Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura)
This paper analyzes, in the toad Bufo arenarum, the effect on spermiation and androgen secretion of two human recombinant gonadotropins, human recombinant LH (hrLH) and human recombinant FSH (hrFSH) as well as the well-known spermiation-inducing hormone, human chorionic gonadotropin (hCG). For this...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15488969_v305A_n1_p96_Pozzi http://hdl.handle.net/20.500.12110/paper_15488969_v305A_n1_p96_Pozzi |
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paper:paper_15488969_v305A_n1_p96_Pozzi2023-06-08T16:21:21Z Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura) Pozzi, Andrea Gabriela Rosemblit, Cinthia Ceballos, Nora Raquel Amphibia Anura Bufo arenarum chorionic gonadotropin follitropin luteinizing hormone testosterone animal article biosynthesis comparative study dose response drug effect human male physiology secretion spermatogenesis spermatozoon testis toad Animals Bufo arenarum Chorionic Gonadotropin Dose-Response Relationship, Drug Follicle Stimulating Hormone Humans Luteinizing Hormone Male Spermatogenesis Spermatozoa Testis Testosterone This paper analyzes, in the toad Bufo arenarum, the effect on spermiation and androgen secretion of two human recombinant gonadotropins, human recombinant LH (hrLH) and human recombinant FSH (hrFSH) as well as the well-known spermiation-inducing hormone, human chorionic gonadotropin (hCG). For this purpose, testes were incubated with different concentrations of hrLH (0.01-2.5 μg/ml) and hrFSH (0.05-5 μg/ml), and results were compared with those obtained with 2.5 μg/ml hCG. Spermiation was most efficiently stimulated by hrFSH, which elicited a higher response than either hrLH or hCG. Both hrFSH and hrLH produced a bell-shaped dose-response curve, with a 50% inhibition on spermiation at a concentration twice higher than that necessary to get the highest response. However, none of the gonadotropins yielded a biphasic response on androgen secretion, hrLH producing the highest response at a concentration that evoked a 70% inhibition in the spermiation test. Regarding steroidogenesis, hrLH and hrFSH were more active than hCG. Taken together, the results described in this paper suggest that, in B. arenarum, spermiation and androgen secretion are mediated by different receptors. After comparing the effects of recombinant hormones, we conclude that hrFSH has a greater effect on spermiation than hCG or hrLH. © 2005 Wiley-Liss, Inc. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosemblit, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15488969_v305A_n1_p96_Pozzi http://hdl.handle.net/20.500.12110/paper_15488969_v305A_n1_p96_Pozzi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amphibia Anura Bufo arenarum chorionic gonadotropin follitropin luteinizing hormone testosterone animal article biosynthesis comparative study dose response drug effect human male physiology secretion spermatogenesis spermatozoon testis toad Animals Bufo arenarum Chorionic Gonadotropin Dose-Response Relationship, Drug Follicle Stimulating Hormone Humans Luteinizing Hormone Male Spermatogenesis Spermatozoa Testis Testosterone |
| spellingShingle |
Amphibia Anura Bufo arenarum chorionic gonadotropin follitropin luteinizing hormone testosterone animal article biosynthesis comparative study dose response drug effect human male physiology secretion spermatogenesis spermatozoon testis toad Animals Bufo arenarum Chorionic Gonadotropin Dose-Response Relationship, Drug Follicle Stimulating Hormone Humans Luteinizing Hormone Male Spermatogenesis Spermatozoa Testis Testosterone Pozzi, Andrea Gabriela Rosemblit, Cinthia Ceballos, Nora Raquel Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura) |
| topic_facet |
Amphibia Anura Bufo arenarum chorionic gonadotropin follitropin luteinizing hormone testosterone animal article biosynthesis comparative study dose response drug effect human male physiology secretion spermatogenesis spermatozoon testis toad Animals Bufo arenarum Chorionic Gonadotropin Dose-Response Relationship, Drug Follicle Stimulating Hormone Humans Luteinizing Hormone Male Spermatogenesis Spermatozoa Testis Testosterone |
| description |
This paper analyzes, in the toad Bufo arenarum, the effect on spermiation and androgen secretion of two human recombinant gonadotropins, human recombinant LH (hrLH) and human recombinant FSH (hrFSH) as well as the well-known spermiation-inducing hormone, human chorionic gonadotropin (hCG). For this purpose, testes were incubated with different concentrations of hrLH (0.01-2.5 μg/ml) and hrFSH (0.05-5 μg/ml), and results were compared with those obtained with 2.5 μg/ml hCG. Spermiation was most efficiently stimulated by hrFSH, which elicited a higher response than either hrLH or hCG. Both hrFSH and hrLH produced a bell-shaped dose-response curve, with a 50% inhibition on spermiation at a concentration twice higher than that necessary to get the highest response. However, none of the gonadotropins yielded a biphasic response on androgen secretion, hrLH producing the highest response at a concentration that evoked a 70% inhibition in the spermiation test. Regarding steroidogenesis, hrLH and hrFSH were more active than hCG. Taken together, the results described in this paper suggest that, in B. arenarum, spermiation and androgen secretion are mediated by different receptors. After comparing the effects of recombinant hormones, we conclude that hrFSH has a greater effect on spermiation than hCG or hrLH. © 2005 Wiley-Liss, Inc. |
| author |
Pozzi, Andrea Gabriela Rosemblit, Cinthia Ceballos, Nora Raquel |
| author_facet |
Pozzi, Andrea Gabriela Rosemblit, Cinthia Ceballos, Nora Raquel |
| author_sort |
Pozzi, Andrea Gabriela |
| title |
Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura) |
| title_short |
Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura) |
| title_full |
Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura) |
| title_fullStr |
Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura) |
| title_full_unstemmed |
Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura) |
| title_sort |
effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad bufo arenarum (amphibia, anura) |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15488969_v305A_n1_p96_Pozzi http://hdl.handle.net/20.500.12110/paper_15488969_v305A_n1_p96_Pozzi |
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| _version_ |
1768544244128022528 |