Lead toxicity in cyanobacterial porphyrin metabolism

The effect of Pb2+ on growth, tetrapyrrole photosynthetic pigment content, total free porphyrin, and 5-aminolevulinate dehydratase (ALA-D) activity of a cyanobacterium, Microchaete tenera, and its ability to sequester Pb2+ from the culture medium were studied. Pb2+ was assayed by graphite furnace at...

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Autores principales:	Zaccaro, María Cristina, Stella de Rosellini, Ana María Cristina
Publicado:	2001
Materias:	5-aminolevulinate dehydratase Cyanobacteria Lead biosorption Lead toxicity Tetrapyrrole pigments bacterial protein chlorophyll graphite lead phycobiliprotein porphobilinogen synthase porphyrin cyanobacterium toxicity article assay atomic absorption spectrometry bacterial growth biomass controlled study culture medium Cyanobacterium enzyme activity enzyme inhibition lead poisoning Microchaete tenera molecular weight nonhuman photosynthesis porphyrin metabolism priority journal stress Lead Photosynthesis Porphobilinogen Synthase Porphyrins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15204081_v16_n1_p61_Zaccaro http://hdl.handle.net/20.500.12110/paper_15204081_v16_n1_p61_Zaccaro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15204081_v16_n1_p61_Zaccaro
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spelling	paper:paper_15204081_v16_n1_p61_Zaccaro2023-06-08T16:18:50Z Lead toxicity in cyanobacterial porphyrin metabolism Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina 5-aminolevulinate dehydratase Cyanobacteria Lead biosorption Lead toxicity Tetrapyrrole pigments bacterial protein chlorophyll graphite lead phycobiliprotein porphobilinogen synthase porphyrin cyanobacterium lead toxicity article assay atomic absorption spectrometry bacterial growth biomass controlled study culture medium Cyanobacterium enzyme activity enzyme inhibition lead poisoning Microchaete tenera molecular weight nonhuman photosynthesis porphyrin metabolism priority journal stress Cyanobacteria Lead Photosynthesis Porphobilinogen Synthase Porphyrins Cyanobacteria Microchaete tenera The effect of Pb2+ on growth, tetrapyrrole photosynthetic pigment content, total free porphyrin, and 5-aminolevulinate dehydratase (ALA-D) activity of a cyanobacterium, Microchaete tenera, and its ability to sequester Pb2+ from the culture medium were studied. Pb2+ was assayed by graphite furnace atomic absorption spectrophotometry. M. tenera growth and chlorophyll a content were not affected by 0.5, 1.0, and 6.0 ppm of Pb2+. These treatments doubled the protein content and increased the phycobiliprotein content by four times after 7 days. The ALA-D activity decreased in all concentrations by 63% at day 7 and by 34% at day 14. As a consequence of ALA-D inhibition, total free porphyrin also decreased by 64% at day 7 and by 40% at day 14. The highest biomass lead uptake (7454±565 μg Pb2+/g dry weight) was observed at day 3 with 6.0 ppm of Pb2+ in the culture medium. Uptake coefficient was highest (3723±279 μg Pb2+g-1 dry weight/ppm of applied Pb2+) with 1.0 ppm after 3 days. The increase in protein and antenna pigments on day 7 was probably a response to stress conditions and could explain why the toxic metal did not affect growth. ALA-D inhibition and high lead biomass content confirm the importance of this enzyme as a biological indicator for stress. © 2001 by John Wiley & Sons, Inc. Fil:Zaccaro, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15204081_v16_n1_p61_Zaccaro http://hdl.handle.net/20.500.12110/paper_15204081_v16_n1_p61_Zaccaro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-aminolevulinate dehydratase Cyanobacteria Lead biosorption Lead toxicity Tetrapyrrole pigments bacterial protein chlorophyll graphite lead phycobiliprotein porphobilinogen synthase porphyrin cyanobacterium lead toxicity article assay atomic absorption spectrometry bacterial growth biomass controlled study culture medium Cyanobacterium enzyme activity enzyme inhibition lead poisoning Microchaete tenera molecular weight nonhuman photosynthesis porphyrin metabolism priority journal stress Cyanobacteria Lead Photosynthesis Porphobilinogen Synthase Porphyrins Cyanobacteria Microchaete tenera
spellingShingle	5-aminolevulinate dehydratase Cyanobacteria Lead biosorption Lead toxicity Tetrapyrrole pigments bacterial protein chlorophyll graphite lead phycobiliprotein porphobilinogen synthase porphyrin cyanobacterium lead toxicity article assay atomic absorption spectrometry bacterial growth biomass controlled study culture medium Cyanobacterium enzyme activity enzyme inhibition lead poisoning Microchaete tenera molecular weight nonhuman photosynthesis porphyrin metabolism priority journal stress Cyanobacteria Lead Photosynthesis Porphobilinogen Synthase Porphyrins Cyanobacteria Microchaete tenera Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina Lead toxicity in cyanobacterial porphyrin metabolism
topic_facet	5-aminolevulinate dehydratase Cyanobacteria Lead biosorption Lead toxicity Tetrapyrrole pigments bacterial protein chlorophyll graphite lead phycobiliprotein porphobilinogen synthase porphyrin cyanobacterium lead toxicity article assay atomic absorption spectrometry bacterial growth biomass controlled study culture medium Cyanobacterium enzyme activity enzyme inhibition lead poisoning Microchaete tenera molecular weight nonhuman photosynthesis porphyrin metabolism priority journal stress Cyanobacteria Lead Photosynthesis Porphobilinogen Synthase Porphyrins Cyanobacteria Microchaete tenera
description	The effect of Pb2+ on growth, tetrapyrrole photosynthetic pigment content, total free porphyrin, and 5-aminolevulinate dehydratase (ALA-D) activity of a cyanobacterium, Microchaete tenera, and its ability to sequester Pb2+ from the culture medium were studied. Pb2+ was assayed by graphite furnace atomic absorption spectrophotometry. M. tenera growth and chlorophyll a content were not affected by 0.5, 1.0, and 6.0 ppm of Pb2+. These treatments doubled the protein content and increased the phycobiliprotein content by four times after 7 days. The ALA-D activity decreased in all concentrations by 63% at day 7 and by 34% at day 14. As a consequence of ALA-D inhibition, total free porphyrin also decreased by 64% at day 7 and by 40% at day 14. The highest biomass lead uptake (7454±565 μg Pb2+/g dry weight) was observed at day 3 with 6.0 ppm of Pb2+ in the culture medium. Uptake coefficient was highest (3723±279 μg Pb2+g-1 dry weight/ppm of applied Pb2+) with 1.0 ppm after 3 days. The increase in protein and antenna pigments on day 7 was probably a response to stress conditions and could explain why the toxic metal did not affect growth. ALA-D inhibition and high lead biomass content confirm the importance of this enzyme as a biological indicator for stress. © 2001 by John Wiley & Sons, Inc.
author	Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina
author_facet	Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina
author_sort	Zaccaro, María Cristina
title	Lead toxicity in cyanobacterial porphyrin metabolism
title_short	Lead toxicity in cyanobacterial porphyrin metabolism
title_full	Lead toxicity in cyanobacterial porphyrin metabolism
title_fullStr	Lead toxicity in cyanobacterial porphyrin metabolism
title_full_unstemmed	Lead toxicity in cyanobacterial porphyrin metabolism
title_sort	lead toxicity in cyanobacterial porphyrin metabolism
publishDate	2001
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15204081_v16_n1_p61_Zaccaro http://hdl.handle.net/20.500.12110/paper_15204081_v16_n1_p61_Zaccaro
work_keys_str_mv	AT zaccaromariacristina leadtoxicityincyanobacterialporphyrinmetabolism AT stelladerosellinianamariacristina leadtoxicityincyanobacterialporphyrinmetabolism
_version_	1768544100274929664

Lead toxicity in cyanobacterial porphyrin metabolism

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