Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative...
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paper:paper_15320456_v179_n_p29_Poletta2023-06-08T16:19:55Z Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood Poletta, Gisela Laura Mudry, Marta Dolores Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative stress. No previous report existed on the application of oxidative damage and antioxidant defense biomarkers in Caiman latirostris blood, while few studies reported in other crocodilians were done in organs or muscles of dead animals. The aim of this study was to characterize a new set of oxidative stress biomarkers in C. latirostris blood, through the modification of conventional techniques: 1) damage to lipids by thiobarbituric acid reactive substances (TBARS), 2) damage to DNA by comet assay modified with the enzymes FPG and Endo III, and 3) antioxidant defenses: catalase, superoxide dismutase and glutathione; in order to apply them in future biomonitoring studies. We successfully adapted standard procedures for CAT, SOD, GSH and TBARS determination in C. latirostris blood. Calibration curves for FPG and Endo III showed that the three dilutions tested were appropriate to conduct the modified comet assay for the detection of oxidized bases in C. latirostris erythrocytes. One hour of incubation allowed a complete repair of the damage generated. The incorporation of these biomarkers in biomonitoring studies of caiman populations exposed to xenobiotics is highly important considering that this species has recovered from a serious endangered state through the implementation of sustainable use programs in Argentina, and represents nowadays a relevant economic resource for many human communities. © 2015 Elsevier Inc. Fil:Poletta, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v179_n_p29_Poletta http://hdl.handle.net/20.500.12110/paper_15320456_v179_n_p29_Poletta |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances |
spellingShingle |
Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances Poletta, Gisela Laura Mudry, Marta Dolores Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood |
topic_facet |
Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances |
description |
Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative stress. No previous report existed on the application of oxidative damage and antioxidant defense biomarkers in Caiman latirostris blood, while few studies reported in other crocodilians were done in organs or muscles of dead animals. The aim of this study was to characterize a new set of oxidative stress biomarkers in C. latirostris blood, through the modification of conventional techniques: 1) damage to lipids by thiobarbituric acid reactive substances (TBARS), 2) damage to DNA by comet assay modified with the enzymes FPG and Endo III, and 3) antioxidant defenses: catalase, superoxide dismutase and glutathione; in order to apply them in future biomonitoring studies. We successfully adapted standard procedures for CAT, SOD, GSH and TBARS determination in C. latirostris blood. Calibration curves for FPG and Endo III showed that the three dilutions tested were appropriate to conduct the modified comet assay for the detection of oxidized bases in C. latirostris erythrocytes. One hour of incubation allowed a complete repair of the damage generated. The incorporation of these biomarkers in biomonitoring studies of caiman populations exposed to xenobiotics is highly important considering that this species has recovered from a serious endangered state through the implementation of sustainable use programs in Argentina, and represents nowadays a relevant economic resource for many human communities. © 2015 Elsevier Inc. |
author |
Poletta, Gisela Laura Mudry, Marta Dolores |
author_facet |
Poletta, Gisela Laura Mudry, Marta Dolores |
author_sort |
Poletta, Gisela Laura |
title |
Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood |
title_short |
Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood |
title_full |
Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood |
title_fullStr |
Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood |
title_full_unstemmed |
Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood |
title_sort |
biomarkers of oxidative damage and antioxidant defense capacity in caiman latirostris blood |
publishDate |
2016 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v179_n_p29_Poletta http://hdl.handle.net/20.500.12110/paper_15320456_v179_n_p29_Poletta |
work_keys_str_mv |
AT polettagiselalaura biomarkersofoxidativedamageandantioxidantdefensecapacityincaimanlatirostrisblood AT mudrymartadolores biomarkersofoxidativedamageandantioxidantdefensecapacityincaimanlatirostrisblood |
_version_ |
1768545021786587136 |