Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients
Previously, we reported a deficient cloning capacity of the bone marrow (BM) mesenchymal stem cells to give colony-forming unit fibroblast (CFU-F) and an inefficient confluence capacity of BM stromal cells in advanced untreated lung cancer patients (LCP) and breast cancer patients (BCP). Moreover, a...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v14_n5_p587_Hofer http://hdl.handle.net/20.500.12110/paper_15473287_v14_n5_p587_Hofer |
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paper:paper_15473287_v14_n5_p587_Hofer2023-06-08T16:21:16Z Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients cytokeratin 20 cytokeratin 7 epidermal growth factor receptor epithelial membrane antigen fibroblast growth factor receptor interleukin 1 receptor monoclonal antibody Myc protein oncoprotein platelet derived growth factor receptor polyclonal antibody protein c fos transforming growth factor beta receptor article bone marrow cell bone marrow metastasis breast carcinoma cancer infiltration cell proliferation colony forming unit F controlled study fibroblast human human cell human tissue immunocytochemistry lung non small cell cancer mesenchymal stem cell priority journal prognosis protein expression stroma cell Animals Bone Marrow Cells Breast Neoplasms Cells, Cultured Culture Media, Conditioned Female Fibroblasts Humans Lung Neoplasms Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-myc Receptor, Epidermal Growth Factor Receptors, Fibroblast Growth Factor Receptors, Interleukin-1 Receptors, Platelet-Derived Growth Factor Receptors, Transforming Growth Factor beta Stromal Cells Tumor Stem Cell Assay Previously, we reported a deficient cloning capacity of the bone marrow (BM) mesenchymal stem cells to give colony-forming unit fibroblast (CFU-F) and an inefficient confluence capacity of BM stromal cells in advanced untreated lung cancer patients (LCP) and breast cancer patients (BCP). Moreover, a decreased level of bFGF at day 7 in the conditioned media from BM CFU-F cultures was found in both cancer groups when compared to the normal range. The current study was specially undertaken, to evaluate the percentage of subconfluent fibroblasts expressing receptors (R) of interleukin-1 (IL-1), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor (TGF-β), epidermal growth factor (EGF), and the proteins c-Fos and c-Myc in BM primary cultures from untreated LCP and BCP. An immunocytochemical study on subconfluent BM fibroblast cultures from 13 healthy patients, 16 LCP, and 8 BCP was performed, using as primary antibodies, anti-type I of IL-1 R (IL-1R-1), anti-α, β chains of PDGF R (PDGFR-α, PDGFR-β), anti-type I of FGF R (FGFR-I), anti-type I, II, and III of TGF-β R (TGF-βR-I, TGF-βR-II, and TGF-βR-III), anti-EGF R, anti-c-Fos, and anti-c-Myc. A diminished percentage of subconfluent fibroblasts expressing PDGFR-α, TGFβR-I, II, III, EGFR, and FGFR-I was found in LCP and BCP compared to healthy patients. A diminished percentage of subconfluent fibroblasts expressing c-Fos and c-Myc was found in patients when compared to healthy patients. The alterations we describe could help to explain the deficiency regarding the proliferative and confluence capacity of BM stroma cells in cancer patients. © Mary Ann Liebert. Inc. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v14_n5_p587_Hofer http://hdl.handle.net/20.500.12110/paper_15473287_v14_n5_p587_Hofer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cytokeratin 20 cytokeratin 7 epidermal growth factor receptor epithelial membrane antigen fibroblast growth factor receptor interleukin 1 receptor monoclonal antibody Myc protein oncoprotein platelet derived growth factor receptor polyclonal antibody protein c fos transforming growth factor beta receptor article bone marrow cell bone marrow metastasis breast carcinoma cancer infiltration cell proliferation colony forming unit F controlled study fibroblast human human cell human tissue immunocytochemistry lung non small cell cancer mesenchymal stem cell priority journal prognosis protein expression stroma cell Animals Bone Marrow Cells Breast Neoplasms Cells, Cultured Culture Media, Conditioned Female Fibroblasts Humans Lung Neoplasms Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-myc Receptor, Epidermal Growth Factor Receptors, Fibroblast Growth Factor Receptors, Interleukin-1 Receptors, Platelet-Derived Growth Factor Receptors, Transforming Growth Factor beta Stromal Cells Tumor Stem Cell Assay |
| spellingShingle |
cytokeratin 20 cytokeratin 7 epidermal growth factor receptor epithelial membrane antigen fibroblast growth factor receptor interleukin 1 receptor monoclonal antibody Myc protein oncoprotein platelet derived growth factor receptor polyclonal antibody protein c fos transforming growth factor beta receptor article bone marrow cell bone marrow metastasis breast carcinoma cancer infiltration cell proliferation colony forming unit F controlled study fibroblast human human cell human tissue immunocytochemistry lung non small cell cancer mesenchymal stem cell priority journal prognosis protein expression stroma cell Animals Bone Marrow Cells Breast Neoplasms Cells, Cultured Culture Media, Conditioned Female Fibroblasts Humans Lung Neoplasms Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-myc Receptor, Epidermal Growth Factor Receptors, Fibroblast Growth Factor Receptors, Interleukin-1 Receptors, Platelet-Derived Growth Factor Receptors, Transforming Growth Factor beta Stromal Cells Tumor Stem Cell Assay Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients |
| topic_facet |
cytokeratin 20 cytokeratin 7 epidermal growth factor receptor epithelial membrane antigen fibroblast growth factor receptor interleukin 1 receptor monoclonal antibody Myc protein oncoprotein platelet derived growth factor receptor polyclonal antibody protein c fos transforming growth factor beta receptor article bone marrow cell bone marrow metastasis breast carcinoma cancer infiltration cell proliferation colony forming unit F controlled study fibroblast human human cell human tissue immunocytochemistry lung non small cell cancer mesenchymal stem cell priority journal prognosis protein expression stroma cell Animals Bone Marrow Cells Breast Neoplasms Cells, Cultured Culture Media, Conditioned Female Fibroblasts Humans Lung Neoplasms Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-myc Receptor, Epidermal Growth Factor Receptors, Fibroblast Growth Factor Receptors, Interleukin-1 Receptors, Platelet-Derived Growth Factor Receptors, Transforming Growth Factor beta Stromal Cells Tumor Stem Cell Assay |
| description |
Previously, we reported a deficient cloning capacity of the bone marrow (BM) mesenchymal stem cells to give colony-forming unit fibroblast (CFU-F) and an inefficient confluence capacity of BM stromal cells in advanced untreated lung cancer patients (LCP) and breast cancer patients (BCP). Moreover, a decreased level of bFGF at day 7 in the conditioned media from BM CFU-F cultures was found in both cancer groups when compared to the normal range. The current study was specially undertaken, to evaluate the percentage of subconfluent fibroblasts expressing receptors (R) of interleukin-1 (IL-1), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor (TGF-β), epidermal growth factor (EGF), and the proteins c-Fos and c-Myc in BM primary cultures from untreated LCP and BCP. An immunocytochemical study on subconfluent BM fibroblast cultures from 13 healthy patients, 16 LCP, and 8 BCP was performed, using as primary antibodies, anti-type I of IL-1 R (IL-1R-1), anti-α, β chains of PDGF R (PDGFR-α, PDGFR-β), anti-type I of FGF R (FGFR-I), anti-type I, II, and III of TGF-β R (TGF-βR-I, TGF-βR-II, and TGF-βR-III), anti-EGF R, anti-c-Fos, and anti-c-Myc. A diminished percentage of subconfluent fibroblasts expressing PDGFR-α, TGFβR-I, II, III, EGFR, and FGFR-I was found in LCP and BCP compared to healthy patients. A diminished percentage of subconfluent fibroblasts expressing c-Fos and c-Myc was found in patients when compared to healthy patients. The alterations we describe could help to explain the deficiency regarding the proliferative and confluence capacity of BM stroma cells in cancer patients. © Mary Ann Liebert. Inc. |
| title |
Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients |
| title_short |
Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients |
| title_full |
Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients |
| title_fullStr |
Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients |
| title_full_unstemmed |
Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients |
| title_sort |
alteration on the expression of il-1, pdgf, tgf-β, egf, and fgf receptors and c-fos and c-myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v14_n5_p587_Hofer http://hdl.handle.net/20.500.12110/paper_15473287_v14_n5_p587_Hofer |
| _version_ |
1768543721857482752 |