Involvement of cellular proteins in Junin arenavirus entry

Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the...

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Publicado:	2009
Materias:	Arenavirus Endocytosis Junin virus Actin cytoskeleton Cellular proteins Clathrin Dynamin Endocytic pathways Human pathogens Late endosomes Microtubules PH-dependent Vero cells cell protein chlorpromazine dynamin II epidermal growth factor receptor pathway substrate 15 Rab protein Rab7 protein virus nucleoprotein animal cell article controlled study embryo endocytosis endosome genetic transfection human human cell immunofluorescence test mouse nonhuman priority journal protein analysis protein function signal transduction Vero cell virus cell interaction virus entry virus replication Animals Calcium-Binding Proteins Cercopithecus aethiops Dynamin II Humans Intracellular Signaling Peptides and Proteins Mice Phosphoproteins rab GTP-Binding Proteins rab5 GTP-Binding Proteins Vero Cells Viral Load Viral Proteins Virus Internalization
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18606768_v4_n6_p866_Martinez http://hdl.handle.net/20.500.12110/paper_18606768_v4_n6_p866_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_18606768_v4_n6_p866_Martinez
record_format	dspace
spelling	paper:paper_18606768_v4_n6_p866_Martinez2023-06-08T16:29:28Z Involvement of cellular proteins in Junin arenavirus entry Arenavirus Endocytosis Junin virus Actin cytoskeleton Arenavirus Cellular proteins Clathrin Dynamin Endocytic pathways Endocytosis Human pathogens Junin virus Late endosomes Microtubules PH-dependent Vero cells cell protein chlorpromazine dynamin II epidermal growth factor receptor pathway substrate 15 Rab protein Rab7 protein virus nucleoprotein animal cell article controlled study embryo endocytosis endosome genetic transfection human human cell immunofluorescence test Junin virus mouse nonhuman priority journal protein analysis protein function signal transduction Vero cell virus cell interaction virus entry virus replication Animals Calcium-Binding Proteins Cercopithecus aethiops Dynamin II Endocytosis Humans Intracellular Signaling Peptides and Proteins Junin virus Mice Phosphoproteins rab GTP-Binding Proteins rab5 GTP-Binding Proteins Vero Cells Viral Load Viral Proteins Virus Internalization Arenavirus Junin virus Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18606768_v4_n6_p866_Martinez http://hdl.handle.net/20.500.12110/paper_18606768_v4_n6_p866_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arenavirus Endocytosis Junin virus Actin cytoskeleton Arenavirus Cellular proteins Clathrin Dynamin Endocytic pathways Endocytosis Human pathogens Junin virus Late endosomes Microtubules PH-dependent Vero cells cell protein chlorpromazine dynamin II epidermal growth factor receptor pathway substrate 15 Rab protein Rab7 protein virus nucleoprotein animal cell article controlled study embryo endocytosis endosome genetic transfection human human cell immunofluorescence test Junin virus mouse nonhuman priority journal protein analysis protein function signal transduction Vero cell virus cell interaction virus entry virus replication Animals Calcium-Binding Proteins Cercopithecus aethiops Dynamin II Endocytosis Humans Intracellular Signaling Peptides and Proteins Junin virus Mice Phosphoproteins rab GTP-Binding Proteins rab5 GTP-Binding Proteins Vero Cells Viral Load Viral Proteins Virus Internalization Arenavirus Junin virus
spellingShingle	Arenavirus Endocytosis Junin virus Actin cytoskeleton Arenavirus Cellular proteins Clathrin Dynamin Endocytic pathways Endocytosis Human pathogens Junin virus Late endosomes Microtubules PH-dependent Vero cells cell protein chlorpromazine dynamin II epidermal growth factor receptor pathway substrate 15 Rab protein Rab7 protein virus nucleoprotein animal cell article controlled study embryo endocytosis endosome genetic transfection human human cell immunofluorescence test Junin virus mouse nonhuman priority journal protein analysis protein function signal transduction Vero cell virus cell interaction virus entry virus replication Animals Calcium-Binding Proteins Cercopithecus aethiops Dynamin II Endocytosis Humans Intracellular Signaling Peptides and Proteins Junin virus Mice Phosphoproteins rab GTP-Binding Proteins rab5 GTP-Binding Proteins Vero Cells Viral Load Viral Proteins Virus Internalization Arenavirus Junin virus Involvement of cellular proteins in Junin arenavirus entry
topic_facet	Arenavirus Endocytosis Junin virus Actin cytoskeleton Arenavirus Cellular proteins Clathrin Dynamin Endocytic pathways Endocytosis Human pathogens Junin virus Late endosomes Microtubules PH-dependent Vero cells cell protein chlorpromazine dynamin II epidermal growth factor receptor pathway substrate 15 Rab protein Rab7 protein virus nucleoprotein animal cell article controlled study embryo endocytosis endosome genetic transfection human human cell immunofluorescence test Junin virus mouse nonhuman priority journal protein analysis protein function signal transduction Vero cell virus cell interaction virus entry virus replication Animals Calcium-Binding Proteins Cercopithecus aethiops Dynamin II Endocytosis Humans Intracellular Signaling Peptides and Proteins Junin virus Mice Phosphoproteins rab GTP-Binding Proteins rab5 GTP-Binding Proteins Vero Cells Viral Load Viral Proteins Virus Internalization Arenavirus Junin virus
description	Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
title	Involvement of cellular proteins in Junin arenavirus entry
title_short	Involvement of cellular proteins in Junin arenavirus entry
title_full	Involvement of cellular proteins in Junin arenavirus entry
title_fullStr	Involvement of cellular proteins in Junin arenavirus entry
title_full_unstemmed	Involvement of cellular proteins in Junin arenavirus entry
title_sort	involvement of cellular proteins in junin arenavirus entry
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18606768_v4_n6_p866_Martinez http://hdl.handle.net/20.500.12110/paper_18606768_v4_n6_p866_Martinez
_version_	1768545437931798528

Involvement of cellular proteins in Junin arenavirus entry

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