The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics
The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable ident...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1742464X_v276_n8_p2266_Giordano http://hdl.handle.net/20.500.12110/paper_1742464X_v276_n8_p2266_Giordano |
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paper:paper_1742464X_v276_n8_p2266_Giordano2023-06-08T16:27:02Z The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics Antarctica Computer simulation Hemoglobin Ligand-binding properties Oxygen affinity hemoglobin hemoglobin beta chain protein Antarctica article Bohr effect Cottoperca gobio fish hemoglobin analysis kinetics latitude migration modulation molecular dynamics nonhuman oxygen affinity priority journal simulation teleost Amino Acid Sequence Animals Binding Sites Computer Simulation Fishes Hemoglobins Hydrogen-Ion Concentration Kinetics Molecular Sequence Data Oxygen Phylogeny Species Specificity Structure-Activity Relationship Thermodynamics Acanthomorpha Bovichthyidae Cottoperca gobio Notothenioidei Perciformes Teleostei The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history. Non-Antarctic notothenioids belonging to the most basal families are a crucial source for understanding the evolution of hemoglobin in high-Antarctic cold-adapted fish. This paper focuses on the structure, function and evolution of the oxygen-transport system of Cottoperca gobio, a sub-Antarctic notothenioid fish of the family Bovichtidae, probably derived from ancestral species that evolved in the Antarctic region and later migrated to lower latitudes. Unlike most high-Antarctic notothenioids, but similar to many other acanthomorph teleosts, C. gobio has two major hemoglobins having the β chain in common. The oxygen-binding equilibria and kinetics of the two hemoglobins have been measured. Hb1 and Hb2 show strong modulation of oxygen-binding equilibria and kinetics by heterotropic effectors, with marked Bohr and Root effects. In Hb1 and Hb2, oxygen affinity and subunit cooperativity are slightly higher than in most high-Antarctic notothenioid hemoglobins. Hb1 and Hb2 show similar rebinding rates, but also show significant dynamic differences that are likely to have functional consequences. Molecular dynamic simulations of C. gobio Hb1 were performed on the dimeric protein in order to obtain a better understanding of the molecular basis of structure/function relationships. © 2009 FEBS. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1742464X_v276_n8_p2266_Giordano http://hdl.handle.net/20.500.12110/paper_1742464X_v276_n8_p2266_Giordano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antarctica Computer simulation Hemoglobin Ligand-binding properties Oxygen affinity hemoglobin hemoglobin beta chain protein Antarctica article Bohr effect Cottoperca gobio fish hemoglobin analysis kinetics latitude migration modulation molecular dynamics nonhuman oxygen affinity priority journal simulation teleost Amino Acid Sequence Animals Binding Sites Computer Simulation Fishes Hemoglobins Hydrogen-Ion Concentration Kinetics Molecular Sequence Data Oxygen Phylogeny Species Specificity Structure-Activity Relationship Thermodynamics Acanthomorpha Bovichthyidae Cottoperca gobio Notothenioidei Perciformes Teleostei |
| spellingShingle |
Antarctica Computer simulation Hemoglobin Ligand-binding properties Oxygen affinity hemoglobin hemoglobin beta chain protein Antarctica article Bohr effect Cottoperca gobio fish hemoglobin analysis kinetics latitude migration modulation molecular dynamics nonhuman oxygen affinity priority journal simulation teleost Amino Acid Sequence Animals Binding Sites Computer Simulation Fishes Hemoglobins Hydrogen-Ion Concentration Kinetics Molecular Sequence Data Oxygen Phylogeny Species Specificity Structure-Activity Relationship Thermodynamics Acanthomorpha Bovichthyidae Cottoperca gobio Notothenioidei Perciformes Teleostei The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
| topic_facet |
Antarctica Computer simulation Hemoglobin Ligand-binding properties Oxygen affinity hemoglobin hemoglobin beta chain protein Antarctica article Bohr effect Cottoperca gobio fish hemoglobin analysis kinetics latitude migration modulation molecular dynamics nonhuman oxygen affinity priority journal simulation teleost Amino Acid Sequence Animals Binding Sites Computer Simulation Fishes Hemoglobins Hydrogen-Ion Concentration Kinetics Molecular Sequence Data Oxygen Phylogeny Species Specificity Structure-Activity Relationship Thermodynamics Acanthomorpha Bovichthyidae Cottoperca gobio Notothenioidei Perciformes Teleostei |
| description |
The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history. Non-Antarctic notothenioids belonging to the most basal families are a crucial source for understanding the evolution of hemoglobin in high-Antarctic cold-adapted fish. This paper focuses on the structure, function and evolution of the oxygen-transport system of Cottoperca gobio, a sub-Antarctic notothenioid fish of the family Bovichtidae, probably derived from ancestral species that evolved in the Antarctic region and later migrated to lower latitudes. Unlike most high-Antarctic notothenioids, but similar to many other acanthomorph teleosts, C. gobio has two major hemoglobins having the β chain in common. The oxygen-binding equilibria and kinetics of the two hemoglobins have been measured. Hb1 and Hb2 show strong modulation of oxygen-binding equilibria and kinetics by heterotropic effectors, with marked Bohr and Root effects. In Hb1 and Hb2, oxygen affinity and subunit cooperativity are slightly higher than in most high-Antarctic notothenioid hemoglobins. Hb1 and Hb2 show similar rebinding rates, but also show significant dynamic differences that are likely to have functional consequences. Molecular dynamic simulations of C. gobio Hb1 were performed on the dimeric protein in order to obtain a better understanding of the molecular basis of structure/function relationships. © 2009 FEBS. |
| title |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
| title_short |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
| title_full |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
| title_fullStr |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
| title_full_unstemmed |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
| title_sort |
hemoglobins of the sub-antarctic fish cottoperca gobio, a phyletically basal species - oxygen-binding equilibria, kinetics and molecular dynamics |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1742464X_v276_n8_p2266_Giordano http://hdl.handle.net/20.500.12110/paper_1742464X_v276_n8_p2266_Giordano |
| _version_ |
1768545162980491264 |