Diversity of small RNAs expressed in Pseudomonas species
RNA sequencing (RNA-seq) has revealed several hundreds of previously undetected small RNAs (sRNAs) in all bacterial species investigated, including strains of Pseudomonas aeruginosa, Pseudomonas putida and Pseudomonas syringae. Nonetheless, only little is known about the extent of conservation of ex...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17582229_v7_n2_p227_GomezLozano http://hdl.handle.net/20.500.12110/paper_17582229_v7_n2_p227_GomezLozano |
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paper:paper_17582229_v7_n2_p227_GomezLozano2023-06-08T16:29:00Z Diversity of small RNAs expressed in Pseudomonas species Tribelli, Paula María Bacteria (microorganisms) Pseudomonas Pseudomonas aeruginosa Pseudomonas putida Pseudomonas syringae bacterial RNA small untranslated RNA classification conserved sequence gene expression profiling genetic variation genetics Pseudomonas sequence homology Conserved Sequence Gene Expression Profiling Genetic Variation Pseudomonas RNA, Bacterial RNA, Small Untranslated Sequence Homology, Nucleic Acid RNA sequencing (RNA-seq) has revealed several hundreds of previously undetected small RNAs (sRNAs) in all bacterial species investigated, including strains of Pseudomonas aeruginosa, Pseudomonas putida and Pseudomonas syringae. Nonetheless, only little is known about the extent of conservation of expressed sRNAs across strains and species. In this study, we have used RNA-seq to identify sRNAs in P.putidaDOT-T1E and Pseudomonas extremaustralis 14-3b. This is the first strain of P.extremaustralis and the second strain of P.putida to have their transcriptomes analysed for sRNAs, and we identify the presence of around 150 novel sRNAs in each strain. Furthermore, we provide a comparison based on sequence conservation of all the sRNAs detected by RNA-seq in the Pseudomonas species investigated so far. Our results show that the extent of sRNA conservation across different species is very limited. In addition, when comparing the sRNAs expressed in different strains of the same species, we observe that numerous sRNAs exhibit a strain-specific expression pattern. These results support the idea that the evolution of most bacterial sRNAs is rapid, which limits the extent of both interspecies and intraspecies conservation. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17582229_v7_n2_p227_GomezLozano http://hdl.handle.net/20.500.12110/paper_17582229_v7_n2_p227_GomezLozano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacteria (microorganisms) Pseudomonas Pseudomonas aeruginosa Pseudomonas putida Pseudomonas syringae bacterial RNA small untranslated RNA classification conserved sequence gene expression profiling genetic variation genetics Pseudomonas sequence homology Conserved Sequence Gene Expression Profiling Genetic Variation Pseudomonas RNA, Bacterial RNA, Small Untranslated Sequence Homology, Nucleic Acid |
| spellingShingle |
Bacteria (microorganisms) Pseudomonas Pseudomonas aeruginosa Pseudomonas putida Pseudomonas syringae bacterial RNA small untranslated RNA classification conserved sequence gene expression profiling genetic variation genetics Pseudomonas sequence homology Conserved Sequence Gene Expression Profiling Genetic Variation Pseudomonas RNA, Bacterial RNA, Small Untranslated Sequence Homology, Nucleic Acid Tribelli, Paula María Diversity of small RNAs expressed in Pseudomonas species |
| topic_facet |
Bacteria (microorganisms) Pseudomonas Pseudomonas aeruginosa Pseudomonas putida Pseudomonas syringae bacterial RNA small untranslated RNA classification conserved sequence gene expression profiling genetic variation genetics Pseudomonas sequence homology Conserved Sequence Gene Expression Profiling Genetic Variation Pseudomonas RNA, Bacterial RNA, Small Untranslated Sequence Homology, Nucleic Acid |
| description |
RNA sequencing (RNA-seq) has revealed several hundreds of previously undetected small RNAs (sRNAs) in all bacterial species investigated, including strains of Pseudomonas aeruginosa, Pseudomonas putida and Pseudomonas syringae. Nonetheless, only little is known about the extent of conservation of expressed sRNAs across strains and species. In this study, we have used RNA-seq to identify sRNAs in P.putidaDOT-T1E and Pseudomonas extremaustralis 14-3b. This is the first strain of P.extremaustralis and the second strain of P.putida to have their transcriptomes analysed for sRNAs, and we identify the presence of around 150 novel sRNAs in each strain. Furthermore, we provide a comparison based on sequence conservation of all the sRNAs detected by RNA-seq in the Pseudomonas species investigated so far. Our results show that the extent of sRNA conservation across different species is very limited. In addition, when comparing the sRNAs expressed in different strains of the same species, we observe that numerous sRNAs exhibit a strain-specific expression pattern. These results support the idea that the evolution of most bacterial sRNAs is rapid, which limits the extent of both interspecies and intraspecies conservation. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. |
| author |
Tribelli, Paula María |
| author_facet |
Tribelli, Paula María |
| author_sort |
Tribelli, Paula María |
| title |
Diversity of small RNAs expressed in Pseudomonas species |
| title_short |
Diversity of small RNAs expressed in Pseudomonas species |
| title_full |
Diversity of small RNAs expressed in Pseudomonas species |
| title_fullStr |
Diversity of small RNAs expressed in Pseudomonas species |
| title_full_unstemmed |
Diversity of small RNAs expressed in Pseudomonas species |
| title_sort |
diversity of small rnas expressed in pseudomonas species |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17582229_v7_n2_p227_GomezLozano http://hdl.handle.net/20.500.12110/paper_17582229_v7_n2_p227_GomezLozano |
| work_keys_str_mv |
AT tribellipaulamaria diversityofsmallrnasexpressedinpseudomonasspecies |
| _version_ |
1768542906982858752 |