Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine
The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15359778_v9_n8_p1262_Cardillo http://hdl.handle.net/20.500.12110/paper_15359778_v9_n8_p1262_Cardillo |
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paper:paper_15359778_v9_n8_p1262_Cardillo2023-06-08T16:20:08Z Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine Cardillo, Sabrina Beatriz 4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35/Dal81. UGA4 is subjected to a very complex regulation, and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that the Uga3 and Uga35/Dal81 transcription factors bind to the UGA4 promoter in a GABA-dependent manner and that this binding is impaired by the presence of leucine. We also found that the Leu3 transcription factor negatively regulates UGA4 transcription, although this seems to be through an indirect mechanism. © 2010, American Society for Microbiology. Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15359778_v9_n8_p1262_Cardillo http://hdl.handle.net/20.500.12110/paper_15359778_v9_n8_p1262_Cardillo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic |
| spellingShingle |
4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Cardillo, Sabrina Beatriz Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine |
| topic_facet |
4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic |
| description |
The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35/Dal81. UGA4 is subjected to a very complex regulation, and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that the Uga3 and Uga35/Dal81 transcription factors bind to the UGA4 promoter in a GABA-dependent manner and that this binding is impaired by the presence of leucine. We also found that the Leu3 transcription factor negatively regulates UGA4 transcription, although this seems to be through an indirect mechanism. © 2010, American Society for Microbiology. |
| author |
Cardillo, Sabrina Beatriz |
| author_facet |
Cardillo, Sabrina Beatriz |
| author_sort |
Cardillo, Sabrina Beatriz |
| title |
Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine |
| title_short |
Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine |
| title_full |
Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine |
| title_fullStr |
Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine |
| title_full_unstemmed |
Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine |
| title_sort |
uga3 and uga35/dal81 transcription factors regulate uga4 transcription in response to γ-aminobutyric acid and leucine |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15359778_v9_n8_p1262_Cardillo http://hdl.handle.net/20.500.12110/paper_15359778_v9_n8_p1262_Cardillo |
| work_keys_str_mv |
AT cardillosabrinabeatriz uga3anduga35dal81transcriptionfactorsregulateuga4transcriptioninresponsetogaminobutyricacidandleucine |
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1768544838757646336 |