Formulation and drying of alginate beads for controlled release and stabilization of invertase
Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (...
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2011
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paper:paper_15257797_v12_n9_p3147_Santagapita2023-06-08T16:19:35Z Formulation and drying of alginate beads for controlled release and stabilization of invertase Santagapita, Patricio Román Mazzobre, María Florencia Buera, María del Pilar Air drying Alginate beads Bead structures Composition changes Controlled release Drying methods Enzyme release Fickian diffusion Polymer chains Release kinetics Release mechanism Coagulation Enzyme activity Freezing Gelation Heat treatment Vacuum Drying alginate bead alginic acid beta cyclodextrin beta fructofuranosidase pectin trehalose unclassified drug article chemical composition chemical structure desiccation diffusion encapsulation enzyme activity enzyme kinetics enzyme release enzyme stability freeze drying freezing gelation heat treatment pH porosity priority journal vacuum Alginates beta-Cyclodextrins beta-Fructofuranosidase Calorimetry, Differential Scanning Delayed-Action Preparations Desiccation Drug Carriers Enzyme Stability Freeze Drying Gels Hydrogen-Ion Concentration Microscopy, Electron, Scanning Pectins Solubility Trehalose Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or air drying), and thermal treatment were evaluated on invertase stability and its release kinetics from beads. The enzyme release mechanism from wet beads depended on pH. The addition of trehalose, pectin, and β-cyclodextrin modified the bead structure, leading in some cases to a release mechanism that included the relaxation of the polymer chains, besides Fickian diffusion. Enzyme release from vacuum-dried beads was much faster than from freeze-dried beads, probably due to their higher pore size. The inclusion of β-cyclodextrin and especially of pectin prevented enzyme activity losses during bead generation, and trehalose addition was fundamental for achieving adequate invertase protection during freezing, drying, and thermal treatment. Present results showed that several alternatives such as drying method, composition, as well as pH of the relese medium can be managed to control enzyme release. © 2011 American Chemical Society. Fil:Santagapita, P.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzobre, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buera, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15257797_v12_n9_p3147_Santagapita http://hdl.handle.net/20.500.12110/paper_15257797_v12_n9_p3147_Santagapita |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Air drying Alginate beads Bead structures Composition changes Controlled release Drying methods Enzyme release Fickian diffusion Polymer chains Release kinetics Release mechanism Coagulation Enzyme activity Freezing Gelation Heat treatment Vacuum Drying alginate bead alginic acid beta cyclodextrin beta fructofuranosidase pectin trehalose unclassified drug article chemical composition chemical structure desiccation diffusion encapsulation enzyme activity enzyme kinetics enzyme release enzyme stability freeze drying freezing gelation heat treatment pH porosity priority journal vacuum Alginates beta-Cyclodextrins beta-Fructofuranosidase Calorimetry, Differential Scanning Delayed-Action Preparations Desiccation Drug Carriers Enzyme Stability Freeze Drying Gels Hydrogen-Ion Concentration Microscopy, Electron, Scanning Pectins Solubility Trehalose |
spellingShingle |
Air drying Alginate beads Bead structures Composition changes Controlled release Drying methods Enzyme release Fickian diffusion Polymer chains Release kinetics Release mechanism Coagulation Enzyme activity Freezing Gelation Heat treatment Vacuum Drying alginate bead alginic acid beta cyclodextrin beta fructofuranosidase pectin trehalose unclassified drug article chemical composition chemical structure desiccation diffusion encapsulation enzyme activity enzyme kinetics enzyme release enzyme stability freeze drying freezing gelation heat treatment pH porosity priority journal vacuum Alginates beta-Cyclodextrins beta-Fructofuranosidase Calorimetry, Differential Scanning Delayed-Action Preparations Desiccation Drug Carriers Enzyme Stability Freeze Drying Gels Hydrogen-Ion Concentration Microscopy, Electron, Scanning Pectins Solubility Trehalose Santagapita, Patricio Román Mazzobre, María Florencia Buera, María del Pilar Formulation and drying of alginate beads for controlled release and stabilization of invertase |
topic_facet |
Air drying Alginate beads Bead structures Composition changes Controlled release Drying methods Enzyme release Fickian diffusion Polymer chains Release kinetics Release mechanism Coagulation Enzyme activity Freezing Gelation Heat treatment Vacuum Drying alginate bead alginic acid beta cyclodextrin beta fructofuranosidase pectin trehalose unclassified drug article chemical composition chemical structure desiccation diffusion encapsulation enzyme activity enzyme kinetics enzyme release enzyme stability freeze drying freezing gelation heat treatment pH porosity priority journal vacuum Alginates beta-Cyclodextrins beta-Fructofuranosidase Calorimetry, Differential Scanning Delayed-Action Preparations Desiccation Drug Carriers Enzyme Stability Freeze Drying Gels Hydrogen-Ion Concentration Microscopy, Electron, Scanning Pectins Solubility Trehalose |
description |
Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or air drying), and thermal treatment were evaluated on invertase stability and its release kinetics from beads. The enzyme release mechanism from wet beads depended on pH. The addition of trehalose, pectin, and β-cyclodextrin modified the bead structure, leading in some cases to a release mechanism that included the relaxation of the polymer chains, besides Fickian diffusion. Enzyme release from vacuum-dried beads was much faster than from freeze-dried beads, probably due to their higher pore size. The inclusion of β-cyclodextrin and especially of pectin prevented enzyme activity losses during bead generation, and trehalose addition was fundamental for achieving adequate invertase protection during freezing, drying, and thermal treatment. Present results showed that several alternatives such as drying method, composition, as well as pH of the relese medium can be managed to control enzyme release. © 2011 American Chemical Society. |
author |
Santagapita, Patricio Román Mazzobre, María Florencia Buera, María del Pilar |
author_facet |
Santagapita, Patricio Román Mazzobre, María Florencia Buera, María del Pilar |
author_sort |
Santagapita, Patricio Román |
title |
Formulation and drying of alginate beads for controlled release and stabilization of invertase |
title_short |
Formulation and drying of alginate beads for controlled release and stabilization of invertase |
title_full |
Formulation and drying of alginate beads for controlled release and stabilization of invertase |
title_fullStr |
Formulation and drying of alginate beads for controlled release and stabilization of invertase |
title_full_unstemmed |
Formulation and drying of alginate beads for controlled release and stabilization of invertase |
title_sort |
formulation and drying of alginate beads for controlled release and stabilization of invertase |
publishDate |
2011 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15257797_v12_n9_p3147_Santagapita http://hdl.handle.net/20.500.12110/paper_15257797_v12_n9_p3147_Santagapita |
work_keys_str_mv |
AT santagapitapatricioroman formulationanddryingofalginatebeadsforcontrolledreleaseandstabilizationofinvertase AT mazzobremariaflorencia formulationanddryingofalginatebeadsforcontrolledreleaseandstabilizationofinvertase AT bueramariadelpilar formulationanddryingofalginatebeadsforcontrolledreleaseandstabilizationofinvertase |
_version_ |
1768542473087352832 |