Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines
Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hip...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17563305_v9_n1_p_Guillemi http://hdl.handle.net/20.500.12110/paper_17563305_v9_n1_p_Guillemi |
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paper:paper_17563305_v9_n1_p_Guillemi2023-06-08T16:28:54Z Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines Anaplasma marginale Giant anteater Taruca Wild species algorithm anaplasmosis anteater Argentina Article bacterial gene bacterium identification bovine gene amplification gene sequence genotype Hippocamelus antisense msp5 gene Myrmecophaga tridactyla nonhuman phylogeny South America tandem repeat wild species Anaplasma marginale anaplasmosis animal Artiodactyla blood bovine case report cattle disease classification DNA sequence female genetics genotype host range isolation and purification microbiology multilocus sequence typing phylogeny veterinary Xenarthra bacterial DNA major surface protein 1a, Anaplasma major surface protein 5, Anaplasma outer membrane protein Anaplasma marginale Anaplasmosis Animals Artiodactyla Bacterial Outer Membrane Proteins Cattle Cattle Diseases DNA, Bacterial Female Genotype Host Specificity Multilocus Sequence Typing Phylogeny Sequence Analysis, DNA Tandem Repeat Sequences Xenarthra Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group. Conclusions: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology. © 2016 Guillemi et al. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17563305_v9_n1_p_Guillemi http://hdl.handle.net/20.500.12110/paper_17563305_v9_n1_p_Guillemi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anaplasma marginale Giant anteater Taruca Wild species algorithm anaplasmosis anteater Argentina Article bacterial gene bacterium identification bovine gene amplification gene sequence genotype Hippocamelus antisense msp5 gene Myrmecophaga tridactyla nonhuman phylogeny South America tandem repeat wild species Anaplasma marginale anaplasmosis animal Artiodactyla blood bovine case report cattle disease classification DNA sequence female genetics genotype host range isolation and purification microbiology multilocus sequence typing phylogeny veterinary Xenarthra bacterial DNA major surface protein 1a, Anaplasma major surface protein 5, Anaplasma outer membrane protein Anaplasma marginale Anaplasmosis Animals Artiodactyla Bacterial Outer Membrane Proteins Cattle Cattle Diseases DNA, Bacterial Female Genotype Host Specificity Multilocus Sequence Typing Phylogeny Sequence Analysis, DNA Tandem Repeat Sequences Xenarthra |
| spellingShingle |
Anaplasma marginale Giant anteater Taruca Wild species algorithm anaplasmosis anteater Argentina Article bacterial gene bacterium identification bovine gene amplification gene sequence genotype Hippocamelus antisense msp5 gene Myrmecophaga tridactyla nonhuman phylogeny South America tandem repeat wild species Anaplasma marginale anaplasmosis animal Artiodactyla blood bovine case report cattle disease classification DNA sequence female genetics genotype host range isolation and purification microbiology multilocus sequence typing phylogeny veterinary Xenarthra bacterial DNA major surface protein 1a, Anaplasma major surface protein 5, Anaplasma outer membrane protein Anaplasma marginale Anaplasmosis Animals Artiodactyla Bacterial Outer Membrane Proteins Cattle Cattle Diseases DNA, Bacterial Female Genotype Host Specificity Multilocus Sequence Typing Phylogeny Sequence Analysis, DNA Tandem Repeat Sequences Xenarthra Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| topic_facet |
Anaplasma marginale Giant anteater Taruca Wild species algorithm anaplasmosis anteater Argentina Article bacterial gene bacterium identification bovine gene amplification gene sequence genotype Hippocamelus antisense msp5 gene Myrmecophaga tridactyla nonhuman phylogeny South America tandem repeat wild species Anaplasma marginale anaplasmosis animal Artiodactyla blood bovine case report cattle disease classification DNA sequence female genetics genotype host range isolation and purification microbiology multilocus sequence typing phylogeny veterinary Xenarthra bacterial DNA major surface protein 1a, Anaplasma major surface protein 5, Anaplasma outer membrane protein Anaplasma marginale Anaplasmosis Animals Artiodactyla Bacterial Outer Membrane Proteins Cattle Cattle Diseases DNA, Bacterial Female Genotype Host Specificity Multilocus Sequence Typing Phylogeny Sequence Analysis, DNA Tandem Repeat Sequences Xenarthra |
| description |
Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group. Conclusions: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology. © 2016 Guillemi et al. |
| title |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_short |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_full |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_fullStr |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_full_unstemmed |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_sort |
molecular identification of anaplasma marginale in two autochthonous south american wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17563305_v9_n1_p_Guillemi http://hdl.handle.net/20.500.12110/paper_17563305_v9_n1_p_Guillemi |
| _version_ |
1768546742895116288 |