Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system
A series of cationic drug-like substances with distinct basicity, hydrogen-bonding ability, and hydrophobicity, including three catecholamines, two beta-agonists, and thirteen beta-blockers, was successfully detected in a capillary electrophoresis system using an end-capillary coupled potentiometric...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16159306_v32_n1_p135_Bazylak http://hdl.handle.net/20.500.12110/paper_16159306_v32_n1_p135_Bazylak |
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paper:paper_16159306_v32_n1_p135_Bazylak2025-07-30T19:01:03Z Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system Aqueous-organic buffers Basic drugs Capillary electrophoresis Potentiometry Neurotransmitters Acetonitrile Alkalinity Amines Brain Capillary electrophoresis Electrochemistry Fused silica Hormones Hydrogen Hydrophobicity Liquid membranes Phosphoric acid Sensors Silica Alprenolol Analytes Aqueous-organic buffers Back ground electrolytes Baseline separations Basic drugs Beta-agonists Beta-blockers Cationic drugs Ce systems Clenbuterol Copper rods Electrophoretic separations Fused-silica capillaries Hydrogen bondings Limits of detections Linear relationships Potentiometric detections Potentiometric detectors Potentiometry Neurotransmitters Water-acetonitrile mixtures Potentiometers (electric measuring instruments) alprenolol beta adrenergic receptor blocking agent beta adrenergic receptor stimulating agent bisoprolol bufuralol carazolol carbuterol catecholamine celiprolol clenbuterol dopamine nadolol oxprenolol phosphoric acid practolol propranolol tertatolol tolamolol carbuterol ethanolamine derivative solvent analytic method article capillary electrophoresis drug determination drug structure electric potential hydrophobicity potentiometry priority journal sensitivity analysis separation technique solvent effect chemical structure chemistry methodology potentiometry reproducibility sensitivity and specificity time Adrenergic beta-Agonists Adrenergic beta-Antagonists Catecholamines Electrophoresis, Capillary Ethanolamines Hydrophobicity Molecular Structure Potentiometry Reproducibility of Results Sensitivity and Specificity Solvents Time Factors A series of cationic drug-like substances with distinct basicity, hydrogen-bonding ability, and hydrophobicity, including three catecholamines, two beta-agonists, and thirteen beta-blockers, was successfully detected in a capillary electrophoresis system using an end-capillary coupled potentiometric sensor consisting of a PVC-based liquid membrane deposited directly on a 100 μm diameter copper rod. The electrophoretic separation was performed on a 72 cm × 75 μm id uncoated fused-silica capillary with an acidic background electrolyte containing phosphoric acid in a water-acetonitrile mixture, pH* 2.8. Samples were injected electrokinetically at 5.0 kV for 10 s and a running voltage of 19.5 kV was applied. Excluding the bufuralol/ practolol pair, baseline separation of all substances was achieved in the developed CE system within 9 minutes. A linear relationship (R2 0.8752) between the sensitivity of the applied potentiometric detector and the parameter log P characterising the hydrophobicity of the analytes was demonstrated. The best observable limits of detection (LODs) were obtained for the highly hydrophobic substances, i.e. bufuralol (8.10 × 10-8 M injected concentration, S/N = 3), propranolol, alprenolol, and clenbuterol (ca. 1.10 × 1017 M). In the case of hydrophilic catecholamines and carbuterol their LODs with potentiometric detection were lowered by a factor of almost one thousand, reaching a value of 6.6 × 10-5 M. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16159306_v32_n1_p135_Bazylak http://hdl.handle.net/20.500.12110/paper_16159306_v32_n1_p135_Bazylak |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aqueous-organic buffers Basic drugs Capillary electrophoresis Potentiometry Neurotransmitters Acetonitrile Alkalinity Amines Brain Capillary electrophoresis Electrochemistry Fused silica Hormones Hydrogen Hydrophobicity Liquid membranes Phosphoric acid Sensors Silica Alprenolol Analytes Aqueous-organic buffers Back ground electrolytes Baseline separations Basic drugs Beta-agonists Beta-blockers Cationic drugs Ce systems Clenbuterol Copper rods Electrophoretic separations Fused-silica capillaries Hydrogen bondings Limits of detections Linear relationships Potentiometric detections Potentiometric detectors Potentiometry Neurotransmitters Water-acetonitrile mixtures Potentiometers (electric measuring instruments) alprenolol beta adrenergic receptor blocking agent beta adrenergic receptor stimulating agent bisoprolol bufuralol carazolol carbuterol catecholamine celiprolol clenbuterol dopamine nadolol oxprenolol phosphoric acid practolol propranolol tertatolol tolamolol carbuterol ethanolamine derivative solvent analytic method article capillary electrophoresis drug determination drug structure electric potential hydrophobicity potentiometry priority journal sensitivity analysis separation technique solvent effect chemical structure chemistry methodology potentiometry reproducibility sensitivity and specificity time Adrenergic beta-Agonists Adrenergic beta-Antagonists Catecholamines Electrophoresis, Capillary Ethanolamines Hydrophobicity Molecular Structure Potentiometry Reproducibility of Results Sensitivity and Specificity Solvents Time Factors |
| spellingShingle |
Aqueous-organic buffers Basic drugs Capillary electrophoresis Potentiometry Neurotransmitters Acetonitrile Alkalinity Amines Brain Capillary electrophoresis Electrochemistry Fused silica Hormones Hydrogen Hydrophobicity Liquid membranes Phosphoric acid Sensors Silica Alprenolol Analytes Aqueous-organic buffers Back ground electrolytes Baseline separations Basic drugs Beta-agonists Beta-blockers Cationic drugs Ce systems Clenbuterol Copper rods Electrophoretic separations Fused-silica capillaries Hydrogen bondings Limits of detections Linear relationships Potentiometric detections Potentiometric detectors Potentiometry Neurotransmitters Water-acetonitrile mixtures Potentiometers (electric measuring instruments) alprenolol beta adrenergic receptor blocking agent beta adrenergic receptor stimulating agent bisoprolol bufuralol carazolol carbuterol catecholamine celiprolol clenbuterol dopamine nadolol oxprenolol phosphoric acid practolol propranolol tertatolol tolamolol carbuterol ethanolamine derivative solvent analytic method article capillary electrophoresis drug determination drug structure electric potential hydrophobicity potentiometry priority journal sensitivity analysis separation technique solvent effect chemical structure chemistry methodology potentiometry reproducibility sensitivity and specificity time Adrenergic beta-Agonists Adrenergic beta-Antagonists Catecholamines Electrophoresis, Capillary Ethanolamines Hydrophobicity Molecular Structure Potentiometry Reproducibility of Results Sensitivity and Specificity Solvents Time Factors Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system |
| topic_facet |
Aqueous-organic buffers Basic drugs Capillary electrophoresis Potentiometry Neurotransmitters Acetonitrile Alkalinity Amines Brain Capillary electrophoresis Electrochemistry Fused silica Hormones Hydrogen Hydrophobicity Liquid membranes Phosphoric acid Sensors Silica Alprenolol Analytes Aqueous-organic buffers Back ground electrolytes Baseline separations Basic drugs Beta-agonists Beta-blockers Cationic drugs Ce systems Clenbuterol Copper rods Electrophoretic separations Fused-silica capillaries Hydrogen bondings Limits of detections Linear relationships Potentiometric detections Potentiometric detectors Potentiometry Neurotransmitters Water-acetonitrile mixtures Potentiometers (electric measuring instruments) alprenolol beta adrenergic receptor blocking agent beta adrenergic receptor stimulating agent bisoprolol bufuralol carazolol carbuterol catecholamine celiprolol clenbuterol dopamine nadolol oxprenolol phosphoric acid practolol propranolol tertatolol tolamolol carbuterol ethanolamine derivative solvent analytic method article capillary electrophoresis drug determination drug structure electric potential hydrophobicity potentiometry priority journal sensitivity analysis separation technique solvent effect chemical structure chemistry methodology potentiometry reproducibility sensitivity and specificity time Adrenergic beta-Agonists Adrenergic beta-Antagonists Catecholamines Electrophoresis, Capillary Ethanolamines Hydrophobicity Molecular Structure Potentiometry Reproducibility of Results Sensitivity and Specificity Solvents Time Factors |
| description |
A series of cationic drug-like substances with distinct basicity, hydrogen-bonding ability, and hydrophobicity, including three catecholamines, two beta-agonists, and thirteen beta-blockers, was successfully detected in a capillary electrophoresis system using an end-capillary coupled potentiometric sensor consisting of a PVC-based liquid membrane deposited directly on a 100 μm diameter copper rod. The electrophoretic separation was performed on a 72 cm × 75 μm id uncoated fused-silica capillary with an acidic background electrolyte containing phosphoric acid in a water-acetonitrile mixture, pH* 2.8. Samples were injected electrokinetically at 5.0 kV for 10 s and a running voltage of 19.5 kV was applied. Excluding the bufuralol/ practolol pair, baseline separation of all substances was achieved in the developed CE system within 9 minutes. A linear relationship (R2 0.8752) between the sensitivity of the applied potentiometric detector and the parameter log P characterising the hydrophobicity of the analytes was demonstrated. The best observable limits of detection (LODs) were obtained for the highly hydrophobic substances, i.e. bufuralol (8.10 × 10-8 M injected concentration, S/N = 3), propranolol, alprenolol, and clenbuterol (ca. 1.10 × 1017 M). In the case of hydrophilic catecholamines and carbuterol their LODs with potentiometric detection were lowered by a factor of almost one thousand, reaching a value of 6.6 × 10-5 M. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| title |
Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system |
| title_short |
Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system |
| title_full |
Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system |
| title_fullStr |
Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system |
| title_full_unstemmed |
Hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system |
| title_sort |
hydrophobicity-aided potentiometric detection of catecholamines, beta-agonists, and beta-blockers in a mixed-solvent capillary electrophoresis system |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16159306_v32_n1_p135_Bazylak http://hdl.handle.net/20.500.12110/paper_16159306_v32_n1_p135_Bazylak |
| _version_ |
1840324265446998016 |