Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos

Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolu...

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Autor principal: Levi, Valeria
Publicado: 2016
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17417007_v14_n1_p_Zhao
http://hdl.handle.net/20.500.12110/paper_17417007_v14_n1_p_Zhao
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spelling paper:paper_17417007_v14_n1_p_Zhao2023-06-08T16:27:00Z Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos Levi, Valeria animal cell nucleus embryology fluorescence microscopy gene expression regulation genetic epigenesis genetics mammal single cell analysis spatiotemporal analysis transcription initiation Animals Cell Nucleus Epigenesis, Genetic Gene Expression Regulation Mammals Microscopy, Fluorescence Single-Cell Analysis Spatio-Temporal Analysis Transcriptional Activation Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos. © 2016 Plachta et al. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17417007_v14_n1_p_Zhao http://hdl.handle.net/20.500.12110/paper_17417007_v14_n1_p_Zhao
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic animal
cell nucleus
embryology
fluorescence microscopy
gene expression regulation
genetic epigenesis
genetics
mammal
single cell analysis
spatiotemporal analysis
transcription initiation
Animals
Cell Nucleus
Epigenesis, Genetic
Gene Expression Regulation
Mammals
Microscopy, Fluorescence
Single-Cell Analysis
Spatio-Temporal Analysis
Transcriptional Activation
spellingShingle animal
cell nucleus
embryology
fluorescence microscopy
gene expression regulation
genetic epigenesis
genetics
mammal
single cell analysis
spatiotemporal analysis
transcription initiation
Animals
Cell Nucleus
Epigenesis, Genetic
Gene Expression Regulation
Mammals
Microscopy, Fluorescence
Single-Cell Analysis
Spatio-Temporal Analysis
Transcriptional Activation
Levi, Valeria
Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
topic_facet animal
cell nucleus
embryology
fluorescence microscopy
gene expression regulation
genetic epigenesis
genetics
mammal
single cell analysis
spatiotemporal analysis
transcription initiation
Animals
Cell Nucleus
Epigenesis, Genetic
Gene Expression Regulation
Mammals
Microscopy, Fluorescence
Single-Cell Analysis
Spatio-Temporal Analysis
Transcriptional Activation
description Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos. © 2016 Plachta et al.
author Levi, Valeria
author_facet Levi, Valeria
author_sort Levi, Valeria
title Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
title_short Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
title_full Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
title_fullStr Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
title_full_unstemmed Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
title_sort quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17417007_v14_n1_p_Zhao
http://hdl.handle.net/20.500.12110/paper_17417007_v14_n1_p_Zhao
work_keys_str_mv AT levivaleria quantitativeimagingofmammaliantranscriptionaldynamicsfromsinglecellstowholeembryos
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