Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus

The organochlorine pesticide endosulfan (ES) is used in several countries as a wide spectrum insecticide on crops with high commercial value. Due to its high toxicity to non-target animals, its persistence in the environment and its ability to act as an endocrine disrupting compound in fish, ES use...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Da Cuña, Rodrigo Hernán, Rey Vázquez, Graciela, Rodriguez, Enrique Marcelo, Lo Nostro, Fabiana Laura
Publicado:	2016
Materias:	Active ingredients Commercial formulation Endocrine disruption Endosulfan Gonad culture Organochlorine pesticide adenylate cyclase activator endosulfan estradiol forskolin hydroxyprogesterone luteinizing hormone luteinizing hormone receptor sex hormone steroid 17alpha monooxygenase testosterone adenylate cyclase endocrine disruptor enzyme activator water pollutant adult Article Cichlasoma dimerus cichlid comparative study controlled study estradiol blood level female gonad hormonal regulation hormone release in vitro study male nonhuman priority journal steroid release steroidogenesis testosterone blood level animal biosynthesis drug effects enzyme activation enzymology metabolism ovary signal transduction testis tissue culture technique toxicity Adenylyl Cyclases Animals Cichlids Colforsin Endocrine Disruptors Enzyme Activation Enzyme Activators Estradiol Female Luteinizing Hormone Male Ovary Signal Transduction Testis Testosterone Tissue Culture Techniques Water Pollutants, Chemical
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v187_n_p74_DaCuna http://hdl.handle.net/20.500.12110/paper_15320456_v187_n_p74_DaCuna
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15320456_v187_n_p74_DaCuna
record_format	dspace
spelling	paper:paper_15320456_v187_n_p74_DaCuna2023-06-08T16:19:55Z Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus Da Cuña, Rodrigo Hernán Rey Vázquez, Graciela Rodriguez, Enrique Marcelo Lo Nostro, Fabiana Laura Active ingredients Commercial formulation Endocrine disruption Endosulfan Gonad culture Organochlorine pesticide adenylate cyclase activator endosulfan estradiol forskolin hydroxyprogesterone luteinizing hormone luteinizing hormone receptor sex hormone steroid 17alpha monooxygenase testosterone adenylate cyclase endocrine disruptor endosulfan enzyme activator estradiol forskolin luteinizing hormone testosterone water pollutant adult Article Cichlasoma dimerus cichlid comparative study controlled study estradiol blood level female gonad hormonal regulation hormone release in vitro study male nonhuman priority journal steroid release steroidogenesis testosterone blood level animal biosynthesis cichlid drug effects enzyme activation enzymology metabolism ovary signal transduction testis tissue culture technique toxicity water pollutant Adenylyl Cyclases Animals Cichlids Colforsin Endocrine Disruptors Endosulfan Enzyme Activation Enzyme Activators Estradiol Female Luteinizing Hormone Male Ovary Signal Transduction Testis Testosterone Tissue Culture Techniques Water Pollutants, Chemical The organochlorine pesticide endosulfan (ES) is used in several countries as a wide spectrum insecticide on crops with high commercial value. Due to its high toxicity to non-target animals, its persistence in the environment and its ability to act as an endocrine disrupting compound in fish, ES use is currently banned or restricted in many other countries. Previous studies on the cichlid fish Cichlasoma dimerus have shown that waterborne exposure to ES can lead to both decreased pituitary FSH content and histological alterations of testes. As gonadotropin-stimulated sex steroids release from gonads was inhibited by ES in vitro, the aim of the present study was to elucidate possible mechanisms of disruption of ES on gonadal steroidogenesis in C. dimerus, as well as compare the action of the active ingredient (AI) with that of currently used commercial formulations (CF). Testis and ovary fragments were incubated with ES (AI or CF) and/or steroidogenesis activators or precursors. Testosterone and estradiol levels were measured in the incubation media. By itself, ES did not affect hormone levels. Co-incubation with LH and the adenylate cyclase activator forskolin caused a decrease of the stimulated sex steroids release. When co-incubated with precursors dehydroandrostenedione and 17αhydroxyprogesterone, ES did not affect the increase caused by their addition alone. No differences were observed between the AI and CFs, suggesting that the effect on steroidogenesis disruption is mainly caused by the AI. Results indicate that action of ES takes place downstream of LH-receptor activation and upstream of the studied steroidogenic enzymes. Fil:Da Cuña, R.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rey Vázquez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v187_n_p74_DaCuna http://hdl.handle.net/20.500.12110/paper_15320456_v187_n_p74_DaCuna
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Active ingredients Commercial formulation Endocrine disruption Endosulfan Gonad culture Organochlorine pesticide adenylate cyclase activator endosulfan estradiol forskolin hydroxyprogesterone luteinizing hormone luteinizing hormone receptor sex hormone steroid 17alpha monooxygenase testosterone adenylate cyclase endocrine disruptor endosulfan enzyme activator estradiol forskolin luteinizing hormone testosterone water pollutant adult Article Cichlasoma dimerus cichlid comparative study controlled study estradiol blood level female gonad hormonal regulation hormone release in vitro study male nonhuman priority journal steroid release steroidogenesis testosterone blood level animal biosynthesis cichlid drug effects enzyme activation enzymology metabolism ovary signal transduction testis tissue culture technique toxicity water pollutant Adenylyl Cyclases Animals Cichlids Colforsin Endocrine Disruptors Endosulfan Enzyme Activation Enzyme Activators Estradiol Female Luteinizing Hormone Male Ovary Signal Transduction Testis Testosterone Tissue Culture Techniques Water Pollutants, Chemical
spellingShingle	Active ingredients Commercial formulation Endocrine disruption Endosulfan Gonad culture Organochlorine pesticide adenylate cyclase activator endosulfan estradiol forskolin hydroxyprogesterone luteinizing hormone luteinizing hormone receptor sex hormone steroid 17alpha monooxygenase testosterone adenylate cyclase endocrine disruptor endosulfan enzyme activator estradiol forskolin luteinizing hormone testosterone water pollutant adult Article Cichlasoma dimerus cichlid comparative study controlled study estradiol blood level female gonad hormonal regulation hormone release in vitro study male nonhuman priority journal steroid release steroidogenesis testosterone blood level animal biosynthesis cichlid drug effects enzyme activation enzymology metabolism ovary signal transduction testis tissue culture technique toxicity water pollutant Adenylyl Cyclases Animals Cichlids Colforsin Endocrine Disruptors Endosulfan Enzyme Activation Enzyme Activators Estradiol Female Luteinizing Hormone Male Ovary Signal Transduction Testis Testosterone Tissue Culture Techniques Water Pollutants, Chemical Da Cuña, Rodrigo Hernán Rey Vázquez, Graciela Rodriguez, Enrique Marcelo Lo Nostro, Fabiana Laura Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus
topic_facet	Active ingredients Commercial formulation Endocrine disruption Endosulfan Gonad culture Organochlorine pesticide adenylate cyclase activator endosulfan estradiol forskolin hydroxyprogesterone luteinizing hormone luteinizing hormone receptor sex hormone steroid 17alpha monooxygenase testosterone adenylate cyclase endocrine disruptor endosulfan enzyme activator estradiol forskolin luteinizing hormone testosterone water pollutant adult Article Cichlasoma dimerus cichlid comparative study controlled study estradiol blood level female gonad hormonal regulation hormone release in vitro study male nonhuman priority journal steroid release steroidogenesis testosterone blood level animal biosynthesis cichlid drug effects enzyme activation enzymology metabolism ovary signal transduction testis tissue culture technique toxicity water pollutant Adenylyl Cyclases Animals Cichlids Colforsin Endocrine Disruptors Endosulfan Enzyme Activation Enzyme Activators Estradiol Female Luteinizing Hormone Male Ovary Signal Transduction Testis Testosterone Tissue Culture Techniques Water Pollutants, Chemical
description	The organochlorine pesticide endosulfan (ES) is used in several countries as a wide spectrum insecticide on crops with high commercial value. Due to its high toxicity to non-target animals, its persistence in the environment and its ability to act as an endocrine disrupting compound in fish, ES use is currently banned or restricted in many other countries. Previous studies on the cichlid fish Cichlasoma dimerus have shown that waterborne exposure to ES can lead to both decreased pituitary FSH content and histological alterations of testes. As gonadotropin-stimulated sex steroids release from gonads was inhibited by ES in vitro, the aim of the present study was to elucidate possible mechanisms of disruption of ES on gonadal steroidogenesis in C. dimerus, as well as compare the action of the active ingredient (AI) with that of currently used commercial formulations (CF). Testis and ovary fragments were incubated with ES (AI or CF) and/or steroidogenesis activators or precursors. Testosterone and estradiol levels were measured in the incubation media. By itself, ES did not affect hormone levels. Co-incubation with LH and the adenylate cyclase activator forskolin caused a decrease of the stimulated sex steroids release. When co-incubated with precursors dehydroandrostenedione and 17αhydroxyprogesterone, ES did not affect the increase caused by their addition alone. No differences were observed between the AI and CFs, suggesting that the effect on steroidogenesis disruption is mainly caused by the AI. Results indicate that action of ES takes place downstream of LH-receptor activation and upstream of the studied steroidogenic enzymes.
author	Da Cuña, Rodrigo Hernán Rey Vázquez, Graciela Rodriguez, Enrique Marcelo Lo Nostro, Fabiana Laura
author_facet	Da Cuña, Rodrigo Hernán Rey Vázquez, Graciela Rodriguez, Enrique Marcelo Lo Nostro, Fabiana Laura
author_sort	Da Cuña, Rodrigo Hernán
title	Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus
title_short	Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus
title_full	Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus
title_fullStr	Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus
title_full_unstemmed	Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus
title_sort	mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish cichlasoma dimerus
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v187_n_p74_DaCuna http://hdl.handle.net/20.500.12110/paper_15320456_v187_n_p74_DaCuna
work_keys_str_mv	AT dacunarodrigohernan mechanismofactionofendosulfanasdisruptorofgonadalsteroidogenesisinthecichlidfishcichlasomadimerus AT reyvazquezgraciela mechanismofactionofendosulfanasdisruptorofgonadalsteroidogenesisinthecichlidfishcichlasomadimerus AT rodriguezenriquemarcelo mechanismofactionofendosulfanasdisruptorofgonadalsteroidogenesisinthecichlidfishcichlasomadimerus AT lonostrofabianalaura mechanismofactionofendosulfanasdisruptorofgonadalsteroidogenesisinthecichlidfishcichlasomadimerus
_version_	1768546365643685888

Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus

Ejemplares similares