Bottom-up Assembly of the Phytochrome Network

Plants have developed sophisticated systems to monitor and rapidly acclimate to environmental fluctuations. Light is an essential source of environmental information throughout the plant’s life cycle. The model plant Arabidopsis thaliana possesses five phytochromes (phyA-phyE) with important roles i...

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Autor principal:	Cerdán, Pablo Diego
Publicado:	2016
Materias:	phytochrome phytochrome A phytochrome B phytochrome C phytochrome D phytochrome E unclassified drug apoprotein Arabidopsis protein PHYA protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome C, Arabidopsis Article cellular distribution controlled study environmental temperature flowering gene regulatory network genotype germination molecular interaction nonhuman photoperiodicity sensitivity analysis signal transduction temperature dependence Arabidopsis genetics growth, development and aging light metabolism plant leaf seedling temperature Apoproteins Arabidopsis Proteins Genotype Germination Light Phytochrome Phytochrome A Phytochrome B Plant Leaves Seedlings Signal Transduction Temperature
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15537390_v12_n11_p_SanchezLamas http://hdl.handle.net/20.500.12110/paper_15537390_v12_n11_p_SanchezLamas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15537390_v12_n11_p_SanchezLamas
record_format	dspace
spelling	paper:paper_15537390_v12_n11_p_SanchezLamas2023-06-08T16:23:11Z Bottom-up Assembly of the Phytochrome Network Cerdán, Pablo Diego phytochrome phytochrome A phytochrome B phytochrome C phytochrome D phytochrome E unclassified drug apoprotein Arabidopsis protein PHYA protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome C, Arabidopsis Article cellular distribution controlled study environmental temperature flowering gene regulatory network genotype germination molecular interaction nonhuman photoperiodicity sensitivity analysis signal transduction temperature dependence Arabidopsis genetics growth, development and aging light metabolism plant leaf seedling temperature Apoproteins Arabidopsis Arabidopsis Proteins Genotype Germination Light Phytochrome Phytochrome A Phytochrome B Plant Leaves Seedlings Signal Transduction Temperature Plants have developed sophisticated systems to monitor and rapidly acclimate to environmental fluctuations. Light is an essential source of environmental information throughout the plant’s life cycle. The model plant Arabidopsis thaliana possesses five phytochromes (phyA-phyE) with important roles in germination, seedling establishment, shade avoidance, and flowering. However, our understanding of the phytochrome signaling network is incomplete, and little is known about the individual roles of phytochromes and how they function cooperatively to mediate light responses. Here, we used a bottom-up approach to study the phytochrome network. We added each of the five phytochromes to a phytochrome-less background to study their individual roles and then added the phytochromes by pairs to study their interactions. By analyzing the 16 resulting genotypes, we revealed unique roles for each phytochrome and identified novel phytochrome interactions that regulate germination and the onset of flowering. Furthermore, we found that ambient temperature has both phytochrome-dependent and -independent effects, suggesting that multiple pathways integrate temperature and light signaling. Surprisingly, none of the phytochromes alone conferred a photoperiodic response. Although phyE and phyB were the strongest repressors of flowering, both phyB and phyC were needed to confer a flowering response to photoperiod. Thus, a specific combination of phytochromes is required to detect changes in photoperiod, whereas single phytochromes are sufficient to respond to light quality, indicating how phytochromes signal different light cues. © 2016 Sánchez-Lamas et al. Fil:Cerdán, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15537390_v12_n11_p_SanchezLamas http://hdl.handle.net/20.500.12110/paper_15537390_v12_n11_p_SanchezLamas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	phytochrome phytochrome A phytochrome B phytochrome C phytochrome D phytochrome E unclassified drug apoprotein Arabidopsis protein PHYA protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome C, Arabidopsis Article cellular distribution controlled study environmental temperature flowering gene regulatory network genotype germination molecular interaction nonhuman photoperiodicity sensitivity analysis signal transduction temperature dependence Arabidopsis genetics growth, development and aging light metabolism plant leaf seedling temperature Apoproteins Arabidopsis Arabidopsis Proteins Genotype Germination Light Phytochrome Phytochrome A Phytochrome B Plant Leaves Seedlings Signal Transduction Temperature
spellingShingle	phytochrome phytochrome A phytochrome B phytochrome C phytochrome D phytochrome E unclassified drug apoprotein Arabidopsis protein PHYA protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome C, Arabidopsis Article cellular distribution controlled study environmental temperature flowering gene regulatory network genotype germination molecular interaction nonhuman photoperiodicity sensitivity analysis signal transduction temperature dependence Arabidopsis genetics growth, development and aging light metabolism plant leaf seedling temperature Apoproteins Arabidopsis Arabidopsis Proteins Genotype Germination Light Phytochrome Phytochrome A Phytochrome B Plant Leaves Seedlings Signal Transduction Temperature Cerdán, Pablo Diego Bottom-up Assembly of the Phytochrome Network
topic_facet	phytochrome phytochrome A phytochrome B phytochrome C phytochrome D phytochrome E unclassified drug apoprotein Arabidopsis protein PHYA protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome C, Arabidopsis Article cellular distribution controlled study environmental temperature flowering gene regulatory network genotype germination molecular interaction nonhuman photoperiodicity sensitivity analysis signal transduction temperature dependence Arabidopsis genetics growth, development and aging light metabolism plant leaf seedling temperature Apoproteins Arabidopsis Arabidopsis Proteins Genotype Germination Light Phytochrome Phytochrome A Phytochrome B Plant Leaves Seedlings Signal Transduction Temperature
description	Plants have developed sophisticated systems to monitor and rapidly acclimate to environmental fluctuations. Light is an essential source of environmental information throughout the plant’s life cycle. The model plant Arabidopsis thaliana possesses five phytochromes (phyA-phyE) with important roles in germination, seedling establishment, shade avoidance, and flowering. However, our understanding of the phytochrome signaling network is incomplete, and little is known about the individual roles of phytochromes and how they function cooperatively to mediate light responses. Here, we used a bottom-up approach to study the phytochrome network. We added each of the five phytochromes to a phytochrome-less background to study their individual roles and then added the phytochromes by pairs to study their interactions. By analyzing the 16 resulting genotypes, we revealed unique roles for each phytochrome and identified novel phytochrome interactions that regulate germination and the onset of flowering. Furthermore, we found that ambient temperature has both phytochrome-dependent and -independent effects, suggesting that multiple pathways integrate temperature and light signaling. Surprisingly, none of the phytochromes alone conferred a photoperiodic response. Although phyE and phyB were the strongest repressors of flowering, both phyB and phyC were needed to confer a flowering response to photoperiod. Thus, a specific combination of phytochromes is required to detect changes in photoperiod, whereas single phytochromes are sufficient to respond to light quality, indicating how phytochromes signal different light cues. © 2016 Sánchez-Lamas et al.
author	Cerdán, Pablo Diego
author_facet	Cerdán, Pablo Diego
author_sort	Cerdán, Pablo Diego
title	Bottom-up Assembly of the Phytochrome Network
title_short	Bottom-up Assembly of the Phytochrome Network
title_full	Bottom-up Assembly of the Phytochrome Network
title_fullStr	Bottom-up Assembly of the Phytochrome Network
title_full_unstemmed	Bottom-up Assembly of the Phytochrome Network
title_sort	bottom-up assembly of the phytochrome network
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15537390_v12_n11_p_SanchezLamas http://hdl.handle.net/20.500.12110/paper_15537390_v12_n11_p_SanchezLamas
work_keys_str_mv	AT cerdanpablodiego bottomupassemblyofthephytochromenetwork
_version_	1768545162223419392

Bottom-up Assembly of the Phytochrome Network

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