Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons
To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections rev...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1526954X_v36_n4_p196_Gelman http://hdl.handle.net/20.500.12110/paper_1526954X_v36_n4_p196_Gelman |
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paper:paper_1526954X_v36_n4_p196_Gelman2023-06-08T16:19:39Z Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons Catecholamine Cre recombinase Dopamine LoxP Norepinephrine Transgenic mouse Tyrosine hydroxylase catecholamine cre recombinase dopamine gene product protein cre loxp recombinant DNA tyrosine 3 monooxygenase unclassified drug adrenal medulla animal cell article brain region catecholamine nerve cell chromaffin cell DNA recombination dopaminergic nerve cell double immunohistochemistry floxed gene gene function gene mutation gene targeting gene technology genetic engineering hypothalamus immunofluorescence immunohistochemistry locus ceruleus mesencephalon mouse nonhuman olfactory bulb priority journal promoter region protein expression protein localization rat retina amacrine cell strain identification sympathetic ganglion transgenic mouse Alkaline Phosphatase Animals Brain Chemistry Catecholamines Female Gene Expression Regulation, Enzymologic Gene Targeting Genes, Reporter Genetic Engineering Humans Immunohistochemistry Integrases Mice Mice, Transgenic Neurons Pregnancy Promoter Regions (Genetics) Rats Recombination, Genetic Tissue Distribution Transgenes Tyrosine 3-Monooxygenase Viral Proteins Mus musculus To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH-positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We intercrossed TH-Cre mice with the floxed reporter strain Z/AP and observed efficient Cre-mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of "floxed" genes in catecholaminergic neurons. © 2003 Wiley-Liss, Inc. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1526954X_v36_n4_p196_Gelman http://hdl.handle.net/20.500.12110/paper_1526954X_v36_n4_p196_Gelman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Catecholamine Cre recombinase Dopamine LoxP Norepinephrine Transgenic mouse Tyrosine hydroxylase catecholamine cre recombinase dopamine gene product protein cre loxp recombinant DNA tyrosine 3 monooxygenase unclassified drug adrenal medulla animal cell article brain region catecholamine nerve cell chromaffin cell DNA recombination dopaminergic nerve cell double immunohistochemistry floxed gene gene function gene mutation gene targeting gene technology genetic engineering hypothalamus immunofluorescence immunohistochemistry locus ceruleus mesencephalon mouse nonhuman olfactory bulb priority journal promoter region protein expression protein localization rat retina amacrine cell strain identification sympathetic ganglion transgenic mouse Alkaline Phosphatase Animals Brain Chemistry Catecholamines Female Gene Expression Regulation, Enzymologic Gene Targeting Genes, Reporter Genetic Engineering Humans Immunohistochemistry Integrases Mice Mice, Transgenic Neurons Pregnancy Promoter Regions (Genetics) Rats Recombination, Genetic Tissue Distribution Transgenes Tyrosine 3-Monooxygenase Viral Proteins Mus musculus |
| spellingShingle |
Catecholamine Cre recombinase Dopamine LoxP Norepinephrine Transgenic mouse Tyrosine hydroxylase catecholamine cre recombinase dopamine gene product protein cre loxp recombinant DNA tyrosine 3 monooxygenase unclassified drug adrenal medulla animal cell article brain region catecholamine nerve cell chromaffin cell DNA recombination dopaminergic nerve cell double immunohistochemistry floxed gene gene function gene mutation gene targeting gene technology genetic engineering hypothalamus immunofluorescence immunohistochemistry locus ceruleus mesencephalon mouse nonhuman olfactory bulb priority journal promoter region protein expression protein localization rat retina amacrine cell strain identification sympathetic ganglion transgenic mouse Alkaline Phosphatase Animals Brain Chemistry Catecholamines Female Gene Expression Regulation, Enzymologic Gene Targeting Genes, Reporter Genetic Engineering Humans Immunohistochemistry Integrases Mice Mice, Transgenic Neurons Pregnancy Promoter Regions (Genetics) Rats Recombination, Genetic Tissue Distribution Transgenes Tyrosine 3-Monooxygenase Viral Proteins Mus musculus Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons |
| topic_facet |
Catecholamine Cre recombinase Dopamine LoxP Norepinephrine Transgenic mouse Tyrosine hydroxylase catecholamine cre recombinase dopamine gene product protein cre loxp recombinant DNA tyrosine 3 monooxygenase unclassified drug adrenal medulla animal cell article brain region catecholamine nerve cell chromaffin cell DNA recombination dopaminergic nerve cell double immunohistochemistry floxed gene gene function gene mutation gene targeting gene technology genetic engineering hypothalamus immunofluorescence immunohistochemistry locus ceruleus mesencephalon mouse nonhuman olfactory bulb priority journal promoter region protein expression protein localization rat retina amacrine cell strain identification sympathetic ganglion transgenic mouse Alkaline Phosphatase Animals Brain Chemistry Catecholamines Female Gene Expression Regulation, Enzymologic Gene Targeting Genes, Reporter Genetic Engineering Humans Immunohistochemistry Integrases Mice Mice, Transgenic Neurons Pregnancy Promoter Regions (Genetics) Rats Recombination, Genetic Tissue Distribution Transgenes Tyrosine 3-Monooxygenase Viral Proteins Mus musculus |
| description |
To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH-positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We intercrossed TH-Cre mice with the floxed reporter strain Z/AP and observed efficient Cre-mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of "floxed" genes in catecholaminergic neurons. © 2003 Wiley-Liss, Inc. |
| title |
Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons |
| title_short |
Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons |
| title_full |
Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons |
| title_fullStr |
Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons |
| title_full_unstemmed |
Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons |
| title_sort |
transgenic mice engineered to target cre/loxp-mediated dna recombination into catecholaminergic neurons |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1526954X_v36_n4_p196_Gelman http://hdl.handle.net/20.500.12110/paper_1526954X_v36_n4_p196_Gelman |
| _version_ |
1768546555029094400 |