Pollen aquaporins: What are they there for?

In order to provide more insight into the function of aquaporins during pollination, we characterized NIP4;1 and NIP4;2, 2 pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 displayed high amino acid identity. RT-PCR and GUS promoter analysis showed that they have different expres...

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Detalles Bibliográficos
Publicado: 2016
Materias:
Aquaporin
pollen
pollen hydration
pollination
aquaporin
Arabidopsis protein
Arabidopsis
genetics
metabolism
physiology
pollen tube
promoter region
Aquaporins
Arabidopsis Proteins
Pollen Tube
Pollination
Promoter Regions, Genetic
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio
http://hdl.handle.net/20.500.12110/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_15592324_v11_n9_pe1217375_PerezDiGiorgio
record_format dspace
spelling paper:paper_15592324_v11_n9_pe1217375_PerezDiGiorgio2023-06-08T16:23:45Z Pollen aquaporins: What are they there for? Aquaporin pollen pollen hydration pollination aquaporin Arabidopsis protein Arabidopsis genetics metabolism physiology pollen tube pollination promoter region Aquaporins Arabidopsis Arabidopsis Proteins Pollen Tube Pollination Promoter Regions, Genetic In order to provide more insight into the function of aquaporins during pollination, we characterized NIP4;1 and NIP4;2, 2 pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 displayed high amino acid identity. RT-PCR and GUS promoter analysis showed that they have different expression patterns. NIP4;1 is expressed at low levels in mature pollen, while NIP4;2 is highly expressed only during pollen tube growth. Single T-DNA nip4;1 and nip4;2 mutants and double amiRNA nip4;1 nip4;2 knockdowns showed reduced male fertility due to deficient pollen germination and pollen tube length. Functional assays in oocytes showed that NIP4;1 and NIP4;2 transport water and nonionic solutes. Here, the participation of the different pollen aquaporins in pollen hydration and pollen tube growth is discussed. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio http://hdl.handle.net/20.500.12110/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Aquaporin
pollen
pollen hydration
pollination
aquaporin
Arabidopsis protein
Arabidopsis
genetics
metabolism
physiology
pollen tube
pollination
promoter region
Aquaporins
Arabidopsis
Arabidopsis Proteins
Pollen Tube
Pollination
Promoter Regions, Genetic
spellingShingle Aquaporin
pollen
pollen hydration
pollination
aquaporin
Arabidopsis protein
Arabidopsis
genetics
metabolism
physiology
pollen tube
pollination
promoter region
Aquaporins
Arabidopsis
Arabidopsis Proteins
Pollen Tube
Pollination
Promoter Regions, Genetic
Pollen aquaporins: What are they there for?
topic_facet Aquaporin
pollen
pollen hydration
pollination
aquaporin
Arabidopsis protein
Arabidopsis
genetics
metabolism
physiology
pollen tube
pollination
promoter region
Aquaporins
Arabidopsis
Arabidopsis Proteins
Pollen Tube
Pollination
Promoter Regions, Genetic
description In order to provide more insight into the function of aquaporins during pollination, we characterized NIP4;1 and NIP4;2, 2 pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 displayed high amino acid identity. RT-PCR and GUS promoter analysis showed that they have different expression patterns. NIP4;1 is expressed at low levels in mature pollen, while NIP4;2 is highly expressed only during pollen tube growth. Single T-DNA nip4;1 and nip4;2 mutants and double amiRNA nip4;1 nip4;2 knockdowns showed reduced male fertility due to deficient pollen germination and pollen tube length. Functional assays in oocytes showed that NIP4;1 and NIP4;2 transport water and nonionic solutes. Here, the participation of the different pollen aquaporins in pollen hydration and pollen tube growth is discussed.
title Pollen aquaporins: What are they there for?
title_short Pollen aquaporins: What are they there for?
title_full Pollen aquaporins: What are they there for?
title_fullStr Pollen aquaporins: What are they there for?
title_full_unstemmed Pollen aquaporins: What are they there for?
title_sort pollen aquaporins: what are they there for?
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio
http://hdl.handle.net/20.500.12110/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio
_version_ 1768542957225377792

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