Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates most of the toxic effects of environmental contaminants. Among the multiple pleiotropic responses elicited by AHR agonists, the antiestrogenic and endocrine-disrupting action of the receptor activation is on...

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Autores principales:	Bussmann, Ursula A., Barañao, José Lino S.
Publicado:	2013
Materias:	β-naphthoflavone Aryl hydrocarbon receptor Chicken hepatocytes Estrogen receptor LMH cell line Vitellogenin II gene alpha naphthoflavone aromatic hydrocarbon receptor beta naphthoflavone cytochrome P450 1A estradiol estrogen receptor vitellogenin animal cell animal experiment animal model animal tissue article chicken controlled study embryo gene expression hepatoma cell liver cell liver cell carcinoma nonhuman priority journal promoter region transcription initiation transient transfection Animals Aryl Hydrocarbon Hydroxylases Avian Proteins beta-Naphthoflavone Cell Line, Tumor Cells, Cultured Chick Embryo Chickens Enzyme Inhibitors Estradiol Estrogens Fluorescent Antibody Technique Gene Expression Microscopy, Confocal Promoter Regions, Genetic Protein Binding Receptors, Aryl Hydrocarbon Receptors, Estrogen Reverse Transcriptase Polymerase Chain Reaction Vitellogenins Aves
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v157_n2_p111_Bussmann http://hdl.handle.net/20.500.12110/paper_15320456_v157_n2_p111_Bussmann
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_15320456_v157_n2_p111_Bussmann2023-06-08T16:19:55Z Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells Bussmann, Ursula A. Barañao, José Lino S. β-naphthoflavone Aryl hydrocarbon receptor Chicken hepatocytes Estrogen receptor LMH cell line Vitellogenin II gene alpha naphthoflavone aromatic hydrocarbon receptor beta naphthoflavone cytochrome P450 1A estradiol estrogen receptor vitellogenin animal cell animal experiment animal model animal tissue article chicken controlled study embryo gene expression hepatoma cell liver cell liver cell carcinoma nonhuman priority journal promoter region transcription initiation transient transfection Animals Aryl Hydrocarbon Hydroxylases Avian Proteins beta-Naphthoflavone Cell Line, Tumor Cells, Cultured Chick Embryo Chickens Enzyme Inhibitors Estradiol Estrogens Fluorescent Antibody Technique Gene Expression Microscopy, Confocal Promoter Regions, Genetic Protein Binding Receptors, Aryl Hydrocarbon Receptors, Estrogen Reverse Transcriptase Polymerase Chain Reaction Vitellogenins Aves The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates most of the toxic effects of environmental contaminants. Among the multiple pleiotropic responses elicited by AHR agonists, the antiestrogenic and endocrine-disrupting action of the receptor activation is one of the most studied. It has been demonstrated that some AHR agonists disrupt estradiol-induced vitellogenin synthesis in the fish liver via a mechanism that involves crosstalk between the AHR and the estrogen receptor (ER). Chicken hepatocytes have become a model for the study of AHR action in birds and the induction of the signal and its effect in these cells are well established. However, the impact of AHR activation on estradiol-regulated responses in the chicken liver remains to be demonstrated. The aim of the present study was, therefore, to determine the effect of AHR action on ER-driven transcription in a convenient model of chicken liver cells. For this purpose, we designed a reporter construct bearing the 5′ regulatory region of the chicken vitellogenin II gene and used it to transfect chicken hepatoma LMH cells. We found that β-naphthoflavone represses ER-driven vitellogenin promoter activity and that this action is mediated by the AHR. This inhibitory crosstalk between both pathways appears to be unidirectional, since estradiol did not alter the transcript levels of an AHR target gene. Besides, and highly relevant, we show that LMH cell line transfected with a reporter construct bearing the chicken vitellogenin promoter sequence is a useful and convenient model for the study of AHR-ER interaction in chicken liver-derived cells. © 2012 Elsevier Inc. Fil:Bussmann, U.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v157_n2_p111_Bussmann http://hdl.handle.net/20.500.12110/paper_15320456_v157_n2_p111_Bussmann
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-naphthoflavone Aryl hydrocarbon receptor Chicken hepatocytes Estrogen receptor LMH cell line Vitellogenin II gene alpha naphthoflavone aromatic hydrocarbon receptor beta naphthoflavone cytochrome P450 1A estradiol estrogen receptor vitellogenin animal cell animal experiment animal model animal tissue article chicken controlled study embryo gene expression hepatoma cell liver cell liver cell carcinoma nonhuman priority journal promoter region transcription initiation transient transfection Animals Aryl Hydrocarbon Hydroxylases Avian Proteins beta-Naphthoflavone Cell Line, Tumor Cells, Cultured Chick Embryo Chickens Enzyme Inhibitors Estradiol Estrogens Fluorescent Antibody Technique Gene Expression Microscopy, Confocal Promoter Regions, Genetic Protein Binding Receptors, Aryl Hydrocarbon Receptors, Estrogen Reverse Transcriptase Polymerase Chain Reaction Vitellogenins Aves
spellingShingle	β-naphthoflavone Aryl hydrocarbon receptor Chicken hepatocytes Estrogen receptor LMH cell line Vitellogenin II gene alpha naphthoflavone aromatic hydrocarbon receptor beta naphthoflavone cytochrome P450 1A estradiol estrogen receptor vitellogenin animal cell animal experiment animal model animal tissue article chicken controlled study embryo gene expression hepatoma cell liver cell liver cell carcinoma nonhuman priority journal promoter region transcription initiation transient transfection Animals Aryl Hydrocarbon Hydroxylases Avian Proteins beta-Naphthoflavone Cell Line, Tumor Cells, Cultured Chick Embryo Chickens Enzyme Inhibitors Estradiol Estrogens Fluorescent Antibody Technique Gene Expression Microscopy, Confocal Promoter Regions, Genetic Protein Binding Receptors, Aryl Hydrocarbon Receptors, Estrogen Reverse Transcriptase Polymerase Chain Reaction Vitellogenins Aves Bussmann, Ursula A. Barañao, José Lino S. Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells
topic_facet	β-naphthoflavone Aryl hydrocarbon receptor Chicken hepatocytes Estrogen receptor LMH cell line Vitellogenin II gene alpha naphthoflavone aromatic hydrocarbon receptor beta naphthoflavone cytochrome P450 1A estradiol estrogen receptor vitellogenin animal cell animal experiment animal model animal tissue article chicken controlled study embryo gene expression hepatoma cell liver cell liver cell carcinoma nonhuman priority journal promoter region transcription initiation transient transfection Animals Aryl Hydrocarbon Hydroxylases Avian Proteins beta-Naphthoflavone Cell Line, Tumor Cells, Cultured Chick Embryo Chickens Enzyme Inhibitors Estradiol Estrogens Fluorescent Antibody Technique Gene Expression Microscopy, Confocal Promoter Regions, Genetic Protein Binding Receptors, Aryl Hydrocarbon Receptors, Estrogen Reverse Transcriptase Polymerase Chain Reaction Vitellogenins Aves
description	The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates most of the toxic effects of environmental contaminants. Among the multiple pleiotropic responses elicited by AHR agonists, the antiestrogenic and endocrine-disrupting action of the receptor activation is one of the most studied. It has been demonstrated that some AHR agonists disrupt estradiol-induced vitellogenin synthesis in the fish liver via a mechanism that involves crosstalk between the AHR and the estrogen receptor (ER). Chicken hepatocytes have become a model for the study of AHR action in birds and the induction of the signal and its effect in these cells are well established. However, the impact of AHR activation on estradiol-regulated responses in the chicken liver remains to be demonstrated. The aim of the present study was, therefore, to determine the effect of AHR action on ER-driven transcription in a convenient model of chicken liver cells. For this purpose, we designed a reporter construct bearing the 5′ regulatory region of the chicken vitellogenin II gene and used it to transfect chicken hepatoma LMH cells. We found that β-naphthoflavone represses ER-driven vitellogenin promoter activity and that this action is mediated by the AHR. This inhibitory crosstalk between both pathways appears to be unidirectional, since estradiol did not alter the transcript levels of an AHR target gene. Besides, and highly relevant, we show that LMH cell line transfected with a reporter construct bearing the chicken vitellogenin promoter sequence is a useful and convenient model for the study of AHR-ER interaction in chicken liver-derived cells. © 2012 Elsevier Inc.
author	Bussmann, Ursula A. Barañao, José Lino S.
author_facet	Bussmann, Ursula A. Barañao, José Lino S.
author_sort	Bussmann, Ursula A.
title	Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells
title_short	Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells
title_full	Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells
title_fullStr	Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells
title_full_unstemmed	Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells
title_sort	aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in lmh cells
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v157_n2_p111_Bussmann http://hdl.handle.net/20.500.12110/paper_15320456_v157_n2_p111_Bussmann
work_keys_str_mv	AT bussmannursulaa arylhydrocarbonreceptoractivationleadstoimpairmentofestrogendrivenchickenvitellogeninpromoteractivityinlmhcells AT baranaojoselinos arylhydrocarbonreceptoractivationleadstoimpairmentofestrogendrivenchickenvitellogeninpromoteractivityinlmhcells
_version_	1768545113535938560

Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells

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