Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveli...
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2007
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1567133X_v7_n3_p339_Rojas http://hdl.handle.net/20.500.12110/paper_1567133X_v7_n3_p339_Rojas |
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paper:paper_1567133X_v7_n3_p339_Rojas |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 hypoxia inducible factor 1alpha zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling gene expression regulation genetics in situ hybridization metabolism molecular cloning phylogeny prenatal development zebra fish Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Zebrafish Proteins |
| spellingShingle |
Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 hypoxia inducible factor 1alpha zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling gene expression regulation genetics in situ hybridization metabolism molecular cloning phylogeny prenatal development zebra fish Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Zebrafish Proteins Centanin, Lázaro Wappner, Pablo Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish |
| topic_facet |
Blood vessels Brain Cartilage Dorsal aorta Hypoxia-inducible factor Intersegmental vessels Neuromasts Notochord Optic tectum Ventricle epithelia Zebrafish hypoxia inducible factor 1alpha hypoxia inducible factor 2alpha messenger RNA oxygen basic helix loop helix transcription factor endothelial PAS domain containing protein 1 endothelial PAS domain-containing protein 1 hypoxia inducible factor 1alpha zebrafish protein angiogenesis animal cell article blood vessel brain tissue controlled study developmental stage embryo embryo development expressed sequence tag gene expression gene expression regulation gene function genetic transcription heart heart development hypoxia molecular cloning molecular mechanics nervous system development nonhuman notochord nucleotide sequence oxygen tension phylogenetic tree priority journal protein domain reverse transcription polymerase chain reaction sequence analysis sequence homology species difference vertebrate zebra fish animal animal embryo antibody specificity gene expression profiling gene expression regulation genetics in situ hybridization metabolism molecular cloning phylogeny prenatal development zebra fish Danio rerio Vertebrata Animals Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit In Situ Hybridization Organ Specificity Phylogeny Zebrafish Zebrafish Proteins |
| description |
Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1α and hif-2α genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1α and hif-2α genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1α and hif-2α are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1α is switched off, while hif-2α transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1α and hif-2α is directly assessed in a vertebrate model system throughout development. © 2006 Elsevier B.V. All rights reserved. |
| author |
Centanin, Lázaro Wappner, Pablo |
| author_facet |
Centanin, Lázaro Wappner, Pablo |
| author_sort |
Centanin, Lázaro |
| title |
Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish |
| title_short |
Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish |
| title_full |
Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish |
| title_fullStr |
Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish |
| title_full_unstemmed |
Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish |
| title_sort |
cloning of hif-1α and hif-2α and mrna expression pattern during development in zebrafish |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1567133X_v7_n3_p339_Rojas http://hdl.handle.net/20.500.12110/paper_1567133X_v7_n3_p339_Rojas |
| work_keys_str_mv |
AT centaninlazaro cloningofhif1aandhif2aandmrnaexpressionpatternduringdevelopmentinzebrafish AT wappnerpablo cloningofhif1aandhif2aandmrnaexpressionpatternduringdevelopmentinzebrafish |
| bdutipo_str |
Repositorios |
| _version_ |
1764820568528388099 |