The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco

The role of rodents in the sylvatic transmission of Trypanosoma cruzi has seldom been investigated using parasitological and molecular methods. We assessed the occurrence of T. cruzi in wild small rodents from Pampa del Indio, in the Argentinean Chaco, and identified the taxonomic status of positive...

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Autores principales:	Orozco, Maria Marcela, Piccinali, Romina Valeria, Enriquez, Gustavo Fabian, Cardinal, Marta Victoria, Gurtler, Ricardo Esteban
Publicado:	2014
Materias:	Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi cytochrome b kinetoplast DNA minicircle DNA protozoal DNA Akodon montensis Akodon toba Argentina article Calomys callosus controlled study gene sequence Graomys chacoensis host parasite interaction kinetoplast Necromys lasiurus nonhuman nucleotide sequence Oligoryzomys chacoensis Oligoryzomys nigripes parasite prevalence parasite virulence phylogeny polymerase chain reaction priority journal rice rat species identification trypanosomiasis xenodiagnosis Article Chagas disease cytochrome b gene DNA sequence habitat structure infection rate limit of detection parasite transmission wild animal Animals Chagas Disease Disease Reservoirs
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v22_n_p12_Orozco http://hdl.handle.net/20.500.12110/paper_15671348_v22_n_p12_Orozco
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_15671348_v22_n_p12_Orozco2023-06-08T16:23:57Z The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco Orozco, Maria Marcela Piccinali, Romina Valeria Enriquez, Gustavo Fabian Cardinal, Marta Victoria Gurtler, Ricardo Esteban Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi cytochrome b kinetoplast DNA minicircle DNA cytochrome b kinetoplast DNA protozoal DNA Akodon montensis Akodon toba Argentina article Calomys callosus controlled study gene sequence Graomys chacoensis host parasite interaction kinetoplast Necromys lasiurus nonhuman nucleotide sequence Oligoryzomys chacoensis Oligoryzomys nigripes parasite prevalence parasite virulence phylogeny polymerase chain reaction priority journal rice rat Sigmodontinae species identification Trypanosoma cruzi trypanosomiasis xenodiagnosis Article Chagas disease cytochrome b gene DNA sequence habitat structure host parasite interaction infection rate limit of detection nucleotide sequence parasite transmission wild animal Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi Animals Argentina Chagas Disease Disease Reservoirs Sigmodontinae Trypanosoma cruzi The role of rodents in the sylvatic transmission of Trypanosoma cruzi has seldom been investigated using parasitological and molecular methods. We assessed the occurrence of T. cruzi in wild small rodents from Pampa del Indio, in the Argentinean Chaco, and identified the taxonomic status of positive rodents by sequencing a fragment of cytochrome b gene (cytb) and performing BLAST searches and phylogenetic analyses. A total of 176 Sigmodontinae rodents was captured in six surveys using 5425 trap-nights in a wide range of sylvatic habitats between 2009 and 2011. Host infection was determined by xenodiagnosis and by polymerase chain reaction amplification of the hyper-variable region of kinetoplast DNA minicircles of T. cruzi (kDNA-PCR) from blood samples. None of the 176 rodents examined was xenodiagnosis-positive. The prevalence of infection determined by kDNA-PCR from blood samples was 16.2% (95% confidence interval, 10.1-21.9%). Half of the infections detected by kDNA-PCR were confirmed by nuclear satellite DNA-PCR or by kDNA-PCR of the rectal contents of xenodiagnostic bugs. The 24 positive specimens were assigned to eight species, providing the first records of T. cruzi in Akodon montensis, Akodon toba, Graomys chacoensis, and Oligoryzomys chacoensis. The occurrence of T. cruzi infection in Oligoryzomys nigripes, Calomys callosus, Necromys lasiurus and Oecomys sp. (most probably Oecomys mamorae) from the Gran Chaco is also reported for the first time. Although sigmodontine rodents were frequently infected, the intensity of bug rectal infection with T. cruzi was below the detection limit of xenodiagnosis (subpatent infectiousness to bugs), indicating they had a low reservoir host competence. © 2013 Elsevier B.V. Fil:Orozco, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piccinali, R.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Enriquez, G.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cardinal, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v22_n_p12_Orozco http://hdl.handle.net/20.500.12110/paper_15671348_v22_n_p12_Orozco
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi cytochrome b kinetoplast DNA minicircle DNA cytochrome b kinetoplast DNA protozoal DNA Akodon montensis Akodon toba Argentina article Calomys callosus controlled study gene sequence Graomys chacoensis host parasite interaction kinetoplast Necromys lasiurus nonhuman nucleotide sequence Oligoryzomys chacoensis Oligoryzomys nigripes parasite prevalence parasite virulence phylogeny polymerase chain reaction priority journal rice rat Sigmodontinae species identification Trypanosoma cruzi trypanosomiasis xenodiagnosis Article Chagas disease cytochrome b gene DNA sequence habitat structure host parasite interaction infection rate limit of detection nucleotide sequence parasite transmission wild animal Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi Animals Argentina Chagas Disease Disease Reservoirs Sigmodontinae Trypanosoma cruzi
spellingShingle	Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi cytochrome b kinetoplast DNA minicircle DNA cytochrome b kinetoplast DNA protozoal DNA Akodon montensis Akodon toba Argentina article Calomys callosus controlled study gene sequence Graomys chacoensis host parasite interaction kinetoplast Necromys lasiurus nonhuman nucleotide sequence Oligoryzomys chacoensis Oligoryzomys nigripes parasite prevalence parasite virulence phylogeny polymerase chain reaction priority journal rice rat Sigmodontinae species identification Trypanosoma cruzi trypanosomiasis xenodiagnosis Article Chagas disease cytochrome b gene DNA sequence habitat structure host parasite interaction infection rate limit of detection nucleotide sequence parasite transmission wild animal Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi Animals Argentina Chagas Disease Disease Reservoirs Sigmodontinae Trypanosoma cruzi Orozco, Maria Marcela Piccinali, Romina Valeria Enriquez, Gustavo Fabian Cardinal, Marta Victoria Gurtler, Ricardo Esteban The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco
topic_facet	Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi cytochrome b kinetoplast DNA minicircle DNA cytochrome b kinetoplast DNA protozoal DNA Akodon montensis Akodon toba Argentina article Calomys callosus controlled study gene sequence Graomys chacoensis host parasite interaction kinetoplast Necromys lasiurus nonhuman nucleotide sequence Oligoryzomys chacoensis Oligoryzomys nigripes parasite prevalence parasite virulence phylogeny polymerase chain reaction priority journal rice rat Sigmodontinae species identification Trypanosoma cruzi trypanosomiasis xenodiagnosis Article Chagas disease cytochrome b gene DNA sequence habitat structure host parasite interaction infection rate limit of detection nucleotide sequence parasite transmission wild animal Molecular diagnosis Molecular identification Reservoir competence Rodents Sigmodontinae Trypanosoma cruzi Animals Argentina Chagas Disease Disease Reservoirs Sigmodontinae Trypanosoma cruzi
description	The role of rodents in the sylvatic transmission of Trypanosoma cruzi has seldom been investigated using parasitological and molecular methods. We assessed the occurrence of T. cruzi in wild small rodents from Pampa del Indio, in the Argentinean Chaco, and identified the taxonomic status of positive rodents by sequencing a fragment of cytochrome b gene (cytb) and performing BLAST searches and phylogenetic analyses. A total of 176 Sigmodontinae rodents was captured in six surveys using 5425 trap-nights in a wide range of sylvatic habitats between 2009 and 2011. Host infection was determined by xenodiagnosis and by polymerase chain reaction amplification of the hyper-variable region of kinetoplast DNA minicircles of T. cruzi (kDNA-PCR) from blood samples. None of the 176 rodents examined was xenodiagnosis-positive. The prevalence of infection determined by kDNA-PCR from blood samples was 16.2% (95% confidence interval, 10.1-21.9%). Half of the infections detected by kDNA-PCR were confirmed by nuclear satellite DNA-PCR or by kDNA-PCR of the rectal contents of xenodiagnostic bugs. The 24 positive specimens were assigned to eight species, providing the first records of T. cruzi in Akodon montensis, Akodon toba, Graomys chacoensis, and Oligoryzomys chacoensis. The occurrence of T. cruzi infection in Oligoryzomys nigripes, Calomys callosus, Necromys lasiurus and Oecomys sp. (most probably Oecomys mamorae) from the Gran Chaco is also reported for the first time. Although sigmodontine rodents were frequently infected, the intensity of bug rectal infection with T. cruzi was below the detection limit of xenodiagnosis (subpatent infectiousness to bugs), indicating they had a low reservoir host competence. © 2013 Elsevier B.V.
author	Orozco, Maria Marcela Piccinali, Romina Valeria Enriquez, Gustavo Fabian Cardinal, Marta Victoria Gurtler, Ricardo Esteban
author_facet	Orozco, Maria Marcela Piccinali, Romina Valeria Enriquez, Gustavo Fabian Cardinal, Marta Victoria Gurtler, Ricardo Esteban
author_sort	Orozco, Maria Marcela
title	The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco
title_short	The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco
title_full	The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco
title_fullStr	The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco
title_full_unstemmed	The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco
title_sort	role of sigmodontine rodents as sylvatic hosts of trypanosoma cruzi in the argentinean chaco
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v22_n_p12_Orozco http://hdl.handle.net/20.500.12110/paper_15671348_v22_n_p12_Orozco
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The role of sigmodontine rodents as sylvatic hosts of Trypanosoma cruzi in the Argentinean Chaco

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