Our results suggest that this parasitoid may represent the second genus with multiple-locus CSD in Hymenoptera. Knowledge about the sex determination system in D. longicaudata is relevant for the improvement of mass rearing protocols of this species. This information also provides the necessary background for further investigations on the underlying molecular mechanisms of sex determination in this species, and a better insight into the evolution of this pathway in Hymenoptera in particular and insects in general. © 2015 Carabajal Paladino et al. |
| author |
Lanzavecchia, Silvia Beatriz Viscarret, Mariana Mabel Papeschi, Alba Graciela Cladera, Jorge Luis Bressa, Maria Jose |
| author_facet |
Lanzavecchia, Silvia Beatriz Viscarret, Mariana Mabel Papeschi, Alba Graciela Cladera, Jorge Luis Bressa, Maria Jose |
| author_sort |
Lanzavecchia, Silvia Beatriz |
| title |
Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata |
| title_short |
Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata |
| title_full |
Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata |
| title_fullStr |
Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata |
| title_full_unstemmed |
Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata |
| title_sort |
complementary sex determination in the parasitic wasp diachasmimorpha longicaudata |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n3_p_Paladino http://hdl.handle.net/20.500.12110/paper_19326203_v10_n3_p_Paladino |
| work_keys_str_mv |
AT lanzavecchiasilviabeatriz complementarysexdeterminationintheparasiticwaspdiachasmimorphalongicaudata AT viscarretmarianamabel complementarysexdeterminationintheparasiticwaspdiachasmimorphalongicaudata AT papeschialbagraciela complementarysexdeterminationintheparasiticwaspdiachasmimorphalongicaudata AT claderajorgeluis complementarysexdeterminationintheparasiticwaspdiachasmimorphalongicaudata AT bressamariajose complementarysexdeterminationintheparasiticwaspdiachasmimorphalongicaudata |
| _version_ |
1768544431401598976 |