Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell respon...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1478811X_v11_n1_p_Giudice http://hdl.handle.net/20.500.12110/paper_1478811X_v11_n1_p_Giudice |
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paper:paper_1478811X_v11_n1_p_Giudice2023-06-08T16:18:23Z Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B Giudice, Jimena Giordano, Luciana Jares, Elizabeth Andrea Coluccio Leskow, Federico Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses. Results: We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. We combined biotinylated ligands with streptavidin conjugated quantum dots and visible fluorescent proteins to visualize the binding of IGF-II and insulin to IR-B and their ensuing internalization. By confocal microscopy and flow cytometry in living cells, we studied the internalization kinetic through the IR-B of both IGF-II, known to elicit proliferative responses, and insulin, a regulator of metabolism. Conclusions: IGF-II promotes a faster internalization of IR-B than insulin. We propose that IGF-II differentially activates mitogenic responses through endosomes, while insulin-activated IR-B remains at the plasma membrane. This fact could facilitate the interaction with key effector molecules involved in metabolism regulation. © 2013 Giudice et al.; licensee BioMed Central Ltd. Fil:Giudice, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giordano, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coluccio Leskow, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1478811X_v11_n1_p_Giudice http://hdl.handle.net/20.500.12110/paper_1478811X_v11_n1_p_Giudice |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction |
| spellingShingle |
Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction Giudice, Jimena Giordano, Luciana Jares, Elizabeth Andrea Coluccio Leskow, Federico Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B |
| topic_facet |
Endocytosis Insulin receptor Insulin/IGF-II Microscopy Quantum dots Signaling cyan fluorescent protein insulin insulin receptor insulin receptor B quantum dot somatomedin B streptavidin unclassified drug article biotinylation cell membrane cell proliferation confocal microscopy controlled study endocytosis endosome flow cytometry gene overexpression genetic transcription human human cell internalization metabolic regulation mitogenesis priority journal protein interaction receptor binding receptor upregulation signal transduction |
| description |
Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses. Results: We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. We combined biotinylated ligands with streptavidin conjugated quantum dots and visible fluorescent proteins to visualize the binding of IGF-II and insulin to IR-B and their ensuing internalization. By confocal microscopy and flow cytometry in living cells, we studied the internalization kinetic through the IR-B of both IGF-II, known to elicit proliferative responses, and insulin, a regulator of metabolism. Conclusions: IGF-II promotes a faster internalization of IR-B than insulin. We propose that IGF-II differentially activates mitogenic responses through endosomes, while insulin-activated IR-B remains at the plasma membrane. This fact could facilitate the interaction with key effector molecules involved in metabolism regulation. © 2013 Giudice et al.; licensee BioMed Central Ltd. |
| author |
Giudice, Jimena Giordano, Luciana Jares, Elizabeth Andrea Coluccio Leskow, Federico |
| author_facet |
Giudice, Jimena Giordano, Luciana Jares, Elizabeth Andrea Coluccio Leskow, Federico |
| author_sort |
Giudice, Jimena |
| title |
Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B |
| title_short |
Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B |
| title_full |
Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B |
| title_fullStr |
Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B |
| title_full_unstemmed |
Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B |
| title_sort |
insulin and insulin like growth factor ii endocytosis and signaling via insulin receptor b |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1478811X_v11_n1_p_Giudice http://hdl.handle.net/20.500.12110/paper_1478811X_v11_n1_p_Giudice |
| work_keys_str_mv |
AT giudicejimena insulinandinsulinlikegrowthfactoriiendocytosisandsignalingviainsulinreceptorb AT giordanoluciana insulinandinsulinlikegrowthfactoriiendocytosisandsignalingviainsulinreceptorb AT jareselizabethandrea insulinandinsulinlikegrowthfactoriiendocytosisandsignalingviainsulinreceptorb AT coluccioleskowfederico insulinandinsulinlikegrowthfactoriiendocytosisandsignalingviainsulinreceptorb |
| _version_ |
1768544518288703488 |