Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons
Leptin is an adipose-derived hormone that controls appetite and energy expenditure. Leptin receptors are expressed on extra-hypothalamic ventrobasal (VB) and reticular thalamic (RTN) nuclei from embryonic stages. Here, we studied the effects of pressure-puff, local application of leptin on both syna...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18632653_v223_n5_p2499_Perissinotti http://hdl.handle.net/20.500.12110/paper_18632653_v223_n5_p2499_Perissinotti |
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paper:paper_18632653_v223_n5_p2499_Perissinotti2023-06-08T16:29:34Z Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons GABA Leptin Mouse ob/ob Thalamic reticular nucleus 4 aminobutyric acid calcium activated potassium channel glutamic acid Janus kinase 2 leptin recombinant leptin 4 aminobutyric acid 6 cyano 7 nitro 2,3 quinoxalinedione alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide amino acid receptor blocking agent enzyme inhibitor Jak2 protein, mouse Janus kinase 2 leptin sodium channel blocking agent tetrodotoxin tyrphostin 4 aminobutyric acid release action potential animal experiment animal tissue Article brain slice controlled study GABAergic transmission glutamatergic synapse male mouse nerve excitability neuromodulation nonhuman obesity priority journal synaptic transmission thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp animal body temperature C57BL mouse cytology deficiency drug effect genetics metabolism nerve cell physiology signal transduction synaptic potential thalamus nucleus transgenic mouse 6-Cyano-7-nitroquinoxaline-2,3-dione Action Potentials Animals Body Temperature Enzyme Inhibitors Excitatory Amino Acid Antagonists gamma-Aminobutyric Acid Janus Kinase 2 Leptin Male Mice Mice, Inbred C57BL Mice, Transgenic Neurons Signal Transduction Sodium Channel Blockers Synaptic Potentials Synaptic Transmission Tetrodotoxin Thalamic Nuclei Tyrphostins Leptin is an adipose-derived hormone that controls appetite and energy expenditure. Leptin receptors are expressed on extra-hypothalamic ventrobasal (VB) and reticular thalamic (RTN) nuclei from embryonic stages. Here, we studied the effects of pressure-puff, local application of leptin on both synaptic transmission and action potential properties of thalamic neurons in thalamocortical slices. We used whole-cell patch-clamp recordings of thalamocortical VB neurons from wild-type (WT) and leptin-deficient obese (ob/ob) mice. We observed differences in VB neurons action potentials and synaptic currents kinetics when comparing WT vs. ob/ob. Leptin reduced GABA release onto VB neurons throughout the activation of a JAK2-dependent pathway, without affecting excitatory glutamate transmission. We observed a rapid and reversible reduction by leptin of the number of action potentials of VB neurons via the activation of large conductance Ca 2+ -dependent potassium channels. These leptin effects were observed in thalamocortical slices from up to 5-week-old WT but not in leptin-deficient obese mice. Results described here suggest the existence of a leptin-mediated trophic modulation of thalamocortical excitability during postnatal development. These findings could contribute to a better understanding of leptin within the thalamocortical system and sleep deficits in obesity. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18632653_v223_n5_p2499_Perissinotti http://hdl.handle.net/20.500.12110/paper_18632653_v223_n5_p2499_Perissinotti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
GABA Leptin Mouse ob/ob Thalamic reticular nucleus 4 aminobutyric acid calcium activated potassium channel glutamic acid Janus kinase 2 leptin recombinant leptin 4 aminobutyric acid 6 cyano 7 nitro 2,3 quinoxalinedione alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide amino acid receptor blocking agent enzyme inhibitor Jak2 protein, mouse Janus kinase 2 leptin sodium channel blocking agent tetrodotoxin tyrphostin 4 aminobutyric acid release action potential animal experiment animal tissue Article brain slice controlled study GABAergic transmission glutamatergic synapse male mouse nerve excitability neuromodulation nonhuman obesity priority journal synaptic transmission thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp animal body temperature C57BL mouse cytology deficiency drug effect genetics metabolism nerve cell physiology signal transduction synaptic potential thalamus nucleus transgenic mouse 6-Cyano-7-nitroquinoxaline-2,3-dione Action Potentials Animals Body Temperature Enzyme Inhibitors Excitatory Amino Acid Antagonists gamma-Aminobutyric Acid Janus Kinase 2 Leptin Male Mice Mice, Inbred C57BL Mice, Transgenic Neurons Signal Transduction Sodium Channel Blockers Synaptic Potentials Synaptic Transmission Tetrodotoxin Thalamic Nuclei Tyrphostins |
| spellingShingle |
GABA Leptin Mouse ob/ob Thalamic reticular nucleus 4 aminobutyric acid calcium activated potassium channel glutamic acid Janus kinase 2 leptin recombinant leptin 4 aminobutyric acid 6 cyano 7 nitro 2,3 quinoxalinedione alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide amino acid receptor blocking agent enzyme inhibitor Jak2 protein, mouse Janus kinase 2 leptin sodium channel blocking agent tetrodotoxin tyrphostin 4 aminobutyric acid release action potential animal experiment animal tissue Article brain slice controlled study GABAergic transmission glutamatergic synapse male mouse nerve excitability neuromodulation nonhuman obesity priority journal synaptic transmission thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp animal body temperature C57BL mouse cytology deficiency drug effect genetics metabolism nerve cell physiology signal transduction synaptic potential thalamus nucleus transgenic mouse 6-Cyano-7-nitroquinoxaline-2,3-dione Action Potentials Animals Body Temperature Enzyme Inhibitors Excitatory Amino Acid Antagonists gamma-Aminobutyric Acid Janus Kinase 2 Leptin Male Mice Mice, Inbred C57BL Mice, Transgenic Neurons Signal Transduction Sodium Channel Blockers Synaptic Potentials Synaptic Transmission Tetrodotoxin Thalamic Nuclei Tyrphostins Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
| topic_facet |
GABA Leptin Mouse ob/ob Thalamic reticular nucleus 4 aminobutyric acid calcium activated potassium channel glutamic acid Janus kinase 2 leptin recombinant leptin 4 aminobutyric acid 6 cyano 7 nitro 2,3 quinoxalinedione alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide amino acid receptor blocking agent enzyme inhibitor Jak2 protein, mouse Janus kinase 2 leptin sodium channel blocking agent tetrodotoxin tyrphostin 4 aminobutyric acid release action potential animal experiment animal tissue Article brain slice controlled study GABAergic transmission glutamatergic synapse male mouse nerve excitability neuromodulation nonhuman obesity priority journal synaptic transmission thalamus reticular nucleus thalamus ventral nucleus whole cell patch clamp animal body temperature C57BL mouse cytology deficiency drug effect genetics metabolism nerve cell physiology signal transduction synaptic potential thalamus nucleus transgenic mouse 6-Cyano-7-nitroquinoxaline-2,3-dione Action Potentials Animals Body Temperature Enzyme Inhibitors Excitatory Amino Acid Antagonists gamma-Aminobutyric Acid Janus Kinase 2 Leptin Male Mice Mice, Inbred C57BL Mice, Transgenic Neurons Signal Transduction Sodium Channel Blockers Synaptic Potentials Synaptic Transmission Tetrodotoxin Thalamic Nuclei Tyrphostins |
| description |
Leptin is an adipose-derived hormone that controls appetite and energy expenditure. Leptin receptors are expressed on extra-hypothalamic ventrobasal (VB) and reticular thalamic (RTN) nuclei from embryonic stages. Here, we studied the effects of pressure-puff, local application of leptin on both synaptic transmission and action potential properties of thalamic neurons in thalamocortical slices. We used whole-cell patch-clamp recordings of thalamocortical VB neurons from wild-type (WT) and leptin-deficient obese (ob/ob) mice. We observed differences in VB neurons action potentials and synaptic currents kinetics when comparing WT vs. ob/ob. Leptin reduced GABA release onto VB neurons throughout the activation of a JAK2-dependent pathway, without affecting excitatory glutamate transmission. We observed a rapid and reversible reduction by leptin of the number of action potentials of VB neurons via the activation of large conductance Ca 2+ -dependent potassium channels. These leptin effects were observed in thalamocortical slices from up to 5-week-old WT but not in leptin-deficient obese mice. Results described here suggest the existence of a leptin-mediated trophic modulation of thalamocortical excitability during postnatal development. These findings could contribute to a better understanding of leptin within the thalamocortical system and sleep deficits in obesity. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. |
| title |
Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
| title_short |
Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
| title_full |
Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
| title_fullStr |
Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
| title_full_unstemmed |
Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
| title_sort |
leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18632653_v223_n5_p2499_Perissinotti http://hdl.handle.net/20.500.12110/paper_18632653_v223_n5_p2499_Perissinotti |
| _version_ |
1768543440385081344 |