Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman http://hdl.handle.net/20.500.12110/paper_15421406_v430_n_p257_JaresErijman |
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paper:paper_15421406_v430_n_p257_JaresErijman2023-06-08T16:21:07Z Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) Jares, Elizabeth Andrea Giordano, Luciana Spagnuolo, Carla Cecilia Energy transfer Fluorescence Imaging techniques Nanostructured materials Photochromism Resonance Conjugated streptavidin Diheteroarylethene derivatives Photochromic fluorescence resonance energy transfer (pcFRET) Semiconductor nanocrystals Semiconductor quantum dots The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546 nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging. Fil:Jares-Erijman, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giordano, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spagnuolo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman http://hdl.handle.net/20.500.12110/paper_15421406_v430_n_p257_JaresErijman |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Energy transfer Fluorescence Imaging techniques Nanostructured materials Photochromism Resonance Conjugated streptavidin Diheteroarylethene derivatives Photochromic fluorescence resonance energy transfer (pcFRET) Semiconductor nanocrystals Semiconductor quantum dots |
spellingShingle |
Energy transfer Fluorescence Imaging techniques Nanostructured materials Photochromism Resonance Conjugated streptavidin Diheteroarylethene derivatives Photochromic fluorescence resonance energy transfer (pcFRET) Semiconductor nanocrystals Semiconductor quantum dots Jares, Elizabeth Andrea Giordano, Luciana Spagnuolo, Carla Cecilia Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) |
topic_facet |
Energy transfer Fluorescence Imaging techniques Nanostructured materials Photochromism Resonance Conjugated streptavidin Diheteroarylethene derivatives Photochromic fluorescence resonance energy transfer (pcFRET) Semiconductor nanocrystals Semiconductor quantum dots |
description |
The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546 nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging. |
author |
Jares, Elizabeth Andrea Giordano, Luciana Spagnuolo, Carla Cecilia |
author_facet |
Jares, Elizabeth Andrea Giordano, Luciana Spagnuolo, Carla Cecilia |
author_sort |
Jares, Elizabeth Andrea |
title |
Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) |
title_short |
Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) |
title_full |
Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) |
title_fullStr |
Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) |
title_full_unstemmed |
Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) |
title_sort |
imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcfret) |
publishDate |
2005 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman http://hdl.handle.net/20.500.12110/paper_15421406_v430_n_p257_JaresErijman |
work_keys_str_mv |
AT jareselizabethandrea imagingquantumdotsswitchedonandoffbyphotochromicfluorescenceresonanceenergytransferpcfret AT giordanoluciana imagingquantumdotsswitchedonandoffbyphotochromicfluorescenceresonanceenergytransferpcfret AT spagnuolocarlacecilia imagingquantumdotsswitchedonandoffbyphotochromicfluorescenceresonanceenergytransferpcfret |
_version_ |
1768546508695666688 |