Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell...

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Autores principales:	Questa, María, Romorini, Leonardo, Luzzani, Carlos Daniel, Scassa, María Elida, Guberman, Alejandra Sonia
Publicado:	2016
Materias:	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18735061_v16_n2_p300_Questa http://hdl.handle.net/20.500.12110/paper_18735061_v16_n2_p300_Questa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_18735061_v16_n2_p300_Questa
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spelling	paper:paper_18735061_v16_n2_p300_Questa2023-06-08T16:30:01Z Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts Questa, María Romorini, Leonardo Luzzani, Carlos Daniel Scassa, María Elida Guberman, Alejandra Sonia kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. © 2015 The Authors. Fil:Questa, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romorini, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scassa, M.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18735061_v16_n2_p300_Questa http://hdl.handle.net/20.500.12110/paper_18735061_v16_n2_p300_Questa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors
spellingShingle	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors Questa, María Romorini, Leonardo Luzzani, Carlos Daniel Scassa, María Elida Guberman, Alejandra Sonia Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
topic_facet	kruppel like factor 4 Myc protein octamer transcription factor 4 transcription factor Sox2 octamer transcription factor 4 POU5F1 protein, human transcription factor Article cell differentiation controlled study CpG island fibroblast gene expression human human cell human embryonic stem cell hypoxic cell in vitro study in vivo study nuclear reprogramming pluripotent stem cell prepuce priority journal stem cell line cell culture comparative genomic hybridization cytology DNA methylation fibroblast fluorescence microscopy genetics induced pluripotent stem cell karyotype male metabolism prepuce promoter region real time polymerase chain reaction Cell Differentiation Cells, Cultured Cellular Reprogramming Comparative Genomic Hybridization DNA Methylation Fibroblasts Foreskin Humans Induced Pluripotent Stem Cells Karyotype Male Microscopy, Fluorescence Octamer Transcription Factor-3 Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Transcription Factors
description	Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. © 2015 The Authors.
author	Questa, María Romorini, Leonardo Luzzani, Carlos Daniel Scassa, María Elida Guberman, Alejandra Sonia
author_facet	Questa, María Romorini, Leonardo Luzzani, Carlos Daniel Scassa, María Elida Guberman, Alejandra Sonia
author_sort	Questa, María
title	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_short	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_full	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_fullStr	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_full_unstemmed	Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
title_sort	generation of ipsc line ipsc-fh2.1 in hypoxic conditions from human foreskin fibroblasts
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18735061_v16_n2_p300_Questa http://hdl.handle.net/20.500.12110/paper_18735061_v16_n2_p300_Questa
work_keys_str_mv	AT questamaria generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT romorinileonardo generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT luzzanicarlosdaniel generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT scassamariaelida generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT gubermanalejandrasonia generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts
_version_	1768542055113424896

Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

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