Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata

Atrazine, a herbicide that is intensively used in Argentina, was assayed to evaluate the alteration of reproduction in a wild species of crustaceans. Adult females of the estuarine crab Neohelice granulata were exposed to formulated atrazine during the 3-month pre-reproductive period. Three atrazine...

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Autores principales:	Rodriguez, Enrique Marcelo, Medesani, Daniel Alberto
Publicado:	2017
Materias:	Atrazine Crabs Ovary Reproduction Vitellogenin atrazine glycogen vitellogenin herbicide water pollutant adult animal experiment animal tissue Argentina Article concentration (parameters) controlled study crab energy resource energy transfer estuarine species female glucose blood level gonadosomatic index hepatopancreas histology in vitro study molting mortality muscle nonhuman oocyte ovary development parameters priority journal reproductive toxicity spawning survival weight gain animal Brachyura dose response drug effects energy metabolism growth, development and aging metabolism ovary reproduction skeletal muscle time factor tissue culture technique toxicity Animals Dose-Response Relationship, Drug Energy Metabolism Female Glycogen Hepatopancreas Herbicides Muscle, Skeletal Oocytes Time Factors Tissue Culture Techniques Vitellogenins Water Pollutants, Chemical
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v192_n_p1_Silveyra http://hdl.handle.net/20.500.12110/paper_15320456_v192_n_p1_Silveyra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15320456_v192_n_p1_Silveyra
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spelling	paper:paper_15320456_v192_n_p1_Silveyra2023-06-08T16:19:56Z Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata Rodriguez, Enrique Marcelo Medesani, Daniel Alberto Atrazine Crabs Ovary Reproduction Vitellogenin atrazine glycogen vitellogenin atrazine glycogen herbicide vitellogenin water pollutant adult animal experiment animal tissue Argentina Article concentration (parameters) controlled study crab energy resource energy transfer estuarine species female glucose blood level gonadosomatic index hepatopancreas histology in vitro study molting mortality muscle nonhuman oocyte ovary development parameters priority journal reproductive toxicity spawning survival weight gain animal Brachyura dose response drug effects energy metabolism growth, development and aging metabolism ovary reproduction skeletal muscle time factor tissue culture technique toxicity water pollutant Animals Atrazine Brachyura Dose-Response Relationship, Drug Energy Metabolism Female Glycogen Hepatopancreas Herbicides Muscle, Skeletal Oocytes Ovary Reproduction Time Factors Tissue Culture Techniques Vitellogenins Water Pollutants, Chemical Atrazine, a herbicide that is intensively used in Argentina, was assayed to evaluate the alteration of reproduction in a wild species of crustaceans. Adult females of the estuarine crab Neohelice granulata were exposed to formulated atrazine during the 3-month pre-reproductive period. Three atrazine concentrations (0.03, 0.3 and 3 mg/L) were assayed, together with a water dilution control. At the end of the exposure period, several variables concerning the energetic status of animals were measured, such as weight gain, glycemia, and energy reserves in both muscle and hepatopancreas. The gonadosomatic index (GSI) was also determined, as well as the proportion and relative area of each oocyte type in histological sections. Besides, the total content of vitellogenin proteins (Vg) in both ovary and hepatopancreas was measured. A significant (p < 0.05) decrease of glycogen content was observed in muscle, while a significant (p < 0.05) lower area of both previtellogenic and vitellogenic oocytes was verified in the ovary by effect of atrazine, in correspondence with a Vg content significantly (p < 0.05) diminished in the ovary and augmented in the hepatopancreas. Besides, a higher proportion of previtellogenic oocytes were seen by effect of atrazine. Taken together, these results indicate a clear reduction and delay in the ovarian growth of the studied species, during the period at which the ovary normally grows up prior to spawning. On the other hand, a decrease of Vg content was observed at 0.3 and 3 mg/L of atrazine after 24-h in vitro assays carried out with ovarian explants, providing additional evidence about the inhibitory role of this herbicide on the ovarian growth. These results encourage future research on deleterious effects of atrazine on crustacean reproduction. © 2016 Elsevier Inc. Fil:Rodríguez, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Medesani, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v192_n_p1_Silveyra http://hdl.handle.net/20.500.12110/paper_15320456_v192_n_p1_Silveyra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Atrazine Crabs Ovary Reproduction Vitellogenin atrazine glycogen vitellogenin atrazine glycogen herbicide vitellogenin water pollutant adult animal experiment animal tissue Argentina Article concentration (parameters) controlled study crab energy resource energy transfer estuarine species female glucose blood level gonadosomatic index hepatopancreas histology in vitro study molting mortality muscle nonhuman oocyte ovary development parameters priority journal reproductive toxicity spawning survival weight gain animal Brachyura dose response drug effects energy metabolism growth, development and aging metabolism ovary reproduction skeletal muscle time factor tissue culture technique toxicity water pollutant Animals Atrazine Brachyura Dose-Response Relationship, Drug Energy Metabolism Female Glycogen Hepatopancreas Herbicides Muscle, Skeletal Oocytes Ovary Reproduction Time Factors Tissue Culture Techniques Vitellogenins Water Pollutants, Chemical
spellingShingle	Atrazine Crabs Ovary Reproduction Vitellogenin atrazine glycogen vitellogenin atrazine glycogen herbicide vitellogenin water pollutant adult animal experiment animal tissue Argentina Article concentration (parameters) controlled study crab energy resource energy transfer estuarine species female glucose blood level gonadosomatic index hepatopancreas histology in vitro study molting mortality muscle nonhuman oocyte ovary development parameters priority journal reproductive toxicity spawning survival weight gain animal Brachyura dose response drug effects energy metabolism growth, development and aging metabolism ovary reproduction skeletal muscle time factor tissue culture technique toxicity water pollutant Animals Atrazine Brachyura Dose-Response Relationship, Drug Energy Metabolism Female Glycogen Hepatopancreas Herbicides Muscle, Skeletal Oocytes Ovary Reproduction Time Factors Tissue Culture Techniques Vitellogenins Water Pollutants, Chemical Rodriguez, Enrique Marcelo Medesani, Daniel Alberto Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata
topic_facet	Atrazine Crabs Ovary Reproduction Vitellogenin atrazine glycogen vitellogenin atrazine glycogen herbicide vitellogenin water pollutant adult animal experiment animal tissue Argentina Article concentration (parameters) controlled study crab energy resource energy transfer estuarine species female glucose blood level gonadosomatic index hepatopancreas histology in vitro study molting mortality muscle nonhuman oocyte ovary development parameters priority journal reproductive toxicity spawning survival weight gain animal Brachyura dose response drug effects energy metabolism growth, development and aging metabolism ovary reproduction skeletal muscle time factor tissue culture technique toxicity water pollutant Animals Atrazine Brachyura Dose-Response Relationship, Drug Energy Metabolism Female Glycogen Hepatopancreas Herbicides Muscle, Skeletal Oocytes Ovary Reproduction Time Factors Tissue Culture Techniques Vitellogenins Water Pollutants, Chemical
description	Atrazine, a herbicide that is intensively used in Argentina, was assayed to evaluate the alteration of reproduction in a wild species of crustaceans. Adult females of the estuarine crab Neohelice granulata were exposed to formulated atrazine during the 3-month pre-reproductive period. Three atrazine concentrations (0.03, 0.3 and 3 mg/L) were assayed, together with a water dilution control. At the end of the exposure period, several variables concerning the energetic status of animals were measured, such as weight gain, glycemia, and energy reserves in both muscle and hepatopancreas. The gonadosomatic index (GSI) was also determined, as well as the proportion and relative area of each oocyte type in histological sections. Besides, the total content of vitellogenin proteins (Vg) in both ovary and hepatopancreas was measured. A significant (p < 0.05) decrease of glycogen content was observed in muscle, while a significant (p < 0.05) lower area of both previtellogenic and vitellogenic oocytes was verified in the ovary by effect of atrazine, in correspondence with a Vg content significantly (p < 0.05) diminished in the ovary and augmented in the hepatopancreas. Besides, a higher proportion of previtellogenic oocytes were seen by effect of atrazine. Taken together, these results indicate a clear reduction and delay in the ovarian growth of the studied species, during the period at which the ovary normally grows up prior to spawning. On the other hand, a decrease of Vg content was observed at 0.3 and 3 mg/L of atrazine after 24-h in vitro assays carried out with ovarian explants, providing additional evidence about the inhibitory role of this herbicide on the ovarian growth. These results encourage future research on deleterious effects of atrazine on crustacean reproduction. © 2016 Elsevier Inc.
author	Rodriguez, Enrique Marcelo Medesani, Daniel Alberto
author_facet	Rodriguez, Enrique Marcelo Medesani, Daniel Alberto
author_sort	Rodriguez, Enrique Marcelo
title	Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata
title_short	Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata
title_full	Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata
title_fullStr	Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata
title_full_unstemmed	Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata
title_sort	effects of atrazine on ovarian growth, in the estuarine crab neohelice granulata
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v192_n_p1_Silveyra http://hdl.handle.net/20.500.12110/paper_15320456_v192_n_p1_Silveyra
work_keys_str_mv	AT rodriguezenriquemarcelo effectsofatrazineonovariangrowthintheestuarinecrabneohelicegranulata AT medesanidanielalberto effectsofatrazineonovariangrowthintheestuarinecrabneohelicegranulata
_version_	1768542142252187648

Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata

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