Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)

The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing...

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Autores principales: Jares, Elizabeth Andrea, Giordano, Luciana, Spagnuolo, Carla Cecilia
Publicado: 2005
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman
http://hdl.handle.net/20.500.12110/paper_15421406_v430_n_p257_JaresErijman
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id paper:paper_15421406_v430_n_p257_JaresErijman
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spelling paper:paper_15421406_v430_n_p257_JaresErijman2023-06-08T16:21:07Z Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) Jares, Elizabeth Andrea Giordano, Luciana Spagnuolo, Carla Cecilia Energy transfer Fluorescence Imaging techniques Nanostructured materials Photochromism Resonance Conjugated streptavidin Diheteroarylethene derivatives Photochromic fluorescence resonance energy transfer (pcFRET) Semiconductor nanocrystals Semiconductor quantum dots The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546 nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging. Fil:Jares-Erijman, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giordano, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spagnuolo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman http://hdl.handle.net/20.500.12110/paper_15421406_v430_n_p257_JaresErijman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Energy transfer
Fluorescence
Imaging techniques
Nanostructured materials
Photochromism
Resonance
Conjugated streptavidin
Diheteroarylethene derivatives
Photochromic fluorescence resonance energy transfer (pcFRET)
Semiconductor nanocrystals
Semiconductor quantum dots
spellingShingle Energy transfer
Fluorescence
Imaging techniques
Nanostructured materials
Photochromism
Resonance
Conjugated streptavidin
Diheteroarylethene derivatives
Photochromic fluorescence resonance energy transfer (pcFRET)
Semiconductor nanocrystals
Semiconductor quantum dots
Jares, Elizabeth Andrea
Giordano, Luciana
Spagnuolo, Carla Cecilia
Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
topic_facet Energy transfer
Fluorescence
Imaging techniques
Nanostructured materials
Photochromism
Resonance
Conjugated streptavidin
Diheteroarylethene derivatives
Photochromic fluorescence resonance energy transfer (pcFRET)
Semiconductor nanocrystals
Semiconductor quantum dots
description The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546 nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging.
author Jares, Elizabeth Andrea
Giordano, Luciana
Spagnuolo, Carla Cecilia
author_facet Jares, Elizabeth Andrea
Giordano, Luciana
Spagnuolo, Carla Cecilia
author_sort Jares, Elizabeth Andrea
title Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
title_short Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
title_full Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
title_fullStr Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
title_full_unstemmed Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
title_sort imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcfret)
publishDate 2005
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman
http://hdl.handle.net/20.500.12110/paper_15421406_v430_n_p257_JaresErijman
work_keys_str_mv AT jareselizabethandrea imagingquantumdotsswitchedonandoffbyphotochromicfluorescenceresonanceenergytransferpcfret
AT giordanoluciana imagingquantumdotsswitchedonandoffbyphotochromicfluorescenceresonanceenergytransferpcfret
AT spagnuolocarlacecilia imagingquantumdotsswitchedonandoffbyphotochromicfluorescenceresonanceenergytransferpcfret
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