VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages
The spontaneous non obese diabetic (NOD) mouse model of Sjögren's syndrome provides a valuable tool to study the onset and progression of both autoimmune response and secretory dysfunction. Vasoactive intestinal peptide (VIP) is a neuro and immunopeptide with prosecretory effect in salivary gla...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Publicado: |
2007
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15675769_v7_n10_p1343_Larocca http://hdl.handle.net/20.500.12110/paper_15675769_v7_n10_p1343_Larocca |
| Aporte de: |
| id |
paper:paper_15675769_v7_n10_p1343_Larocca |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_15675769_v7_n10_p1343_Larocca2023-06-08T16:24:08Z VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages Larocca, Luciana Calafat, Mario José Roca, Valeria Inés Franchi, Ana María Pérez Leirós, Claudia IL-10 Macrophages Nitric oxide NOD mice PGE2 VIP interleukin 10 interleukin 10 antibody interleukin 12 lipopolysaccharide monoclonal antibody nitric oxide nitrite prostaglandin E2 prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal cell animal experiment animal model antiinflammatory activity article autoimmune disease cell secretion controlled study cytokine production drug mechanism female immunopharmacology mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal salivary gland Sjoegren syndrome Animals Anti-Inflammatory Agents Dinoprostone Female Interferon Type II Interleukin-10 Interleukin-12 Lipopolysaccharides Macrophages, Peritoneal Mice Mice, Inbred BALB C Mice, Inbred NOD Nitric Oxide Nitrites Vasoactive Intestinal Peptide The spontaneous non obese diabetic (NOD) mouse model of Sjögren's syndrome provides a valuable tool to study the onset and progression of both autoimmune response and secretory dysfunction. Vasoactive intestinal peptide (VIP) is a neuro and immunopeptide with prosecretory effect in salivary glands and anti-inflammatory actions in various models of autoimmune disease. Our purpose was to analyze the response of peritoneal macrophages to an inflammatory stimulus during the decline of salivary secretion in NOD mice and the potential anti-inflammatory effect of VIP. We present evidence of an increased nitric oxide production by peritoneal macrophages of NOD mice in basal and lipopolysaccharide (LPS) + IFN-γ-stimulated conditions and a lower IL-10 response to LPS compared with normal BALB/c mice. VIP inhibited LPS-induced TNF-α, IL-12 and nitrites accumulation in NOD macrophages while it increased IL-10 production. VIP effect was prevented by an anti-IL-10 monoclonal antibody and it showed an additive effect on exogenously added IL-10 only in NOD mice. The inhibitory effect of VIP-induced IL-10 on nitrites was mediated by COX metabolites mostly in NOD cells as indomethacine inhibited both the increase in IL-10 and the reduction of nitrites exerted by VIP. We conclude that both PGE2 and VIP inhibit nitric oxide production and increase IL-10 induced by LPS in NOD macrophages and VIP effect is mediated through an increase of COX metabolites and IL-10. © 2007 Elsevier B.V. All rights reserved. Fil:Larocca, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calafat, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roca, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Franchi, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15675769_v7_n10_p1343_Larocca http://hdl.handle.net/20.500.12110/paper_15675769_v7_n10_p1343_Larocca |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
IL-10 Macrophages Nitric oxide NOD mice PGE2 VIP interleukin 10 interleukin 10 antibody interleukin 12 lipopolysaccharide monoclonal antibody nitric oxide nitrite prostaglandin E2 prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal cell animal experiment animal model antiinflammatory activity article autoimmune disease cell secretion controlled study cytokine production drug mechanism female immunopharmacology mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal salivary gland Sjoegren syndrome Animals Anti-Inflammatory Agents Dinoprostone Female Interferon Type II Interleukin-10 Interleukin-12 Lipopolysaccharides Macrophages, Peritoneal Mice Mice, Inbred BALB C Mice, Inbred NOD Nitric Oxide Nitrites Vasoactive Intestinal Peptide |
| spellingShingle |
IL-10 Macrophages Nitric oxide NOD mice PGE2 VIP interleukin 10 interleukin 10 antibody interleukin 12 lipopolysaccharide monoclonal antibody nitric oxide nitrite prostaglandin E2 prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal cell animal experiment animal model antiinflammatory activity article autoimmune disease cell secretion controlled study cytokine production drug mechanism female immunopharmacology mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal salivary gland Sjoegren syndrome Animals Anti-Inflammatory Agents Dinoprostone Female Interferon Type II Interleukin-10 Interleukin-12 Lipopolysaccharides Macrophages, Peritoneal Mice Mice, Inbred BALB C Mice, Inbred NOD Nitric Oxide Nitrites Vasoactive Intestinal Peptide Larocca, Luciana Calafat, Mario José Roca, Valeria Inés Franchi, Ana María Pérez Leirós, Claudia VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages |
| topic_facet |
IL-10 Macrophages Nitric oxide NOD mice PGE2 VIP interleukin 10 interleukin 10 antibody interleukin 12 lipopolysaccharide monoclonal antibody nitric oxide nitrite prostaglandin E2 prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal cell animal experiment animal model antiinflammatory activity article autoimmune disease cell secretion controlled study cytokine production drug mechanism female immunopharmacology mouse nonhuman nonobese diabetic mouse peritoneum macrophage priority journal salivary gland Sjoegren syndrome Animals Anti-Inflammatory Agents Dinoprostone Female Interferon Type II Interleukin-10 Interleukin-12 Lipopolysaccharides Macrophages, Peritoneal Mice Mice, Inbred BALB C Mice, Inbred NOD Nitric Oxide Nitrites Vasoactive Intestinal Peptide |
| description |
The spontaneous non obese diabetic (NOD) mouse model of Sjögren's syndrome provides a valuable tool to study the onset and progression of both autoimmune response and secretory dysfunction. Vasoactive intestinal peptide (VIP) is a neuro and immunopeptide with prosecretory effect in salivary glands and anti-inflammatory actions in various models of autoimmune disease. Our purpose was to analyze the response of peritoneal macrophages to an inflammatory stimulus during the decline of salivary secretion in NOD mice and the potential anti-inflammatory effect of VIP. We present evidence of an increased nitric oxide production by peritoneal macrophages of NOD mice in basal and lipopolysaccharide (LPS) + IFN-γ-stimulated conditions and a lower IL-10 response to LPS compared with normal BALB/c mice. VIP inhibited LPS-induced TNF-α, IL-12 and nitrites accumulation in NOD macrophages while it increased IL-10 production. VIP effect was prevented by an anti-IL-10 monoclonal antibody and it showed an additive effect on exogenously added IL-10 only in NOD mice. The inhibitory effect of VIP-induced IL-10 on nitrites was mediated by COX metabolites mostly in NOD cells as indomethacine inhibited both the increase in IL-10 and the reduction of nitrites exerted by VIP. We conclude that both PGE2 and VIP inhibit nitric oxide production and increase IL-10 induced by LPS in NOD macrophages and VIP effect is mediated through an increase of COX metabolites and IL-10. © 2007 Elsevier B.V. All rights reserved. |
| author |
Larocca, Luciana Calafat, Mario José Roca, Valeria Inés Franchi, Ana María Pérez Leirós, Claudia |
| author_facet |
Larocca, Luciana Calafat, Mario José Roca, Valeria Inés Franchi, Ana María Pérez Leirós, Claudia |
| author_sort |
Larocca, Luciana |
| title |
VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages |
| title_short |
VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages |
| title_full |
VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages |
| title_fullStr |
VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages |
| title_full_unstemmed |
VIP limits LPS-induced nitric oxide production through IL-10 in NOD mice macrophages |
| title_sort |
vip limits lps-induced nitric oxide production through il-10 in nod mice macrophages |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15675769_v7_n10_p1343_Larocca http://hdl.handle.net/20.500.12110/paper_15675769_v7_n10_p1343_Larocca |
| work_keys_str_mv |
AT laroccaluciana viplimitslpsinducednitricoxideproductionthroughil10innodmicemacrophages AT calafatmariojose viplimitslpsinducednitricoxideproductionthroughil10innodmicemacrophages AT rocavaleriaines viplimitslpsinducednitricoxideproductionthroughil10innodmicemacrophages AT franchianamaria viplimitslpsinducednitricoxideproductionthroughil10innodmicemacrophages AT perezleirosclaudia viplimitslpsinducednitricoxideproductionthroughil10innodmicemacrophages |
| _version_ |
1768546086779092992 |