Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata

We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC tech...

Descripción completa

Guardado en:

Detalles Bibliográficos
Publicado:	2018
Materias:	Axenic culture Elsinochrome A Graphis elongata Lichen Perylenequinones
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v17_n4_p479_Fazio http://hdl.handle.net/20.500.12110/paper_1617416X_v17_n4_p479_Fazio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1617416X_v17_n4_p479_Fazio
record_format	dspace
spelling	paper:paper_1617416X_v17_n4_p479_Fazio2023-06-08T16:25:30Z Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata Axenic culture Elsinochrome A Graphis elongata Lichen Perylenequinones We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC techniques) and is otherwise only known from one unrelated lichen and a few genera of non-lichenized, plant-pathogenic fungi in the class Dothideomycetes. The phylogenetic affinities of the lichen mycobiont and the cultured fungus were confirmed using DNA sequence data of the mitochondrial small subunit rDNA (mtSSU), which place the lichen fungus into the Allographa clade within Graphidaceae. The mycobiont pigment was purified and characterized by spectroscopic methods. This is the first case where a rare pigment, otherwise known from non-lichenized, plant-pathogenic fungi, is produced in aposymbiotic culture of a lichen mycobiont, while, at the same time, being absent from the lichen thallus itself. Based on this finding, we discuss the previously postulated hypothesis that lichen mycobionts maintain secondary metabolic pathways of non-lichenized ancestors in their genome, while gene expression and production of metabolites is suppressed in the lichenized state due to toxicity to the photobiont. © 2018, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v17_n4_p479_Fazio http://hdl.handle.net/20.500.12110/paper_1617416X_v17_n4_p479_Fazio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Axenic culture Elsinochrome A Graphis elongata Lichen Perylenequinones
spellingShingle	Axenic culture Elsinochrome A Graphis elongata Lichen Perylenequinones Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
topic_facet	Axenic culture Elsinochrome A Graphis elongata Lichen Perylenequinones
description	We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC techniques) and is otherwise only known from one unrelated lichen and a few genera of non-lichenized, plant-pathogenic fungi in the class Dothideomycetes. The phylogenetic affinities of the lichen mycobiont and the cultured fungus were confirmed using DNA sequence data of the mitochondrial small subunit rDNA (mtSSU), which place the lichen fungus into the Allographa clade within Graphidaceae. The mycobiont pigment was purified and characterized by spectroscopic methods. This is the first case where a rare pigment, otherwise known from non-lichenized, plant-pathogenic fungi, is produced in aposymbiotic culture of a lichen mycobiont, while, at the same time, being absent from the lichen thallus itself. Based on this finding, we discuss the previously postulated hypothesis that lichen mycobionts maintain secondary metabolic pathways of non-lichenized ancestors in their genome, while gene expression and production of metabolites is suppressed in the lichenized state due to toxicity to the photobiont. © 2018, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature.
title	Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_short	Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_full	Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_fullStr	Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_full_unstemmed	Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_sort	production of the bioactive pigment elsinochrome a by a cultured mycobiont strain of the lichen graphis elongata
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v17_n4_p479_Fazio http://hdl.handle.net/20.500.12110/paper_1617416X_v17_n4_p479_Fazio
_version_	1768546227017744384

Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata

Ejemplares similares