Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process
Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains un...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16625102_v10_nJUL_p_MoyaDiaz http://hdl.handle.net/20.500.12110/paper_16625102_v10_nJUL_p_MoyaDiaz |
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paper:paper_16625102_v10_nJUL_p_MoyaDiaz2023-06-08T16:25:47Z Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process Belingheri, Ana Veronica Marengo, Fernando Diego Ca2+ current Dynamin Endocytosis Immediately releasable pool Membrane capacitance Secretion amphiphysin calcium ion dynamin hybrid protein voltage gated calcium channel Wnt2 protein action potential animal cell animal experiment animal model animal tissue Article blood vessel capacitance calcium current chromaffin cell controlled study depolarization electrostimulation endocytosis exocytosis female firing rate low frequency noise male mouse nonhuman Purkinje fiber signal processing transport vesicle whole cell patch clamp Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 ± 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (τ = 0.53 ± 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies. © 2016 Moya-Díaz, Álvarez, Montenegro, Bayonés, Belingheri, González- Jamett, Cárdenas and Marengo. Fil:Belingheri, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marengo, F.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16625102_v10_nJUL_p_MoyaDiaz http://hdl.handle.net/20.500.12110/paper_16625102_v10_nJUL_p_MoyaDiaz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ca2+ current Dynamin Endocytosis Immediately releasable pool Membrane capacitance Secretion amphiphysin calcium ion dynamin hybrid protein voltage gated calcium channel Wnt2 protein action potential animal cell animal experiment animal model animal tissue Article blood vessel capacitance calcium current chromaffin cell controlled study depolarization electrostimulation endocytosis exocytosis female firing rate low frequency noise male mouse nonhuman Purkinje fiber signal processing transport vesicle whole cell patch clamp |
| spellingShingle |
Ca2+ current Dynamin Endocytosis Immediately releasable pool Membrane capacitance Secretion amphiphysin calcium ion dynamin hybrid protein voltage gated calcium channel Wnt2 protein action potential animal cell animal experiment animal model animal tissue Article blood vessel capacitance calcium current chromaffin cell controlled study depolarization electrostimulation endocytosis exocytosis female firing rate low frequency noise male mouse nonhuman Purkinje fiber signal processing transport vesicle whole cell patch clamp Belingheri, Ana Veronica Marengo, Fernando Diego Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
| topic_facet |
Ca2+ current Dynamin Endocytosis Immediately releasable pool Membrane capacitance Secretion amphiphysin calcium ion dynamin hybrid protein voltage gated calcium channel Wnt2 protein action potential animal cell animal experiment animal model animal tissue Article blood vessel capacitance calcium current chromaffin cell controlled study depolarization electrostimulation endocytosis exocytosis female firing rate low frequency noise male mouse nonhuman Purkinje fiber signal processing transport vesicle whole cell patch clamp |
| description |
Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 ± 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (τ = 0.53 ± 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies. © 2016 Moya-Díaz, Álvarez, Montenegro, Bayonés, Belingheri, González- Jamett, Cárdenas and Marengo. |
| author |
Belingheri, Ana Veronica Marengo, Fernando Diego |
| author_facet |
Belingheri, Ana Veronica Marengo, Fernando Diego |
| author_sort |
Belingheri, Ana Veronica |
| title |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
| title_short |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
| title_full |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
| title_fullStr |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
| title_full_unstemmed |
Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
| title_sort |
sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16625102_v10_nJUL_p_MoyaDiaz http://hdl.handle.net/20.500.12110/paper_16625102_v10_nJUL_p_MoyaDiaz |
| work_keys_str_mv |
AT belingherianaveronica sustainedexocytosisafteractionpotentiallikestimulationatlowfrequenciesinmousechromaffincellsdependsonadynamindependentfastendocytoticprocess AT marengofernandodiego sustainedexocytosisafteractionpotentiallikestimulationatlowfrequenciesinmousechromaffincellsdependsonadynamindependentfastendocytoticprocess |
| _version_ |
1768544794944995328 |