Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption

The aim was to study the control females (CF)-1 mouse embryo differentiation, growth, morphology on embryonic E- and N-cadherin expression at midgestation after periconceptional moderate alcohol ingestion. Adult female mice were exposed to 10% ethanol in drinking water for 17 days previous to and up...

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Autores principales:	Coll, Tamara Anahi, Perez Tito, Leticia Gabriela, Cebral, Elisa
Publicado:	2011
Materias:	Alcohol Cadherins CF-1 mouse Differentiation Growth Morphology Organogenesis alcohol cell adhesion molecule nerve cell adhesion molecule uvomorulin alcohol consumption animal experiment animal model article body size conception controlled study developmental disorder embryo embryo development embryo growth female growth retardation immunohistochemistry mesenchyme mouse mouse strain neural tube defect neuroepithelium nonhuman organogenesis priority journal protein expression scanning electron microscopy somite statistical significance upregulation Alcohol Drinking Animals Blotting, Western Drinking Water Embryo, Mammalian Female Immunoenzyme Techniques Maternal Exposure Mice Neural Tube Defects Pregnancy Prenatal Exposure Delayed Effects
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15429733_v92_n6_p560_Coll http://hdl.handle.net/20.500.12110/paper_15429733_v92_n6_p560_Coll
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_15429733_v92_n6_p560_Coll2023-06-08T16:21:08Z Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption Coll, Tamara Anahi Perez Tito, Leticia Gabriela Cebral, Elisa Alcohol Cadherins CF-1 mouse Differentiation Growth Morphology Organogenesis alcohol cell adhesion molecule nerve cell adhesion molecule uvomorulin alcohol consumption animal experiment animal model article body size conception controlled study developmental disorder embryo embryo development embryo growth female growth retardation immunohistochemistry mesenchyme mouse mouse strain neural tube defect neuroepithelium nonhuman organogenesis priority journal protein expression scanning electron microscopy somite statistical significance upregulation Alcohol Drinking Animals Blotting, Western Cadherins Drinking Water Embryo, Mammalian Female Immunoenzyme Techniques Maternal Exposure Mice Neural Tube Defects Organogenesis Pregnancy Prenatal Exposure Delayed Effects The aim was to study the control females (CF)-1 mouse embryo differentiation, growth, morphology on embryonic E- and N-cadherin expression at midgestation after periconceptional moderate alcohol ingestion. Adult female mice were exposed to 10% ethanol in drinking water for 17 days previous to and up to day 10 of gestation (ethanol-exposed females, EF) and were compared with nonexposed CF. EF presented reduced quantities of E10 to E10.5 embryos, greater percentage of embryos at stages less than E7.5, reduced implantation site numbers/female, and increased resorptions compared with CF. EF-embryo growth was significantly affected as evidenced by reduced cephalic and body sizes of E10 and E10.5 embryos (scanning electron microscopy) and decreased protein content of E10.5 embryos vs. CF embryos. A significantly higher percentage of EF-E10-10.5 embryos presented abnormal neural tube (NT) closure vs. the percentage of CF. E10 embryos from EF presented elevated tissue disorganization, pyknosis and nuclear condensation in somites, mesenchymal and neuroepithelial tissue. Immunohistochemical E- and N-cadherin distribution patterns were similar in organic structures of E10 embryos between groups. However, western blot revealed that E- and N-cadherin expression levels were significantly increased in EF-derived embryos vs. controls. Perigestational ethanol consumption by CF-1 mice induced significant damage in the organogenic embryogenesis by producing delayed differentiation, growth deficiencies, and increasing the frequency of NT defects. Ethanol exposure may disrupt cell-cell adhesion leading to upregulation of E- and N-cadherin expression suggesting that deregulation of cell adhesion molecules could be involved in the disruption of embryo development at organogenesis in CF-1 mouse. © 2011 Wiley Periodicals, Inc. Fil:Coll, T.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tito, L.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15429733_v92_n6_p560_Coll http://hdl.handle.net/20.500.12110/paper_15429733_v92_n6_p560_Coll
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alcohol Cadherins CF-1 mouse Differentiation Growth Morphology Organogenesis alcohol cell adhesion molecule nerve cell adhesion molecule uvomorulin alcohol consumption animal experiment animal model article body size conception controlled study developmental disorder embryo embryo development embryo growth female growth retardation immunohistochemistry mesenchyme mouse mouse strain neural tube defect neuroepithelium nonhuman organogenesis priority journal protein expression scanning electron microscopy somite statistical significance upregulation Alcohol Drinking Animals Blotting, Western Cadherins Drinking Water Embryo, Mammalian Female Immunoenzyme Techniques Maternal Exposure Mice Neural Tube Defects Organogenesis Pregnancy Prenatal Exposure Delayed Effects
spellingShingle	Alcohol Cadherins CF-1 mouse Differentiation Growth Morphology Organogenesis alcohol cell adhesion molecule nerve cell adhesion molecule uvomorulin alcohol consumption animal experiment animal model article body size conception controlled study developmental disorder embryo embryo development embryo growth female growth retardation immunohistochemistry mesenchyme mouse mouse strain neural tube defect neuroepithelium nonhuman organogenesis priority journal protein expression scanning electron microscopy somite statistical significance upregulation Alcohol Drinking Animals Blotting, Western Cadherins Drinking Water Embryo, Mammalian Female Immunoenzyme Techniques Maternal Exposure Mice Neural Tube Defects Organogenesis Pregnancy Prenatal Exposure Delayed Effects Coll, Tamara Anahi Perez Tito, Leticia Gabriela Cebral, Elisa Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption
topic_facet	Alcohol Cadherins CF-1 mouse Differentiation Growth Morphology Organogenesis alcohol cell adhesion molecule nerve cell adhesion molecule uvomorulin alcohol consumption animal experiment animal model article body size conception controlled study developmental disorder embryo embryo development embryo growth female growth retardation immunohistochemistry mesenchyme mouse mouse strain neural tube defect neuroepithelium nonhuman organogenesis priority journal protein expression scanning electron microscopy somite statistical significance upregulation Alcohol Drinking Animals Blotting, Western Cadherins Drinking Water Embryo, Mammalian Female Immunoenzyme Techniques Maternal Exposure Mice Neural Tube Defects Organogenesis Pregnancy Prenatal Exposure Delayed Effects
description	The aim was to study the control females (CF)-1 mouse embryo differentiation, growth, morphology on embryonic E- and N-cadherin expression at midgestation after periconceptional moderate alcohol ingestion. Adult female mice were exposed to 10% ethanol in drinking water for 17 days previous to and up to day 10 of gestation (ethanol-exposed females, EF) and were compared with nonexposed CF. EF presented reduced quantities of E10 to E10.5 embryos, greater percentage of embryos at stages less than E7.5, reduced implantation site numbers/female, and increased resorptions compared with CF. EF-embryo growth was significantly affected as evidenced by reduced cephalic and body sizes of E10 and E10.5 embryos (scanning electron microscopy) and decreased protein content of E10.5 embryos vs. CF embryos. A significantly higher percentage of EF-E10-10.5 embryos presented abnormal neural tube (NT) closure vs. the percentage of CF. E10 embryos from EF presented elevated tissue disorganization, pyknosis and nuclear condensation in somites, mesenchymal and neuroepithelial tissue. Immunohistochemical E- and N-cadherin distribution patterns were similar in organic structures of E10 embryos between groups. However, western blot revealed that E- and N-cadherin expression levels were significantly increased in EF-derived embryos vs. controls. Perigestational ethanol consumption by CF-1 mice induced significant damage in the organogenic embryogenesis by producing delayed differentiation, growth deficiencies, and increasing the frequency of NT defects. Ethanol exposure may disrupt cell-cell adhesion leading to upregulation of E- and N-cadherin expression suggesting that deregulation of cell adhesion molecules could be involved in the disruption of embryo development at organogenesis in CF-1 mouse. © 2011 Wiley Periodicals, Inc.
author	Coll, Tamara Anahi Perez Tito, Leticia Gabriela Cebral, Elisa
author_facet	Coll, Tamara Anahi Perez Tito, Leticia Gabriela Cebral, Elisa
author_sort	Coll, Tamara Anahi
title	Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption
title_short	Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption
title_full	Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption
title_fullStr	Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption
title_full_unstemmed	Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption
title_sort	embryo developmental disruption during organogenesis produced by cf-1 murine periconceptional alcohol consumption
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15429733_v92_n6_p560_Coll http://hdl.handle.net/20.500.12110/paper_15429733_v92_n6_p560_Coll
work_keys_str_mv	AT colltamaraanahi embryodevelopmentaldisruptionduringorganogenesisproducedbycf1murinepericonceptionalalcoholconsumption AT pereztitoleticiagabriela embryodevelopmentaldisruptionduringorganogenesisproducedbycf1murinepericonceptionalalcoholconsumption AT cebralelisa embryodevelopmentaldisruptionduringorganogenesisproducedbycf1murinepericonceptionalalcoholconsumption
_version_	1768545161666625536

Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption

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