Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period
Background: Maternal antigen-presenting cells attracted to the pregnant uterus interact with trophoblast cells and modulate their functional profile to favour immunosuppressant responses. Non-neuronal cholinergic system is expressed in human cytotrophoblast cells and in immune cells with homeostatic...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17481708_v214_n2_p237_Paparini http://hdl.handle.net/20.500.12110/paper_17481708_v214_n2_p237_Paparini |
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paper:paper_17481708_v214_n2_p237_Paparini2023-06-08T16:28:37Z Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period Grasso, Esteban Nicolás Pérez Leirós, Claudia Ramhorst, Rosanna Elizabeth Salamone, Gabriela V. Dendritic cells Monocytes Non-neuronal acetylcholine Trophoblast cells acetylcholine atropine CD83 antigen CD86 antigen HLA DR antigen interleukin 10 interleukin 6 macrophage inflammatory protein 1alpha monocyte chemotactic protein 1 muscarinic receptor neostigmine RANTES tumor necrosis factor alpha acetylcholine antigen presenting cell Article cell interaction coculture controlled study cytokine production cytotrophoblast dendritic cell female human human cell immunomodulation in vitro study inflammation leukocyte activation leukocyte migration monocyte nidation placenta priority journal protein expression protein synthesis swan 71 cell line trophoblast cell separation cytology immunology inflammation metabolism nidation pregnancy procedures trophoblast Acetylcholine Cell Separation Coculture Techniques Embryo Implantation Female Humans Inflammation Placenta Pregnancy Trophoblasts Background: Maternal antigen-presenting cells attracted to the pregnant uterus interact with trophoblast cells and modulate their functional profile to favour immunosuppressant responses. Non-neuronal cholinergic system is expressed in human cytotrophoblast cells and in immune cells with homeostatic regulatory functions. Aim: The aim of this work was to evaluate whether non-neuronal acetylcholine conditions maternal monocyte and DC migration and activation profiles. Methods: We used an in vitro model resembling maternal-placental interface represented by the co-culture of human trophoblast cells (Swan-71 cell line) and monocytes or DC. Results: When cytotrophoblast cells were treated with neostigmine (Neo) to concentrate endogenous acetylcholine levels, monocyte migration was increased. In parallel, high levels of IL-10 and decreased levels of TNF-α were observed upon interaction of maternal monocytes with trophoblast cells. This effect was synergized by Neo and was prevented by atropine, a muscarinic acetylcholine receptor antagonist. Similarly, trophoblast cells increased the migration of DC independently of Neo treatment; however, enhanced IL-10 and MCP-1 synthesis in trophoblast-DC co-cultures with no changes in TNF-α and IL-6 was observed. In fact, there were no changes in HLA-DR, CD86 or CD83 expression. Finally, trophoblast cells treated with Neo increased the expression of two antigen-presenting cells attracting chemokines, MCP-1, MIP-1α and RANTES through muscarinic receptors, and it was prevented by atropine. Conclusions: Our present results support a novel role of acetylcholine synthesized by trophoblast cells to modulate antigen-presenting cell migration and activation favouring an immunosuppressant profile that contributes to immune homeostasis maintenance at the maternal-foetal interface. © 2015 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd. Fil:Grasso, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez Leirós, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramhorst, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salamone, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17481708_v214_n2_p237_Paparini http://hdl.handle.net/20.500.12110/paper_17481708_v214_n2_p237_Paparini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dendritic cells Monocytes Non-neuronal acetylcholine Trophoblast cells acetylcholine atropine CD83 antigen CD86 antigen HLA DR antigen interleukin 10 interleukin 6 macrophage inflammatory protein 1alpha monocyte chemotactic protein 1 muscarinic receptor neostigmine RANTES tumor necrosis factor alpha acetylcholine antigen presenting cell Article cell interaction coculture controlled study cytokine production cytotrophoblast dendritic cell female human human cell immunomodulation in vitro study inflammation leukocyte activation leukocyte migration monocyte nidation placenta priority journal protein expression protein synthesis swan 71 cell line trophoblast cell separation cytology immunology inflammation metabolism nidation pregnancy procedures trophoblast Acetylcholine Cell Separation Coculture Techniques Embryo Implantation Female Humans Inflammation Placenta Pregnancy Trophoblasts |
| spellingShingle |
Dendritic cells Monocytes Non-neuronal acetylcholine Trophoblast cells acetylcholine atropine CD83 antigen CD86 antigen HLA DR antigen interleukin 10 interleukin 6 macrophage inflammatory protein 1alpha monocyte chemotactic protein 1 muscarinic receptor neostigmine RANTES tumor necrosis factor alpha acetylcholine antigen presenting cell Article cell interaction coculture controlled study cytokine production cytotrophoblast dendritic cell female human human cell immunomodulation in vitro study inflammation leukocyte activation leukocyte migration monocyte nidation placenta priority journal protein expression protein synthesis swan 71 cell line trophoblast cell separation cytology immunology inflammation metabolism nidation pregnancy procedures trophoblast Acetylcholine Cell Separation Coculture Techniques Embryo Implantation Female Humans Inflammation Placenta Pregnancy Trophoblasts Grasso, Esteban Nicolás Pérez Leirós, Claudia Ramhorst, Rosanna Elizabeth Salamone, Gabriela V. Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period |
| topic_facet |
Dendritic cells Monocytes Non-neuronal acetylcholine Trophoblast cells acetylcholine atropine CD83 antigen CD86 antigen HLA DR antigen interleukin 10 interleukin 6 macrophage inflammatory protein 1alpha monocyte chemotactic protein 1 muscarinic receptor neostigmine RANTES tumor necrosis factor alpha acetylcholine antigen presenting cell Article cell interaction coculture controlled study cytokine production cytotrophoblast dendritic cell female human human cell immunomodulation in vitro study inflammation leukocyte activation leukocyte migration monocyte nidation placenta priority journal protein expression protein synthesis swan 71 cell line trophoblast cell separation cytology immunology inflammation metabolism nidation pregnancy procedures trophoblast Acetylcholine Cell Separation Coculture Techniques Embryo Implantation Female Humans Inflammation Placenta Pregnancy Trophoblasts |
| description |
Background: Maternal antigen-presenting cells attracted to the pregnant uterus interact with trophoblast cells and modulate their functional profile to favour immunosuppressant responses. Non-neuronal cholinergic system is expressed in human cytotrophoblast cells and in immune cells with homeostatic regulatory functions. Aim: The aim of this work was to evaluate whether non-neuronal acetylcholine conditions maternal monocyte and DC migration and activation profiles. Methods: We used an in vitro model resembling maternal-placental interface represented by the co-culture of human trophoblast cells (Swan-71 cell line) and monocytes or DC. Results: When cytotrophoblast cells were treated with neostigmine (Neo) to concentrate endogenous acetylcholine levels, monocyte migration was increased. In parallel, high levels of IL-10 and decreased levels of TNF-α were observed upon interaction of maternal monocytes with trophoblast cells. This effect was synergized by Neo and was prevented by atropine, a muscarinic acetylcholine receptor antagonist. Similarly, trophoblast cells increased the migration of DC independently of Neo treatment; however, enhanced IL-10 and MCP-1 synthesis in trophoblast-DC co-cultures with no changes in TNF-α and IL-6 was observed. In fact, there were no changes in HLA-DR, CD86 or CD83 expression. Finally, trophoblast cells treated with Neo increased the expression of two antigen-presenting cells attracting chemokines, MCP-1, MIP-1α and RANTES through muscarinic receptors, and it was prevented by atropine. Conclusions: Our present results support a novel role of acetylcholine synthesized by trophoblast cells to modulate antigen-presenting cell migration and activation favouring an immunosuppressant profile that contributes to immune homeostasis maintenance at the maternal-foetal interface. © 2015 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd. |
| author |
Grasso, Esteban Nicolás Pérez Leirós, Claudia Ramhorst, Rosanna Elizabeth Salamone, Gabriela V. |
| author_facet |
Grasso, Esteban Nicolás Pérez Leirós, Claudia Ramhorst, Rosanna Elizabeth Salamone, Gabriela V. |
| author_sort |
Grasso, Esteban Nicolás |
| title |
Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period |
| title_short |
Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period |
| title_full |
Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period |
| title_fullStr |
Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period |
| title_full_unstemmed |
Acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period |
| title_sort |
acetylcholine contributes to control the physiological inflammatory response during the peri-implantation period |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17481708_v214_n2_p237_Paparini http://hdl.handle.net/20.500.12110/paper_17481708_v214_n2_p237_Paparini |
| work_keys_str_mv |
AT grassoestebannicolas acetylcholinecontributestocontrolthephysiologicalinflammatoryresponseduringtheperiimplantationperiod AT perezleirosclaudia acetylcholinecontributestocontrolthephysiologicalinflammatoryresponseduringtheperiimplantationperiod AT ramhorstrosannaelizabeth acetylcholinecontributestocontrolthephysiologicalinflammatoryresponseduringtheperiimplantationperiod AT salamonegabrielav acetylcholinecontributestocontrolthephysiologicalinflammatoryresponseduringtheperiimplantationperiod |
| _version_ |
1768546088658141184 |