Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on trache...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15345807_v14_n4_p547_Centanin http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin |
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paper:paper_15345807_v14_n4_p547_Centanin2023-06-08T16:20:02Z Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting Centanin, Lázaro Dekanty, Andrés Romero, Nuria Magdalena Irisarri, Maximiliano Wappner, Pablo DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved. Fil:Centanin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dekanty, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romero, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Irisarri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wappner, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15345807_v14_n4_p547_Centanin http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia |
| spellingShingle |
DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia Centanin, Lázaro Dekanty, Andrés Romero, Nuria Magdalena Irisarri, Maximiliano Wappner, Pablo Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting |
| topic_facet |
DEVBIO cell protein fibroblast growth factor receptor hypoxia inducible factor alpha ligand procollagen proline 2 oxoglutarate 4 dioxygenase protein Fatiga transcription factor transcription factor Branchless transcription factor Breathless transcription factor Sima unclassified drug angiogenesis animal cell article cell hypoxia controlled study Drosophila embryo gene overexpression nonhuman organogenesis oxygen sensing phenotype priority journal protein expression protein localization tissue oxygenation trachea wild type Animals Anoxia Body Patterning DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Fibroblast Growth Factors Gene Expression Regulation, Developmental Hypoxia-Inducible Factor 1, alpha Subunit Larva Oxygen Phenotype Procollagen-Proline Dioxygenase Protein-Tyrosine Kinases Receptors, Fibroblast Growth Factor Mammalia |
| description |
Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved. |
| author |
Centanin, Lázaro Dekanty, Andrés Romero, Nuria Magdalena Irisarri, Maximiliano Wappner, Pablo |
| author_facet |
Centanin, Lázaro Dekanty, Andrés Romero, Nuria Magdalena Irisarri, Maximiliano Wappner, Pablo |
| author_sort |
Centanin, Lázaro |
| title |
Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting |
| title_short |
Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting |
| title_full |
Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting |
| title_fullStr |
Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting |
| title_full_unstemmed |
Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting |
| title_sort |
cell autonomy of hif effects in drosophila: tracheal cells sense hypoxia and induce terminal branch sprouting |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15345807_v14_n4_p547_Centanin http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin |
| work_keys_str_mv |
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| _version_ |
1768545206068576256 |