Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina)
We assessed the influence of environmental factors in shaping the free-living bacterial community structure in a set of shallow lakes characterized by contrasting stable state patterns (clear-vegetated, inorganic-turbid and phytoplankton-turbid). Six temperate shallow lakes from the Pampa Plain (Arg...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17582229_v5_n2_p310_Llames http://hdl.handle.net/20.500.12110/paper_17582229_v5_n2_p310_Llames |
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paper:paper_17582229_v5_n2_p310_Llames2023-06-08T16:29:00Z Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina) Llames, María Eugenia del Rosario Del Giorgio, Pablo A. Zagarese, Horacio Ernesto Ferraro, Marcela Andrea Izaguirre, Irina Argentina article bacterium biodiversity chemistry classification denaturing gradient gel electrophoresis genetics growth, development and aging isolation and purification lake microbiology phylogeny plankton season Argentina Bacteria Biodiversity Denaturing Gradient Gel Electrophoresis Lakes Phylogeny Plankton Seasons We assessed the influence of environmental factors in shaping the free-living bacterial community structure in a set of shallow lakes characterized by contrasting stable state patterns (clear-vegetated, inorganic-turbid and phytoplankton-turbid). Six temperate shallow lakes from the Pampa Plain (Argentina) were sampled over an annual cycle, and two fingerprinting techniques were applied: a 16S rDNA analysis was performed using denaturing gradient gel electrophoresis (DGGE) profiles, and a 16S-23S internally transcribed spacer region analysis was conducted by means of automated ribosomal intergenic spacer analysis (ARISA) profiles. Our results show that the steady state that characterized the different shallow lakes played a major role in structuring the community: the composition of free-living bacteria differed significantly between clear-vegetated, inorganic-turbid and phytoplankton-turbid shallow lakes. The state of the system was more important in determining these patterns than seasonality, geographical location or degree of hydrological connectivity. Moreover, this strong environmental control was particularly evident in the pattern observed in one of the lakes, which shifted from a clear to a turbid state over the course of the study. This lake showed a directional selection of species from a typical clear-like to a turbid-like community. The combined DGGE/ARISA approach revealed not only broad patterns among different alternative steady states, but also more subtle differences within different regimes. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd. Fil:Llames, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Giorgio, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zagarese, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferraro, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17582229_v5_n2_p310_Llames http://hdl.handle.net/20.500.12110/paper_17582229_v5_n2_p310_Llames |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Argentina article bacterium biodiversity chemistry classification denaturing gradient gel electrophoresis genetics growth, development and aging isolation and purification lake microbiology phylogeny plankton season Argentina Bacteria Biodiversity Denaturing Gradient Gel Electrophoresis Lakes Phylogeny Plankton Seasons |
| spellingShingle |
Argentina article bacterium biodiversity chemistry classification denaturing gradient gel electrophoresis genetics growth, development and aging isolation and purification lake microbiology phylogeny plankton season Argentina Bacteria Biodiversity Denaturing Gradient Gel Electrophoresis Lakes Phylogeny Plankton Seasons Llames, María Eugenia del Rosario Del Giorgio, Pablo A. Zagarese, Horacio Ernesto Ferraro, Marcela Andrea Izaguirre, Irina Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina) |
| topic_facet |
Argentina article bacterium biodiversity chemistry classification denaturing gradient gel electrophoresis genetics growth, development and aging isolation and purification lake microbiology phylogeny plankton season Argentina Bacteria Biodiversity Denaturing Gradient Gel Electrophoresis Lakes Phylogeny Plankton Seasons |
| description |
We assessed the influence of environmental factors in shaping the free-living bacterial community structure in a set of shallow lakes characterized by contrasting stable state patterns (clear-vegetated, inorganic-turbid and phytoplankton-turbid). Six temperate shallow lakes from the Pampa Plain (Argentina) were sampled over an annual cycle, and two fingerprinting techniques were applied: a 16S rDNA analysis was performed using denaturing gradient gel electrophoresis (DGGE) profiles, and a 16S-23S internally transcribed spacer region analysis was conducted by means of automated ribosomal intergenic spacer analysis (ARISA) profiles. Our results show that the steady state that characterized the different shallow lakes played a major role in structuring the community: the composition of free-living bacteria differed significantly between clear-vegetated, inorganic-turbid and phytoplankton-turbid shallow lakes. The state of the system was more important in determining these patterns than seasonality, geographical location or degree of hydrological connectivity. Moreover, this strong environmental control was particularly evident in the pattern observed in one of the lakes, which shifted from a clear to a turbid state over the course of the study. This lake showed a directional selection of species from a typical clear-like to a turbid-like community. The combined DGGE/ARISA approach revealed not only broad patterns among different alternative steady states, but also more subtle differences within different regimes. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd. |
| author |
Llames, María Eugenia del Rosario Del Giorgio, Pablo A. Zagarese, Horacio Ernesto Ferraro, Marcela Andrea Izaguirre, Irina |
| author_facet |
Llames, María Eugenia del Rosario Del Giorgio, Pablo A. Zagarese, Horacio Ernesto Ferraro, Marcela Andrea Izaguirre, Irina |
| author_sort |
Llames, María Eugenia del Rosario |
| title |
Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina) |
| title_short |
Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina) |
| title_full |
Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina) |
| title_fullStr |
Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina) |
| title_full_unstemmed |
Alternative states drive the patterns in the bacterioplankton composition in shallow Pampean lakes (Argentina) |
| title_sort |
alternative states drive the patterns in the bacterioplankton composition in shallow pampean lakes (argentina) |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17582229_v5_n2_p310_Llames http://hdl.handle.net/20.500.12110/paper_17582229_v5_n2_p310_Llames |
| work_keys_str_mv |
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| _version_ |
1768543007273910272 |