Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes)
Exposure to environmental pollutants may disrupt endocrine functions and cause reproductive effects in human and wildlife populations. Various groups of chemicals have estrogen-like effects, including degradation products of alkylphenol polyethoxylates, such as 4-tert-octylphenol (OP). Laboratory st...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Publicado: |
2009
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v150_n2_p298_ReyVazquez http://hdl.handle.net/20.500.12110/paper_15320456_v150_n2_p298_ReyVazquez |
| Aporte de: |
| id |
paper:paper_15320456_v150_n2_p298_ReyVazquez |
|---|---|
| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cichlasoma dimerus Cichlids Liver and testis pathology Octylphenol Surface mucus Teleosts Vitellogenin Xenoestrogens 4 tert octylphenol biological marker endocrine disruptor estrogen vitellogenin animal experiment animal tissue article cell ultrastructure Cichlasoma dimerus controlled study dot hybridization environmental exposure female fibrosis freshwater fish immunoblotting immunohistochemistry liver cell male microscopy mucus secretion nonhuman Perciformes priority journal protein blood level protein induction spermatogenesis surface mucus teleost testis development Western blotting Animals Argentina Biological Markers Dose-Response Relationship, Drug Endocrine Disruptors Environmental Monitoring Female Fibrosis Fish Proteins Fresh Water Hepatocytes Liver Male Mucus Perciformes Phenols Spermatogenesis Testis Time Factors Vitellogenins Water Pollutants, Chemical Cichlasoma Cichlidae Perciformes Teleostei |
| spellingShingle |
Cichlasoma dimerus Cichlids Liver and testis pathology Octylphenol Surface mucus Teleosts Vitellogenin Xenoestrogens 4 tert octylphenol biological marker endocrine disruptor estrogen vitellogenin animal experiment animal tissue article cell ultrastructure Cichlasoma dimerus controlled study dot hybridization environmental exposure female fibrosis freshwater fish immunoblotting immunohistochemistry liver cell male microscopy mucus secretion nonhuman Perciformes priority journal protein blood level protein induction spermatogenesis surface mucus teleost testis development Western blotting Animals Argentina Biological Markers Dose-Response Relationship, Drug Endocrine Disruptors Environmental Monitoring Female Fibrosis Fish Proteins Fresh Water Hepatocytes Liver Male Mucus Perciformes Phenols Spermatogenesis Testis Time Factors Vitellogenins Water Pollutants, Chemical Cichlasoma Cichlidae Perciformes Teleostei Rey Vázquez, Graciela Meijide, Fernando Javier Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura Piazza, Yanina Grisel Babay, Paola Alejandra Maggese, María Cristina Irene Guerrero, Graciela Alicia Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) |
| topic_facet |
Cichlasoma dimerus Cichlids Liver and testis pathology Octylphenol Surface mucus Teleosts Vitellogenin Xenoestrogens 4 tert octylphenol biological marker endocrine disruptor estrogen vitellogenin animal experiment animal tissue article cell ultrastructure Cichlasoma dimerus controlled study dot hybridization environmental exposure female fibrosis freshwater fish immunoblotting immunohistochemistry liver cell male microscopy mucus secretion nonhuman Perciformes priority journal protein blood level protein induction spermatogenesis surface mucus teleost testis development Western blotting Animals Argentina Biological Markers Dose-Response Relationship, Drug Endocrine Disruptors Environmental Monitoring Female Fibrosis Fish Proteins Fresh Water Hepatocytes Liver Male Mucus Perciformes Phenols Spermatogenesis Testis Time Factors Vitellogenins Water Pollutants, Chemical Cichlasoma Cichlidae Perciformes Teleostei |
| description |
Exposure to environmental pollutants may disrupt endocrine functions and cause reproductive effects in human and wildlife populations. Various groups of chemicals have estrogen-like effects, including degradation products of alkylphenol polyethoxylates, such as 4-tert-octylphenol (OP). Laboratory studies have shown that exposure of male fish to xenoestrogens results in induction of circulating vitellogenin (Vtg), inhibition of testicular growth, testis abnormalities and formation of intersex gonads. In this study, the impact of the exposure to waterborne OP on reproductive aspects in the South American cichlid fish Cichlasoma dimerus was evaluated using qualitative changes in the levels of Vtg in plasma and surface mucus and histological alterations in the liver and gonads as endpoints. Adult males and females were exposed to OP via immersion during 60 days in aquaria under semi-static conditions, water changes being made every 84 h. Treatment groups were: control (ethanol 0.005%), OP 30, 150 and 300 μg/L. Using Western and Dot blot analysis, Vtg was detected in plasma and mucus of control and treated females and treated males, while no Vtg was observed in samples from control males. Morphological changes in the hepatocytes due to the accumulation of Vtg were observed in OP-exposed males. Impairment of testicular structure became apparent in males treated with the highest OP concentrations. The most salient pathological change was the alteration of lobular organization with increased testicular fibrosis and progressive disruption of spermatogenesis. No major changes were observed in ovarian architecture. Our results indicate that detection of Vtg in surface mucus may be a sensitive and non-invasive biomarker of the endocrine disrupting effects of environmental estrogens, resulting in a useful method for field monitoring. © 2009 Elsevier Inc. All rights reserved. |
| author |
Rey Vázquez, Graciela Meijide, Fernando Javier Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura Piazza, Yanina Grisel Babay, Paola Alejandra Maggese, María Cristina Irene Guerrero, Graciela Alicia |
| author_facet |
Rey Vázquez, Graciela Meijide, Fernando Javier Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura Piazza, Yanina Grisel Babay, Paola Alejandra Maggese, María Cristina Irene Guerrero, Graciela Alicia |
| author_sort |
Rey Vázquez, Graciela |
| title |
Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) |
| title_short |
Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) |
| title_full |
Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) |
| title_fullStr |
Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) |
| title_full_unstemmed |
Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) |
| title_sort |
exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the south american freshwater fish cichlasoma dimerus (teleostei, perciformes) |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v150_n2_p298_ReyVazquez http://hdl.handle.net/20.500.12110/paper_15320456_v150_n2_p298_ReyVazquez |
| work_keys_str_mv |
AT reyvazquezgraciela exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes AT meijidefernandojavier exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes AT dacunarodrigohernan exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes AT lonostrofabianalaura exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes AT piazzayaninagrisel exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes AT babaypaolaalejandra exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes AT maggesemariacristinairene exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes AT guerrerogracielaalicia exposuretowaterborne4tertoctylphenolinducesvitellogeninsynthesisanddisruptstestismorphologyinthesouthamericanfreshwaterfishcichlasomadimerusteleosteiperciformes |
| _version_ |
1768543625565700096 |
| spelling |
paper:paper_15320456_v150_n2_p298_ReyVazquez2023-06-08T16:19:54Z Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) Rey Vázquez, Graciela Meijide, Fernando Javier Da Cuña, Rodrigo Hernán Lo Nostro, Fabiana Laura Piazza, Yanina Grisel Babay, Paola Alejandra Maggese, María Cristina Irene Guerrero, Graciela Alicia Cichlasoma dimerus Cichlids Liver and testis pathology Octylphenol Surface mucus Teleosts Vitellogenin Xenoestrogens 4 tert octylphenol biological marker endocrine disruptor estrogen vitellogenin animal experiment animal tissue article cell ultrastructure Cichlasoma dimerus controlled study dot hybridization environmental exposure female fibrosis freshwater fish immunoblotting immunohistochemistry liver cell male microscopy mucus secretion nonhuman Perciformes priority journal protein blood level protein induction spermatogenesis surface mucus teleost testis development Western blotting Animals Argentina Biological Markers Dose-Response Relationship, Drug Endocrine Disruptors Environmental Monitoring Female Fibrosis Fish Proteins Fresh Water Hepatocytes Liver Male Mucus Perciformes Phenols Spermatogenesis Testis Time Factors Vitellogenins Water Pollutants, Chemical Cichlasoma Cichlidae Perciformes Teleostei Exposure to environmental pollutants may disrupt endocrine functions and cause reproductive effects in human and wildlife populations. Various groups of chemicals have estrogen-like effects, including degradation products of alkylphenol polyethoxylates, such as 4-tert-octylphenol (OP). Laboratory studies have shown that exposure of male fish to xenoestrogens results in induction of circulating vitellogenin (Vtg), inhibition of testicular growth, testis abnormalities and formation of intersex gonads. In this study, the impact of the exposure to waterborne OP on reproductive aspects in the South American cichlid fish Cichlasoma dimerus was evaluated using qualitative changes in the levels of Vtg in plasma and surface mucus and histological alterations in the liver and gonads as endpoints. Adult males and females were exposed to OP via immersion during 60 days in aquaria under semi-static conditions, water changes being made every 84 h. Treatment groups were: control (ethanol 0.005%), OP 30, 150 and 300 μg/L. Using Western and Dot blot analysis, Vtg was detected in plasma and mucus of control and treated females and treated males, while no Vtg was observed in samples from control males. Morphological changes in the hepatocytes due to the accumulation of Vtg were observed in OP-exposed males. Impairment of testicular structure became apparent in males treated with the highest OP concentrations. The most salient pathological change was the alteration of lobular organization with increased testicular fibrosis and progressive disruption of spermatogenesis. No major changes were observed in ovarian architecture. Our results indicate that detection of Vtg in surface mucus may be a sensitive and non-invasive biomarker of the endocrine disrupting effects of environmental estrogens, resulting in a useful method for field monitoring. © 2009 Elsevier Inc. All rights reserved. Fil:Rey Vázquez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Meijide, F.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Da Cuña, R.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piazza, Y.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Babay, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerrero, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v150_n2_p298_ReyVazquez http://hdl.handle.net/20.500.12110/paper_15320456_v150_n2_p298_ReyVazquez |