Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study

Background: In assisted reproduction cycles, gonadotropins are administered to obtain a greater number of oocytes. A majority of patients do not have an adverse response; however, approximately 3-6% develop ovarian hyperstimulation syndrome (OHSS). Metformin reduces the risk of OHSS but little is kn...

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Autores principales:	Elia, Evelin Mariel, Paz, Dante Agustin, Pustovrh, Maria Carolina
Publicado:	2013
Materias:	cyclooxygenase 2 estradiol Evans blue metformin nitric oxide synthase progesterone vasculotropin animal experiment animal model article blood vessel permeability body weight controlled study enzyme linked immunosorbent assay female immunohistochemistry nonhuman ovary follicle ovary hyperstimulation pathophysiology priority journal protein expression rat Western blotting
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17572215_v6_n1_p_Elia http://hdl.handle.net/20.500.12110/paper_17572215_v6_n1_p_Elia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_17572215_v6_n1_p_Elia
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spelling	paper:paper_17572215_v6_n1_p_Elia2023-06-08T16:28:56Z Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study Elia, Evelin Mariel Paz, Dante Agustin Pustovrh, Maria Carolina cyclooxygenase 2 estradiol Evans blue metformin nitric oxide synthase progesterone vasculotropin animal experiment animal model article blood vessel permeability body weight controlled study enzyme linked immunosorbent assay female immunohistochemistry nonhuman ovary follicle ovary hyperstimulation pathophysiology priority journal protein expression rat Western blotting Background: In assisted reproduction cycles, gonadotropins are administered to obtain a greater number of oocytes. A majority of patients do not have an adverse response; however, approximately 3-6% develop ovarian hyperstimulation syndrome (OHSS). Metformin reduces the risk of OHSS but little is known about the possible effects and mechanisms of action involved. Objective. To evaluate whether metformin attenuates some of the ovarian adverse effects caused by OHSS and to study the mechanisms involved. Material and methods. A rat OHSS model was used to investigate the effects of metformin administration. Ovarian histology and follicle counting were performed in ovarian sections stained with Masson trichrome. Vascular permeability was measured by the release of intravenously injected Evans Blue dye (EB). VEGF levels were measured by commercially immunosorbent assay kit. COX-2 protein expression was evaluated by western blot and NOS levels were analyses by immunohistochemistry. Results: Animals of the OHSS group showed similar physiopathology characteristics to the human syndrome: increased body weight, elevated progesterone and estradiol levels (P<0.001), increased number of corpora lutea (P<0.001), higher ovarian VEGF levels and vascular permeability (P<0.001 and P<0.01); and treatment with metformin prevented this effect (OHSS+M group; P<0.05). The vasoactive factors: COX-2 and NOS were increased in the ovaries of the OHSS group (P<0.05 and P<0.01) and metformin normalized their expression (P<0.05); suggesting that metformin has a role preventing the increased in vascular permeability caused by the syndrome. Conclusion: Metformin has a beneficial effect preventing OHSS by reducing the increase in: body weight, circulating progesterone and estradiol and vascular permeability. These effects of metformin are mediated by inhibiting the increased of the vasoactive molecules: VEGF, COX-2 and partially NOS. Molecules that are increased in OHSS and are responsible for a variety of the symptoms related to OHSS. © 2013 Elia et al.; licensee BioMed Central Ltd. Fil:Elia, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pustovrh, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17572215_v6_n1_p_Elia http://hdl.handle.net/20.500.12110/paper_17572215_v6_n1_p_Elia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyclooxygenase 2 estradiol Evans blue metformin nitric oxide synthase progesterone vasculotropin animal experiment animal model article blood vessel permeability body weight controlled study enzyme linked immunosorbent assay female immunohistochemistry nonhuman ovary follicle ovary hyperstimulation pathophysiology priority journal protein expression rat Western blotting
spellingShingle	cyclooxygenase 2 estradiol Evans blue metformin nitric oxide synthase progesterone vasculotropin animal experiment animal model article blood vessel permeability body weight controlled study enzyme linked immunosorbent assay female immunohistochemistry nonhuman ovary follicle ovary hyperstimulation pathophysiology priority journal protein expression rat Western blotting Elia, Evelin Mariel Paz, Dante Agustin Pustovrh, Maria Carolina Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study
topic_facet	cyclooxygenase 2 estradiol Evans blue metformin nitric oxide synthase progesterone vasculotropin animal experiment animal model article blood vessel permeability body weight controlled study enzyme linked immunosorbent assay female immunohistochemistry nonhuman ovary follicle ovary hyperstimulation pathophysiology priority journal protein expression rat Western blotting
description	Background: In assisted reproduction cycles, gonadotropins are administered to obtain a greater number of oocytes. A majority of patients do not have an adverse response; however, approximately 3-6% develop ovarian hyperstimulation syndrome (OHSS). Metformin reduces the risk of OHSS but little is known about the possible effects and mechanisms of action involved. Objective. To evaluate whether metformin attenuates some of the ovarian adverse effects caused by OHSS and to study the mechanisms involved. Material and methods. A rat OHSS model was used to investigate the effects of metformin administration. Ovarian histology and follicle counting were performed in ovarian sections stained with Masson trichrome. Vascular permeability was measured by the release of intravenously injected Evans Blue dye (EB). VEGF levels were measured by commercially immunosorbent assay kit. COX-2 protein expression was evaluated by western blot and NOS levels were analyses by immunohistochemistry. Results: Animals of the OHSS group showed similar physiopathology characteristics to the human syndrome: increased body weight, elevated progesterone and estradiol levels (P<0.001), increased number of corpora lutea (P<0.001), higher ovarian VEGF levels and vascular permeability (P<0.001 and P<0.01); and treatment with metformin prevented this effect (OHSS+M group; P<0.05). The vasoactive factors: COX-2 and NOS were increased in the ovaries of the OHSS group (P<0.05 and P<0.01) and metformin normalized their expression (P<0.05); suggesting that metformin has a role preventing the increased in vascular permeability caused by the syndrome. Conclusion: Metformin has a beneficial effect preventing OHSS by reducing the increase in: body weight, circulating progesterone and estradiol and vascular permeability. These effects of metformin are mediated by inhibiting the increased of the vasoactive molecules: VEGF, COX-2 and partially NOS. Molecules that are increased in OHSS and are responsible for a variety of the symptoms related to OHSS. © 2013 Elia et al.; licensee BioMed Central Ltd.
author	Elia, Evelin Mariel Paz, Dante Agustin Pustovrh, Maria Carolina
author_facet	Elia, Evelin Mariel Paz, Dante Agustin Pustovrh, Maria Carolina
author_sort	Elia, Evelin Mariel
title	Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study
title_short	Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study
title_full	Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study
title_fullStr	Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study
title_full_unstemmed	Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study
title_sort	metformin decreases the incidence of ovarian hyperstimulation syndrome: an experimental study
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17572215_v6_n1_p_Elia http://hdl.handle.net/20.500.12110/paper_17572215_v6_n1_p_Elia
work_keys_str_mv	AT eliaevelinmariel metformindecreasestheincidenceofovarianhyperstimulationsyndromeanexperimentalstudy AT pazdanteagustin metformindecreasestheincidenceofovarianhyperstimulationsyndromeanexperimentalstudy AT pustovrhmariacarolina metformindecreasestheincidenceofovarianhyperstimulationsyndromeanexperimentalstudy
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Metformin decreases the incidence of ovarian hyperstimulation syndrome: An experimental study

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