Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene...
| Publicado: |
2011
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15904261_v61_n3_p575_Tranchida http://hdl.handle.net/20.500.12110/paper_15904261_v61_n3_p575_Tranchida |
| Aporte de: |
| id |
paper:paper_15904261_v61_n3_p575_Tranchida |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_15904261_v61_n3_p575_Tranchida2023-06-08T16:24:54Z Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae 16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15904261_v61_n3_p575_Tranchida http://hdl.handle.net/20.500.12110/paper_15904261_v61_n3_p575_Tranchida |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria |
| spellingShingle |
16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae |
| topic_facet |
16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria |
| description |
Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010. |
| title |
Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae |
| title_short |
Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae |
| title_full |
Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae |
| title_fullStr |
Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae |
| title_full_unstemmed |
Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae |
| title_sort |
isolation, characterization and evaluation of mosquitocidal activity of lysinibacillus strains obtained from culex pipiens larvae |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15904261_v61_n3_p575_Tranchida http://hdl.handle.net/20.500.12110/paper_15904261_v61_n3_p575_Tranchida |
| _version_ |
1768546416656908288 |