Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populatio...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v11_n5_p895_Piccinali http://hdl.handle.net/20.500.12110/paper_15671348_v11_n5_p895_Piccinali |
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paper:paper_15671348_v11_n5_p895_Piccinali2023-06-08T16:23:57Z Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco Piccinali, Romina Valeria Marcet, Paula Lorena Ceballos, Leonardo Adrián Gurtler, Ricardo Esteban Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populations in the recolonization process of insecticide-treated houses. The finding of a population of sylvatic melanic T. infestans (dark morphs) in the Argentinean dry Chaco at 7. km from a peridomestic bug population of typical coloration gave us the opportunity to test both questions simultaneously by employing phylogenetic and population genetic approaches. For this purpose we analyzed sylvatic and peridomestic bugs using sequence-based mitochondrial and nuclear markers (mtCOI and ITS-1) and microsatellites. Sylvatic bugs were confirmed to be T. infestans and not hybrids, and showed high levels of genetic variability and departures from neutral expectations for mtCOI variation. New ITS-1 and mtCOI haplotypes were recorded, as well as haplotypes shared with peridomestic and/or domestic bugs from previous records. The peridomestic population was invariant for ITS-1 and mtCOI, but showed variability for microsatellites and signatures of a population bottleneck, probably due to a limited number of founders. Phylogenetic analyses were consistent with the presence of ancestral haplotypes in sylvatic bugs. According to F-statistics and assignment methods there was a significant differentiation between sylvatic and peridomestic bugs and gene flow was low and asymmetric, with more bugs moving from the peridomicile to the sylvatic environment. These results support the hypothesis of the Chaco region as the area of origin of T. infestans, and a limited role of sylvatic melanic T. infestans in peridomestic infestation in the Argentinean Chaco. © 2011 Elsevier B.V. Fil:Piccinali, R.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marcet, P.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v11_n5_p895_Piccinali http://hdl.handle.net/20.500.12110/paper_15671348_v11_n5_p895_Piccinali |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans |
| spellingShingle |
Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans Piccinali, Romina Valeria Marcet, Paula Lorena Ceballos, Leonardo Adrián Gurtler, Ricardo Esteban Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco |
| topic_facet |
Dark morphs Epidemiological relevance Evolutionary history Gene flow Triatoma infestans mitochondrial protein nuclear protein protein ITS 1 protein mtCOI unclassified drug article bottleneck population controlled study domestic species environmental factor gene gene flow gene sequence genetic variability haplotype insect genetics ITS 1 gene microsatellite marker mtCOI gene nonhuman nucleotide sequence phylogeny population genetics priority journal Triatoma infestans Animals Argentina Base Sequence DNA, Intergenic Electron Transport Complex IV Gene Expression Regulation Gene Flow Genetic Variation Haplotypes Mitochondria Phylogeny Pigments, Biological Triatoma Triatoma infestans |
| description |
The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populations in the recolonization process of insecticide-treated houses. The finding of a population of sylvatic melanic T. infestans (dark morphs) in the Argentinean dry Chaco at 7. km from a peridomestic bug population of typical coloration gave us the opportunity to test both questions simultaneously by employing phylogenetic and population genetic approaches. For this purpose we analyzed sylvatic and peridomestic bugs using sequence-based mitochondrial and nuclear markers (mtCOI and ITS-1) and microsatellites. Sylvatic bugs were confirmed to be T. infestans and not hybrids, and showed high levels of genetic variability and departures from neutral expectations for mtCOI variation. New ITS-1 and mtCOI haplotypes were recorded, as well as haplotypes shared with peridomestic and/or domestic bugs from previous records. The peridomestic population was invariant for ITS-1 and mtCOI, but showed variability for microsatellites and signatures of a population bottleneck, probably due to a limited number of founders. Phylogenetic analyses were consistent with the presence of ancestral haplotypes in sylvatic bugs. According to F-statistics and assignment methods there was a significant differentiation between sylvatic and peridomestic bugs and gene flow was low and asymmetric, with more bugs moving from the peridomicile to the sylvatic environment. These results support the hypothesis of the Chaco region as the area of origin of T. infestans, and a limited role of sylvatic melanic T. infestans in peridomestic infestation in the Argentinean Chaco. © 2011 Elsevier B.V. |
| author |
Piccinali, Romina Valeria Marcet, Paula Lorena Ceballos, Leonardo Adrián Gurtler, Ricardo Esteban |
| author_facet |
Piccinali, Romina Valeria Marcet, Paula Lorena Ceballos, Leonardo Adrián Gurtler, Ricardo Esteban |
| author_sort |
Piccinali, Romina Valeria |
| title |
Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco |
| title_short |
Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco |
| title_full |
Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco |
| title_fullStr |
Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco |
| title_full_unstemmed |
Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco |
| title_sort |
genetic variability, phylogenetic relationships and gene flow in triatoma infestans dark morphs from the argentinean chaco |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v11_n5_p895_Piccinali http://hdl.handle.net/20.500.12110/paper_15671348_v11_n5_p895_Piccinali |
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AT piccinalirominavaleria geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT marcetpaulalorena geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT ceballosleonardoadrian geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco AT gurtlerricardoesteban geneticvariabilityphylogeneticrelationshipsandgeneflowintriatomainfestansdarkmorphsfromtheargentineanchaco |
| _version_ |
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