Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination

The megagametophyte of mature seeds of Araucaria angustifolia consists of cells with thin walls, one or more nuclei, a central vacuole storing proteins, and a cytoplasm rich in amyloplasts, mitochondria and lipid bodies. In this study, we describe the process of mobilization of reserves and analyzed...

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Detalles Bibliográficos
Publicado:	2018
Materias:	Araucaria angustifolia Cys-EP Germination Megagametophyte Nucleases PCD Starch
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1664462X_v9_n_p_Moyano http://hdl.handle.net/20.500.12110/paper_1664462X_v9_n_p_Moyano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1664462X_v9_n_p_Moyano
record_format	dspace
spelling	paper:paper_1664462X_v9_n_p_Moyano2023-06-08T16:26:09Z Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination Araucaria angustifolia Cys-EP Germination Megagametophyte Nucleases PCD Starch The megagametophyte of mature seeds of Araucaria angustifolia consists of cells with thin walls, one or more nuclei, a central vacuole storing proteins, and a cytoplasm rich in amyloplasts, mitochondria and lipid bodies. In this study, we describe the process of mobilization of reserves and analyzed the dismantling of the tissue during germination, using a range of well-established markers of programmed cell death (PCD), including: morphological changes in nuclei and amyloplasts, DNA degradation, and changes in nuclease profiles. TUNEL reaction and DNA electrophoresis demonstrate that DNA fragmentation in nuclei occurs at early stages of germination, which correlates with induction of specific nucleases. The results of the present study add knowledge on the dismantling of the megagametophyte of genus Araucaria, a storage tissue that stores starch as the main reserve substance, as well as on the PCD pathway, by revealing new insights into the role of nucleases and the expression patterns of putative nuclease genes during germination. © 2018 Moyano, Correa, Favre, Rodríguez, Maldonado and López-Fernández. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1664462X_v9_n_p_Moyano http://hdl.handle.net/20.500.12110/paper_1664462X_v9_n_p_Moyano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Araucaria angustifolia Cys-EP Germination Megagametophyte Nucleases PCD Starch
spellingShingle	Araucaria angustifolia Cys-EP Germination Megagametophyte Nucleases PCD Starch Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination
topic_facet	Araucaria angustifolia Cys-EP Germination Megagametophyte Nucleases PCD Starch
description	The megagametophyte of mature seeds of Araucaria angustifolia consists of cells with thin walls, one or more nuclei, a central vacuole storing proteins, and a cytoplasm rich in amyloplasts, mitochondria and lipid bodies. In this study, we describe the process of mobilization of reserves and analyzed the dismantling of the tissue during germination, using a range of well-established markers of programmed cell death (PCD), including: morphological changes in nuclei and amyloplasts, DNA degradation, and changes in nuclease profiles. TUNEL reaction and DNA electrophoresis demonstrate that DNA fragmentation in nuclei occurs at early stages of germination, which correlates with induction of specific nucleases. The results of the present study add knowledge on the dismantling of the megagametophyte of genus Araucaria, a storage tissue that stores starch as the main reserve substance, as well as on the PCD pathway, by revealing new insights into the role of nucleases and the expression patterns of putative nuclease genes during germination. © 2018 Moyano, Correa, Favre, Rodríguez, Maldonado and López-Fernández.
title	Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination
title_short	Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination
title_full	Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination
title_fullStr	Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination
title_full_unstemmed	Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination
title_sort	activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1664462X_v9_n_p_Moyano http://hdl.handle.net/20.500.12110/paper_1664462X_v9_n_p_Moyano
_version_	1768544522530193408

Activation of nucleases, pcd, and mobilization of reserves in the araucaria angustifolia megagametophyte during germination

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