Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco

The patterns of genetic structure in natural populations provide essential information for the improvement of pest management strategies including those targeting arthropod vectors of human diseases. We analyzed the patterns of fine-scale genetic structure in Triatoma infestans in a well-defined rur...

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Autores principales:	Piccinali, Romina Valeria, Gurtler, Ricardo Esteban
Publicado:	2015
Materias:	Genetic structure Gran Chaco Microsatellites Triatoma infestans Argentina Article controlled study domestic animal effective population size gene cluster gene linkage disequilibrium genotype longitudinal study microsatellite marker morphometrics nonhuman null allele pest infestation population genetic structure priority journal rural area vector control animal Chagas disease disease carrier DNA sequence ecosystem female gene gene frequency genetics human insect control male transmission Triatoma Arthropoda Hexapoda microsatellite DNA Animals Chagas Disease Ecosystem Female Gene Frequency Genes, Insect Genotype Humans Insect Control Insect Vectors Linkage Disequilibrium Longitudinal Studies Male Microsatellite Repeats Sequence Analysis, DNA
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v34_n_p143_Piccinali http://hdl.handle.net/20.500.12110/paper_15671348_v34_n_p143_Piccinali
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15671348_v34_n_p143_Piccinali
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spelling	paper:paper_15671348_v34_n_p143_Piccinali2023-06-08T16:23:58Z Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco Piccinali, Romina Valeria Gurtler, Ricardo Esteban Genetic structure Gran Chaco Microsatellites Triatoma infestans Argentina Article controlled study domestic animal effective population size gene cluster gene linkage disequilibrium genotype longitudinal study microsatellite marker morphometrics nonhuman null allele pest infestation population genetic structure priority journal rural area Triatoma infestans vector control animal Chagas disease disease carrier DNA sequence ecosystem female gene gene frequency genetics human insect control male transmission Triatoma Arthropoda Hexapoda Triatoma infestans microsatellite DNA Animals Chagas Disease Ecosystem Female Gene Frequency Genes, Insect Genotype Humans Insect Control Insect Vectors Linkage Disequilibrium Longitudinal Studies Male Microsatellite Repeats Sequence Analysis, DNA Triatoma The patterns of genetic structure in natural populations provide essential information for the improvement of pest management strategies including those targeting arthropod vectors of human diseases. We analyzed the patterns of fine-scale genetic structure in Triatoma infestans in a well-defined rural area close to Pampa del Indio, in the Argentine Arid-Humid Chaco transition, where a longitudinal study on house infestation and wing geometric morphometry is being conducted since 2007. A total of 228 insects collected in 16 domestic and peridomestic sites from two rural communities was genotyped for 10 microsatellite loci and analyzed. We did not find departures from Hardy-Weinberg expectations in collection sites, with three exceptions probably due to null alleles and substructuring. Domestic sites were more variable than peridomestic sites suggesting the presence of older bug populations in domestic sites or higher effective population sizes. Significant genetic structure was detected using F-statistics, a discriminant analysis of principal components and Bayesian clustering algorithms in an area of only 6.32km2. Microsatellite markers detected population structuring at a finer geographic scale (180-6300m) than a previous study based on wing geometric morphometry (>4000m). The spatial distribution of genetic variability was more properly explained by a hierarchical island than by an isolation-by-distance model. This study illustrates that, despite more than a decade without vector control interventions enhancing differentiation, genetic structure can be detected in T. infestans populations, particularly applying spatial information. This supports the potential of genetic studies to provide key information for hypothesis testing of the origins of house reinfestation. © 2015 Elsevier B.V. Fil:Piccinali, R.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v34_n_p143_Piccinali http://hdl.handle.net/20.500.12110/paper_15671348_v34_n_p143_Piccinali
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Genetic structure Gran Chaco Microsatellites Triatoma infestans Argentina Article controlled study domestic animal effective population size gene cluster gene linkage disequilibrium genotype longitudinal study microsatellite marker morphometrics nonhuman null allele pest infestation population genetic structure priority journal rural area Triatoma infestans vector control animal Chagas disease disease carrier DNA sequence ecosystem female gene gene frequency genetics human insect control male transmission Triatoma Arthropoda Hexapoda Triatoma infestans microsatellite DNA Animals Chagas Disease Ecosystem Female Gene Frequency Genes, Insect Genotype Humans Insect Control Insect Vectors Linkage Disequilibrium Longitudinal Studies Male Microsatellite Repeats Sequence Analysis, DNA Triatoma
spellingShingle	Genetic structure Gran Chaco Microsatellites Triatoma infestans Argentina Article controlled study domestic animal effective population size gene cluster gene linkage disequilibrium genotype longitudinal study microsatellite marker morphometrics nonhuman null allele pest infestation population genetic structure priority journal rural area Triatoma infestans vector control animal Chagas disease disease carrier DNA sequence ecosystem female gene gene frequency genetics human insect control male transmission Triatoma Arthropoda Hexapoda Triatoma infestans microsatellite DNA Animals Chagas Disease Ecosystem Female Gene Frequency Genes, Insect Genotype Humans Insect Control Insect Vectors Linkage Disequilibrium Longitudinal Studies Male Microsatellite Repeats Sequence Analysis, DNA Triatoma Piccinali, Romina Valeria Gurtler, Ricardo Esteban Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco
topic_facet	Genetic structure Gran Chaco Microsatellites Triatoma infestans Argentina Article controlled study domestic animal effective population size gene cluster gene linkage disequilibrium genotype longitudinal study microsatellite marker morphometrics nonhuman null allele pest infestation population genetic structure priority journal rural area Triatoma infestans vector control animal Chagas disease disease carrier DNA sequence ecosystem female gene gene frequency genetics human insect control male transmission Triatoma Arthropoda Hexapoda Triatoma infestans microsatellite DNA Animals Chagas Disease Ecosystem Female Gene Frequency Genes, Insect Genotype Humans Insect Control Insect Vectors Linkage Disequilibrium Longitudinal Studies Male Microsatellite Repeats Sequence Analysis, DNA Triatoma
description	The patterns of genetic structure in natural populations provide essential information for the improvement of pest management strategies including those targeting arthropod vectors of human diseases. We analyzed the patterns of fine-scale genetic structure in Triatoma infestans in a well-defined rural area close to Pampa del Indio, in the Argentine Arid-Humid Chaco transition, where a longitudinal study on house infestation and wing geometric morphometry is being conducted since 2007. A total of 228 insects collected in 16 domestic and peridomestic sites from two rural communities was genotyped for 10 microsatellite loci and analyzed. We did not find departures from Hardy-Weinberg expectations in collection sites, with three exceptions probably due to null alleles and substructuring. Domestic sites were more variable than peridomestic sites suggesting the presence of older bug populations in domestic sites or higher effective population sizes. Significant genetic structure was detected using F-statistics, a discriminant analysis of principal components and Bayesian clustering algorithms in an area of only 6.32km2. Microsatellite markers detected population structuring at a finer geographic scale (180-6300m) than a previous study based on wing geometric morphometry (>4000m). The spatial distribution of genetic variability was more properly explained by a hierarchical island than by an isolation-by-distance model. This study illustrates that, despite more than a decade without vector control interventions enhancing differentiation, genetic structure can be detected in T. infestans populations, particularly applying spatial information. This supports the potential of genetic studies to provide key information for hypothesis testing of the origins of house reinfestation. © 2015 Elsevier B.V.
author	Piccinali, Romina Valeria Gurtler, Ricardo Esteban
author_facet	Piccinali, Romina Valeria Gurtler, Ricardo Esteban
author_sort	Piccinali, Romina Valeria
title	Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco
title_short	Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco
title_full	Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco
title_fullStr	Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco
title_full_unstemmed	Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco
title_sort	fine-scale genetic structure of triatoma infestans in the argentine chaco
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v34_n_p143_Piccinali http://hdl.handle.net/20.500.12110/paper_15671348_v34_n_p143_Piccinali
work_keys_str_mv	AT piccinalirominavaleria finescalegeneticstructureoftriatomainfestansintheargentinechaco AT gurtlerricardoesteban finescalegeneticstructureoftriatomainfestansintheargentinechaco
_version_	1768543626692919296

Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco

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