Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae)
Microcystins are toxins produced by cyanobacteria, being toxic to aquatic fauna. It was evaluated alternative mechanisms of microcystins toxicity, including oxidative stress and histopathology in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae). Microcystins wa...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v135_n4_p459_Pinho http://hdl.handle.net/20.500.12110/paper_15320456_v135_n4_p459_Pinho |
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paper:paper_15320456_v135_n4_p459_Pinho2023-06-08T16:19:53Z Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae) Luquet, Carlos Marcelo Monserrat, José María B cells Catalase Chasmagnathus granulatus Estuarine crab Glutathione-S-transferase Histopathology Microcystins Oxidative stress catalase cyanoginosin glutathione transferase superoxide dismutase animal experiment animal model animal tissue article B lymphocyte controlled study crab enzyme activity hepatopancreas histopathology lipid peroxidation liver toxicity lymphocyte count male minimum inhibitory concentration nonhuman oxidative stress priority journal Animals Bacterial Toxins Catalase Cyanobacteria Decapoda (Crustacea) Glutathione Transferase Hepatopancreas Histocytochemistry Lipid Peroxides Male Melanins Microcystins Necrosis Oxidative Stress Peptides, Cyclic Superoxide Dismutase Chasmagnathus granulata Cyanobacteria Decapoda (Crustacea) Grapsidae Microcystins are toxins produced by cyanobacteria, being toxic to aquatic fauna. It was evaluated alternative mechanisms of microcystins toxicity, including oxidative stress and histopathology in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae). Microcystins was administered to crabs (MIC group) over 1 week, whereas the control (CTR group) received the saline from cyanobacteria culture medium. At day 7, catalase activity was higher in the MIC than in the CTR group, although a decrease of activity was verified in both groups with respect to time 0. Glutathione-S-transferase activity augmented in MIC with respect to CTR, suggesting a higher conjugation rate of the toxins with glutathione. No differences were detected in the superoxide dismutase activity. Lipid peroxidation remained stable in both groups. Histopathological analyses showed that the number of B cells decreased significantly in the CTR as a possible effect of starvation, while no significant change was observed in the MIC group. The hepatopancreas from the MIC group exhibited some necrotic tubules and melanin-like deposits. Overall, results showed that some enzymes of the antioxidant defense system were activated after microcystins exposure, this response being able to maintain lipid peroxidation levels, but insufficient to completely prevent histological damage. © 2003 Elsevier Inc. All rights reserved. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Monserrat, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v135_n4_p459_Pinho http://hdl.handle.net/20.500.12110/paper_15320456_v135_n4_p459_Pinho |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
B cells Catalase Chasmagnathus granulatus Estuarine crab Glutathione-S-transferase Histopathology Microcystins Oxidative stress catalase cyanoginosin glutathione transferase superoxide dismutase animal experiment animal model animal tissue article B lymphocyte controlled study crab enzyme activity hepatopancreas histopathology lipid peroxidation liver toxicity lymphocyte count male minimum inhibitory concentration nonhuman oxidative stress priority journal Animals Bacterial Toxins Catalase Cyanobacteria Decapoda (Crustacea) Glutathione Transferase Hepatopancreas Histocytochemistry Lipid Peroxides Male Melanins Microcystins Necrosis Oxidative Stress Peptides, Cyclic Superoxide Dismutase Chasmagnathus granulata Cyanobacteria Decapoda (Crustacea) Grapsidae |
| spellingShingle |
B cells Catalase Chasmagnathus granulatus Estuarine crab Glutathione-S-transferase Histopathology Microcystins Oxidative stress catalase cyanoginosin glutathione transferase superoxide dismutase animal experiment animal model animal tissue article B lymphocyte controlled study crab enzyme activity hepatopancreas histopathology lipid peroxidation liver toxicity lymphocyte count male minimum inhibitory concentration nonhuman oxidative stress priority journal Animals Bacterial Toxins Catalase Cyanobacteria Decapoda (Crustacea) Glutathione Transferase Hepatopancreas Histocytochemistry Lipid Peroxides Male Melanins Microcystins Necrosis Oxidative Stress Peptides, Cyclic Superoxide Dismutase Chasmagnathus granulata Cyanobacteria Decapoda (Crustacea) Grapsidae Luquet, Carlos Marcelo Monserrat, José María Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae) |
| topic_facet |
B cells Catalase Chasmagnathus granulatus Estuarine crab Glutathione-S-transferase Histopathology Microcystins Oxidative stress catalase cyanoginosin glutathione transferase superoxide dismutase animal experiment animal model animal tissue article B lymphocyte controlled study crab enzyme activity hepatopancreas histopathology lipid peroxidation liver toxicity lymphocyte count male minimum inhibitory concentration nonhuman oxidative stress priority journal Animals Bacterial Toxins Catalase Cyanobacteria Decapoda (Crustacea) Glutathione Transferase Hepatopancreas Histocytochemistry Lipid Peroxides Male Melanins Microcystins Necrosis Oxidative Stress Peptides, Cyclic Superoxide Dismutase Chasmagnathus granulata Cyanobacteria Decapoda (Crustacea) Grapsidae |
| description |
Microcystins are toxins produced by cyanobacteria, being toxic to aquatic fauna. It was evaluated alternative mechanisms of microcystins toxicity, including oxidative stress and histopathology in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae). Microcystins was administered to crabs (MIC group) over 1 week, whereas the control (CTR group) received the saline from cyanobacteria culture medium. At day 7, catalase activity was higher in the MIC than in the CTR group, although a decrease of activity was verified in both groups with respect to time 0. Glutathione-S-transferase activity augmented in MIC with respect to CTR, suggesting a higher conjugation rate of the toxins with glutathione. No differences were detected in the superoxide dismutase activity. Lipid peroxidation remained stable in both groups. Histopathological analyses showed that the number of B cells decreased significantly in the CTR as a possible effect of starvation, while no significant change was observed in the MIC group. The hepatopancreas from the MIC group exhibited some necrotic tubules and melanin-like deposits. Overall, results showed that some enzymes of the antioxidant defense system were activated after microcystins exposure, this response being able to maintain lipid peroxidation levels, but insufficient to completely prevent histological damage. © 2003 Elsevier Inc. All rights reserved. |
| author |
Luquet, Carlos Marcelo Monserrat, José María |
| author_facet |
Luquet, Carlos Marcelo Monserrat, José María |
| author_sort |
Luquet, Carlos Marcelo |
| title |
Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae) |
| title_short |
Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae) |
| title_full |
Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae) |
| title_fullStr |
Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae) |
| title_full_unstemmed |
Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae) |
| title_sort |
toxic effects of microcystins in the hepatopancreas of the estuarine crab chasmagnathus granulatus (decapoda, grapsidae) |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v135_n4_p459_Pinho http://hdl.handle.net/20.500.12110/paper_15320456_v135_n4_p459_Pinho |
| work_keys_str_mv |
AT luquetcarlosmarcelo toxiceffectsofmicrocystinsinthehepatopancreasoftheestuarinecrabchasmagnathusgranulatusdecapodagrapsidae AT monserratjosemaria toxiceffectsofmicrocystinsinthehepatopancreasoftheestuarinecrabchasmagnathusgranulatusdecapodagrapsidae |
| _version_ |
1768545945072435200 |