Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modificatio...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15675394_v121_n_p169_Peinetti http://hdl.handle.net/20.500.12110/paper_15675394_v121_n_p169_Peinetti |
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paper:paper_15675394_v121_n_p169_Peinetti2023-06-08T16:24:05Z Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array Alumina Fiber optic sensors Gold Metal nanoparticles Nanoparticles Oligonucleotides Surface treatment Complementary strand Confined nanoparticles Electrochemical behaviors Electrochemical response Impedance based sensors Molecular recognition properties Nano-channel arrays Oligonucleotide conjugates DNA aluminum oxide DNA element gold nanoparticle nanochannel sulfur sulfur derivative DNA gold immobilized nucleic acid metal nanoparticle nanomaterial Article clinical evaluation concentration process conjugation DNA probe DNA sequence DNA strand electrochemical analysis electrochemical impedance spectroscopy immobilization impedance molecular recognition nanochannel array particle size sensitivity analysis surface area chemistry electrochemical analysis electrode genetic procedures nucleic acid hybridization porosity procedures Aluminum Oxide Biosensing Techniques DNA Electrochemical Techniques Electrodes Gold Immobilized Nucleic Acids Metal Nanoparticles Nanostructures Nucleic Acid Hybridization Porosity Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modification strategies on the interplay between the meso-organization and the molecular recognition properties of a 27-mer DNA strand. This DNA strand is functionalized with different sulfur-containing moieties and immobilized on 2 nm gold nanoparticles confined on a nanoporous alumina, working the whole system as an electrode array. Surface coverages were determined by EXAFS and the performance as recognition elements for impedance-based sensors is evaluated. Our results prove that low DNA coverages on the confined nanoparticles prompt to a more sensitive response, showing the relevance in avoiding the DNA strand overcrowding. The system was able to determine a concentration as low as 100 pM of the complementary strand, thus introducing the foundations for the construction of label-free genosensors at the nanometer scale. © 2017 Elsevier B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15675394_v121_n_p169_Peinetti http://hdl.handle.net/20.500.12110/paper_15675394_v121_n_p169_Peinetti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alumina Fiber optic sensors Gold Metal nanoparticles Nanoparticles Oligonucleotides Surface treatment Complementary strand Confined nanoparticles Electrochemical behaviors Electrochemical response Impedance based sensors Molecular recognition properties Nano-channel arrays Oligonucleotide conjugates DNA aluminum oxide DNA element gold nanoparticle nanochannel sulfur sulfur derivative DNA gold immobilized nucleic acid metal nanoparticle nanomaterial Article clinical evaluation concentration process conjugation DNA probe DNA sequence DNA strand electrochemical analysis electrochemical impedance spectroscopy immobilization impedance molecular recognition nanochannel array particle size sensitivity analysis surface area chemistry electrochemical analysis electrode genetic procedures nucleic acid hybridization porosity procedures Aluminum Oxide Biosensing Techniques DNA Electrochemical Techniques Electrodes Gold Immobilized Nucleic Acids Metal Nanoparticles Nanostructures Nucleic Acid Hybridization Porosity |
| spellingShingle |
Alumina Fiber optic sensors Gold Metal nanoparticles Nanoparticles Oligonucleotides Surface treatment Complementary strand Confined nanoparticles Electrochemical behaviors Electrochemical response Impedance based sensors Molecular recognition properties Nano-channel arrays Oligonucleotide conjugates DNA aluminum oxide DNA element gold nanoparticle nanochannel sulfur sulfur derivative DNA gold immobilized nucleic acid metal nanoparticle nanomaterial Article clinical evaluation concentration process conjugation DNA probe DNA sequence DNA strand electrochemical analysis electrochemical impedance spectroscopy immobilization impedance molecular recognition nanochannel array particle size sensitivity analysis surface area chemistry electrochemical analysis electrode genetic procedures nucleic acid hybridization porosity procedures Aluminum Oxide Biosensing Techniques DNA Electrochemical Techniques Electrodes Gold Immobilized Nucleic Acids Metal Nanoparticles Nanostructures Nucleic Acid Hybridization Porosity Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array |
| topic_facet |
Alumina Fiber optic sensors Gold Metal nanoparticles Nanoparticles Oligonucleotides Surface treatment Complementary strand Confined nanoparticles Electrochemical behaviors Electrochemical response Impedance based sensors Molecular recognition properties Nano-channel arrays Oligonucleotide conjugates DNA aluminum oxide DNA element gold nanoparticle nanochannel sulfur sulfur derivative DNA gold immobilized nucleic acid metal nanoparticle nanomaterial Article clinical evaluation concentration process conjugation DNA probe DNA sequence DNA strand electrochemical analysis electrochemical impedance spectroscopy immobilization impedance molecular recognition nanochannel array particle size sensitivity analysis surface area chemistry electrochemical analysis electrode genetic procedures nucleic acid hybridization porosity procedures Aluminum Oxide Biosensing Techniques DNA Electrochemical Techniques Electrodes Gold Immobilized Nucleic Acids Metal Nanoparticles Nanostructures Nucleic Acid Hybridization Porosity |
| description |
Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modification strategies on the interplay between the meso-organization and the molecular recognition properties of a 27-mer DNA strand. This DNA strand is functionalized with different sulfur-containing moieties and immobilized on 2 nm gold nanoparticles confined on a nanoporous alumina, working the whole system as an electrode array. Surface coverages were determined by EXAFS and the performance as recognition elements for impedance-based sensors is evaluated. Our results prove that low DNA coverages on the confined nanoparticles prompt to a more sensitive response, showing the relevance in avoiding the DNA strand overcrowding. The system was able to determine a concentration as low as 100 pM of the complementary strand, thus introducing the foundations for the construction of label-free genosensors at the nanometer scale. © 2017 Elsevier B.V. |
| title |
Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array |
| title_short |
Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array |
| title_full |
Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array |
| title_fullStr |
Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array |
| title_full_unstemmed |
Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array |
| title_sort |
characterization and electrochemical response of dna functionalized 2 nm gold nanoparticles confined in a nanochannel array |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15675394_v121_n_p169_Peinetti http://hdl.handle.net/20.500.12110/paper_15675394_v121_n_p169_Peinetti |
| _version_ |
1768545573490655232 |